2,578 research outputs found
Patchy Interspecific Sequence Similarities Efficiently Identify Positive cis-Regulatory Elements in the Sea Urchin
We demonstrate that interspecific sequence conservation can provide a systematic guide to the identification of functional cis-regulatory elements within a large expanse of genomic DNA. The test was carried out on the otx gene of Strongylocentrotus purpuratus. This gene plays a major role in the gene regulatory network that underlies endomesoderm specification in the embryo. The cis-regulatory organization of the otx gene is expected to be complex, because the gene has three different start sites (X. Li, C.-K. Chuang, C.-A. Mao, L. M. Angerer, and W. H. Klein, 1997, Dev. Biol. 187, 253–266), and it is expressed in many different spatial domains of the embryo. BAC recombinants containing the otx gene were isolated from Strongylocentrotus purpuratus and Lytechinus variegatus libraries, and the ordered sequence of these BACs was obtained and annotated. Sixty kilobases of DNA flanking the gene, and included in the BAC sequence from both species, were scanned computationally for short conserved sequence elements. For this purpose, we used a newly constructed software package assembled in our laboratory, “FamilyRelations.” This tool allows detection of sequence similarities above a chosen criterion within sliding windows set at 20–50 bp. Seventeen partially conserved regions, most a few hundred base pairs long, were amplified from the S. purpuratus BAC DNA by PCR, inserted in an expression vector driving a CAT reporter, and tested for cis-regulatory activity by injection into fertilized S. purpuratus eggs. The regulatory activity of these constructs was assessed by whole-mount in situ hybridization (WMISH) using a probe against CAT mRNA. Of the 17 constructs, 11 constructs displayed spatially restricted regulatory activity, and 6 were inactive in this test. The domains within which the cis-regulatory constructs were expressed are approximately consistent with results from a WMISH study on otx expression in the embryo, in which we used probes specific for the mRNAs generated from each of the three transcription start sites. Four separate cis-regulatory elements that specifically produce endomesodermal expression were identified, as well as ubiquitously active elements, and ectoderm-specific elements. We confirm predictions from other work with respect to target sites for specific transcription factors within the elements that express in the endoderm
The Rapid Outbursting Star GM Cep: An EX-or in Tr 37?
We present optical, IR and millimeter observations of the solar-type star
13-277, also known as GM Cep, in the 4 Myr-old cluster Tr 37. GM Cep
experiences rapid magnitude variations of more than 2 mag at optical
wavelengths. We explore the causes of the variability, which seem to be
dominated by strong increases in the accretion, being similar to EX-or
episodes. The star shows high, variable accretion rates (up to ~10
Msun/yr), signs of powerful winds, and it is a very fast rotator (Vsini~43
km/s). Its strong mid-IR excesses reveal a very flared disk and/or a remnant
envelope, most likely out of hydrostatic equilibrium. The 1.3 millimeter fluxes
suggest a relatively massive disk (Mdisk~0.1 Msun). Nevertheless, the
millimeter mass is not enough to sustain increased accretion episodes over
large timescales, unless the mass is underestimated due to significant grain
growth. We finally explore the possibility of GM Cep having a binary companion,
which could trigger disk instabilities producing the enhanced accretion
episodes.Comment: 43 pages, including 10 figures, ApJ in pres
Characterising the Gravitational Instability in Cooling Accretion Discs
We perform numerical analyses of the structure induced by gravitational
instabilities in cooling gaseous accretion discs. For low enough cooling rates
a quasi-steady configuration is reached, with the instability saturating at a
finite amplitude in a marginally stable disc. We find that the saturation
amplitude scales with the inverse square root of the cooling parameter beta =
t_cool / t_dyn, which indicates that the heating rate induced by the
instability is proportional to the energy density of the induced density waves.
