Keratocystic odontogenic tumour: An experience in the northeast of Brazil

Introduction. Keratocystic odontogenic tumours raise particular interest, because of their high recurrence rate and association with nevoid basal cell carcinoma syndrome. Objective. To analyze the clinical and histopathological features of all cases diagnosed as keratocystic odontogenic tumour in a Brazilian population. Methods. A total of 64 keratocystic odontogenic tumours, arising in forty-six patients, were evaluated using the following parameters: association with nevoid basal cell carcinoma syndrome, gender, age at first diagnosis, race, anatomical location, symptoms, radiographic features, history of recurrence, association with teeth, and treatment. Results. Keratocystic odontogenic tumours were more frequent among women than men (1:0.84). The mean patient age was 31.5 years (SD: ±16.6). Ten tumours (16.4%) involved the maxilla and 51 (83.6%) the mandible. Swelling (n=12; 46.1%), followed by pain and swelling (n=4; 15.3%), were most common clinical manifestations. A unilocular radiotransparency with well-defined margins was the main radiographic finding (n=29; 87.8%). A significant association was observed between the multilocular radiographic pattern and recurrence (p<0.05, Fisher’s Test). Sixty-one (95.3%) tumours were treated by surgical enucleation followed by bone curettage, and the recurrence rate was 13% (n=6). This study showed that the keratocystic odontogenic tumours relapsed within a mean period of 25-36 months. Conclusion. Despite the results of this study being similar to previous reports found in the literature, it provides an important insight about keratocystic odontogenic tumours in a Brazilian population

Imunodetecção das proteínas Ki-67, p53, p63 e laminina em ceratocisto odontogênico: associação a aspectos clínicos e imunológicos

Submitted by Repositório Arca ([email protected]) on 2019-07-08T18:54:13Z No. of bitstreams: 1 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2019-07-12T16:26:43Z (GMT) No. of bitstreams: 2 Clarissa Araujo Silva Gurgel Imunodetecção...2006.pdf: 51231743 bytes, checksum: eeaa38246f9cfea180c457302f5cab19 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Made available in DSpace on 2019-07-12T16:26:43Z (GMT). No. of bitstreams: 2 Clarissa Araujo Silva Gurgel Imunodetecção...2006.pdf: 51231743 bytes, checksum: eeaa38246f9cfea180c457302f5cab19 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2006CNPqUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.O Ceratocisto Odontogênico (CO) é uma patologia distinta do complexo estomatognático, especialmente pelo comportamento clínico agressivo, com uma alta taxa de recidiva, comprometimento de estruturas anatômicas nobres, crescimento infiltrativo e características biológicas distintas dos demais cistos odontogênicos. O presente trabalho teve como objetivo estudar a expressão imuno-histoquímica dos marcadores de proliferação celular, Ki-67, p53 e p63 e de matriz extracelular, laminina, em COs, bem como associá-los a aspectos clínicos e imaginológicos, na tentativa de melhor compreender os mecanismos moleculares envolvidos no comportamento biológico desta patologia. Trinta e sete COs de trinta pacientes foram submetidos a técnica imunohistoquímica para os anticorpos supracitados, utilizando o sistema EnVision™. Os resultados clínicos, imaginológicos, histomorfológicos e imuno-histoquímicos foram compilados e associados estatisticamente. A faixa etária de dez a vinte anos (33,3%) e melanodermas (60%) compuseram a maior parte desta amostra e a região posterior da mandíbula foi a mais acometida (83,8%). Observou-se uma associação estatisticamente significante entre a presença de cistos satélites e padrão imaginológico multilocular (p= 0,0167), distorção do canal mandibular (p= 0,0243) e recidivas (p= 0,0033). Para os anticorpos Ki-67 e p53, as células imunomarcadas localizavam-se predominantemente nas camadas supra-basais, enquanto a proteína p63 distribuía-se por todo o epitélio de revestimento cístico. Ao mesmo tempo, não se observou diferença na marcação para estas proteínas, entre lesões primárias e recorrentes (Ki- 67, p= 0,5591; p53, p= 0,9847; p63, p= 0,9127) e aquelas associadas a SCBCN (Ki-67, p= 0,7013; p53, p= 0,3197; p63, p= 0,2427). A expressão da laminina foi predominantemente contínua (n=18) e intensa (n=12), inclusive em áreas de desprendimento epitelial e acantose. Em áreas de inflamação, observou-se o esmaecimento desta imunomarcação e uma maior expressão do Ki-67 (p< 0,05), sem afetar a expressão das proteínas p53 e p63. Estes resultados corroboram com a hipótese da existência de um compartimento proliferativo supra-basal no epitélio do CO, o qual pode contribuir para comportamentos biológico e clínico mais agressivos para esta patologia. Ao mesmo tempo, sugerem a necessidade de mais investigações que abordem as interações entre os componentes da matriz extracelular e ceratinócitos, além de estudos prospectivos que possibilitem associar os achados clínicos e imuno- histoquímicos, na tentativa de estabelecer marcadores de prognóstico para esta patologia.Odontogenic Keratocyst (OK) represents a distinct lesion of the jaws, especially due to the aggressive clinical behaviour, and high recurrences rates. These features make it distinghishable from other odontogenic cysts. The aim of this study was to analyse the proliferation markers such as Ki-67, p53 and p63 and laminin in attempt to evaluate the biological behavior of OK as well as associating them with clinical and images features. Using immunohistochemical technique with EnVision™, thirty and seven cases of COs were studied. Clinical, images, histomorphological and immunohistochemical data were compiled and submitted to statistical analysis. Our results showed that black young adults preponderated in the most of the sample and the body-angle- ramus area of mandible was the most common site of occurrence. A statistically significant association was observed between microkeratocysts, multilocular lesions (p= 0, 0167), distortion of mandibular canal (p= 0,0243) and recurrences (p= 0,0033). Positive cells ratio for ki-67 and p53 was more evident in the parabasal epithelial cells and p63 protein distribution involved all epithelial layers. The lesions immunolabeled for Ki-67, p53 and p63 proteins did not show a statistically significant difference between primary and recurrent OK (Ki-67, p= 0,5591; p53, p= 0,9847; p63, p= 0,9127). Similarly, this was detected when these lesions were associated to basal cell naevus syndrome (Ki- 67, p= 0,7013; p53, p= 0,3197; p63, p= 0,2427). Laminin was predominantly continuous (n=18) and intense (n=12), including those areas that exhibited disruption of the epithelium and acanthosis. A statistically significant increase of Ki-67 positive cells (p< 0,05) was observed in inflamed OK, without affecting the p53 and p63 expression. Our results suggest that Ki-67, p53 and p63 expression reflects a proliferative cellular activity in epithelium lining of OK, contributing for a more aggressive biological behaviour of it. In addition, other prospectives studies are useful to associate the clinical and imunohistochemical features in attempt to establish prognostic markers for this entity