We find that at saturation the energy dissipated per dynamical time by weak
shocks due is of the order of 20 per cent of the wave energy. From Fourier
analysis of the disc structure we find that while the azimuthal wavenumber is
roughly constant with radius, the mean radial wavenumber increases with radius,
with the dominant mode corresponding to the locally most unstable wavelength.
We demonstrate that the density waves excited in relatively low mass discs are
always close to co-rotation, deviating from it by approximately 10 per cent.
This can be understood in terms of the flow Doppler-shifted phase Mach number
-- the pattern speed self-adjusts so that the flow into spiral arms is always
sonic. This has profound effects on the degree to which transport through
self-gravity can be modelled as a viscous process. Our results thus provide (a)
a detailed description of how the self-regulation mechanism is established for
low cooling rates, (b) a clarification of the conditions required for
describing the transport induced by self-gravity through an effective
viscosity, (c) an estimate of the maximum amplitude of the density perturbation
before fragmentation occurs, and (d) a simple recipe to estimate the density
perturbation in different thermal regimes.Comment: 16 pages, 22 figures. Accepted for publication in MNRAS 11 November
200
Crystallographic structure of ultrathin Fe films on Cu(100)
We report bcc-like crystal structures in 2-4 ML Fe films grown on fcc Cu(100)
using scanning tunneling microscopy. The local bcc structure provides a
straightforward explanation for their frequently reported outstanding magnetic
properties, i.e., ferromagnetic ordering in all layers with a Curie temperature
above 300 K. The non-pseudomorphic structure, which becomes pseudomorphic above
4 ML film thickness is unexpected in terms of conventional rules of thin film
growth and stresses the importance of finite thickness effects in ferromagnetic
ultrathin films.Comment: 4 pages, 3 figures, RevTeX/LaTeX2.0
An Exactly Solvable Model of N Coupled Luttinger Chains
We calculate the exact Green function of a special model of N coupled
Luttinger chains with arbitrary interchain hopping t_{perp}. The model is
exactly solvable via bosonization if the interchain interaction does not fall
off in the direction perpendicular to the chains. For any finite N we find
Luttinger liquid behavior and explicitly calculate the anomalous dimension
gamma^(N). However, the Luttinger liquid state does not preclude coherent
interchain hopping. We also show that gamma^(N) -> 0 for N -> infinity, so that
in the limit of infinitely many chains we obtain a Fermi liquid.Comment: accepted for publication in Phys. Rev.
Performance of Newtonian filters in detecting gravitational waves from coalescing binaries
Coalescing binary systems are one of the most promising sources of
gravitational waves. The technique of matched filtering used in the detection
of gravitational waves from coalescing binaries relies on the construction of
accurate templates. Until recently filters modelled on the quadrupole or the
Newtonian approximation were deemed sufficient. Recently it was shown that
post-Newtonian effects contribute to a secular growth in the phase difference
between the actual signal and its corresponding Newtonian template. In this
paper we investigate the possibility of compensating for the phase difference
caused by the post-Newtonian terms by allowing for a shift in the Newtonian
filter parameters. We find that Newtonian filters perform adequately for the
purpose of detecting the presence of the signal for both the initial and the
advanced LIGO detectors.Comment: Revtex 9 pages + 6 figures ( Can be obtained by "anonymous" ftp from
144.16.31.1 in dir /pub/rbs. Submitted to Physical Review D. IUCAA 1
The initiator methionine tRNA drives secretion of type II collagen from stromal fibroblasts to promote tumor growth and angiogenesis
Summary:
Expression of the initiator methionine tRNA (tRNAi
Met)
is deregulated in cancer. Despite this fact, it is not
currently known how tRNAi
Met expression levels influence
tumor progression. We have found that tRNAi
Met
expression is increased in carcinoma-associated
fibroblasts, implicating deregulated expression of
tRNAi
Met in the tumor stroma as a possible contributor
to tumor progression. To investigate how elevated
stromal tRNAi
Met contributes to tumor progression,
we generated a mouse expressing additional copies
of the tRNAi
Met gene (2+tRNAi
Met mouse). Growth
and vascularization of subcutaneous tumor allografts
was enhanced in 2+tRNAi
Met mice compared with
wild-type littermate controls. Extracellular matrix
(ECM) deposited by fibroblasts from 2+tRNAi
Met
mice supported enhanced endothelial cell and fibroblast
migration. SILAC mass spectrometry indicated
that elevated expression of tRNAi
Met significantly
increased synthesis and secretion of certain types of
collagen, in particular type II collagen. Suppression
of type II collagen opposed the ability of tRNAi
Metoverexpressing
fibroblasts to deposit pro-migratory
ECM. We used the prolyl hydroxylase inhibitor ethyl-
3,4-dihydroxybenzoate (DHB) to determine whether
collagen synthesis contributes to the tRNAi
Met-driven
pro-tumorigenic stroma in vivo. DHB had no effect
on the growth of syngeneic allografts in wild-type
mice but opposed the ability of 2+tRNAi
Met mice to
support increased angiogenesis and tumor growth.
Finally, collagen II expression predicts poor prognosis
in high-grade serous ovarian carcinoma. Taken
together, these data indicate that increased tRNAi
Met
levels contribute to tumor progression by enhancing
the ability of stromal fibroblasts to synthesize and
secrete a type II collagen-rich ECM that supports
endothelial cell migration and angiogenesis
A Method for Calculating the Structure of (Singular) Spacetimes in the Large
A formalism and its numerical implementation is presented which allows to
calculate quantities determining the spacetime structure in the large directly.
This is achieved by conformal techniques by which future null infinity
(\Scri{}^+) and future timelike infinity () are mapped to grid points on
the numerical grid. The determination of the causal structure of singularities,
the localization of event horizons, the extraction of radiation, and the
avoidance of unphysical reflections at the outer boundary of the grid, are
demonstrated with calculations of spherically symmetric models with a scalar
field as matter and radiation model.Comment: 29 pages, AGG2
Genetic loci on chromosome 5 are associated with circulating levels of interleukin-5 and eosinophil count in a European population with high risk for cardiovascular disease
IL-5 is a Th2 cytokine which activates eosinophils and is suggested to have an atheroprotective role. Genetic variants in the IL5 locus have been associated with increased risk of CAD and ischemic stroke. In this study we aimed to identify genetic variants associated with IL-5 concentrations and apply a Mendelian randomisation approach to assess IL-5 levels for causal effect on intima-media thickness in a European population at high risk of coronary artery disease. We analysed SNPs within robustly associated candidate loci for immune, inflammatory, metabolic and cardiovascular traits. We identified 2 genetic loci for IL-5 levels (chromosome 5, rs56183820, BETA = 0.11, P = 6.73E−5 and chromosome 14, rs4902762, BETA = 0.12, P = 5.76E−6) and one for eosinophil count (rs72797327, BETA = −0.10, P = 1.41E−6). Both chromosome 5 loci were in the vicinity of the IL5 gene, however the association with IL-5 levels failed to replicate in a meta-analysis of 2 independent cohorts (rs56183820, BETA = 0.04, P = 0.2763, I2 = 24, I2 − P = 0.2516). No significant associations were observed between SNPs associated with IL-5 levels or eosinophil count and IMT measures. Expression quantitative trait analyses indicate effects of the IL-5 and eosinophil-associated SNPs on RAD50 mRNA expression levels (rs12652920 (r2 = 0.93 with rs56183820) BETA = −0.10, P = 8.64E−6 and rs11739623 (r2 = 0.96 with rs72797327) BETA = −0.23, P = 1.74E−29, respectively). Our data do not support a role for IL-5 levels and eosinophil count in intima-media thickness, however SNPs associated with IL-5 and eosinophils might influence stability of the atherosclerotic plaque via modulation of RAD50 levels
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