Density of Langerhans cells in the keratocystic odontogenic tumor Density of Langerhans cells in the keratocystic odontogenic tumor Densidade das células de Langerhans no tumor odontogênico queratocístico

Introduction: Keratocystic odontogenic tumors (KOTs) are distinct odontogenic lesions commonly affecting the mandible bones. Langerhans cells (LCs) are specialized dendritic cells responsible for the presentation of antigens to T lymphocytes in mucosal and cutaneous surfaces. Objective: This study analyzed the immunohistochemical expression of LCs in KOTs. Material and method: Fifteen cases of KOTs were studied using the anti-CD1a marker. Results: LCs were observed in all 15 cases analyzed. They were found to be concentrated in areas of cystic epithelial hyperplasia, mainly in those areas presenting higher concentration of inflammatory cells. Furthermore, a significant association between the number of LCs and areas of cystic epithelium presenting hyperplasia (Mann-Whitney test, p = 0.0223) was observed. The shape and location of these cells in KOTs epithelium were variable. Conclusion: The lower number of LCs observed on atrophic cystic epithelium of KOTs may be due to decreased epithelial immunosurveillance and this may result in locally aggressive invasiveness

Sclerosing Polycystic Adenosis of the minor salivary gland: case report

Ramos, Eduardo Antônio Gonçalves. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-04-27T19:00:24Z No. of bitstreams: 1 Gurgel CA Adenose Policística Esclerosante....pdf: 221346 bytes, checksum: 8845b8c234e4217666a3e8c85f36ae0c (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-04-27T19:08:20Z (GMT) No. of bitstreams: 1 Gurgel CA Adenose Policística Esclerosante....pdf: 221346 bytes, checksum: 8845b8c234e4217666a3e8c85f36ae0c (MD5)Made available in DSpace on 2018-04-27T19:08:20Z (GMT). No. of bitstreams: 1 Gurgel CA Adenose Policística Esclerosante....pdf: 221346 bytes, checksum: 8845b8c234e4217666a3e8c85f36ae0c (MD5) Previous issue date: 2010FAPESBUniversidade Federal da Bahia. Faculdade de Odontologia Laboratório de Patologia Cirúrgica. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Odontologia Laboratório de Patologia Cirúrgica. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Odontologia Laboratório de Patologia Cirúrgica. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Odontologia Laboratório de Patologia Cirúrgica. Salvador, BA, Brasi

Association Between Mast Cells and Collagen Maturation in Chronic Periodontitis in Humans

Rocha, Clarissa Araújo Gurgel. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-17T12:12:40Z No. of bitstreams: 1 Ribeiro LS Association Between Mast Cells and Collagen....pdf: 869772 bytes, checksum: 1f5a293371ad8f86f3df2b8382533b4e (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-17T12:22:47Z (GMT) No. of bitstreams: 1 Ribeiro LS Association Between Mast Cells and Collagen....pdf: 869772 bytes, checksum: 1f5a293371ad8f86f3df2b8382533b4e (MD5)Made available in DSpace on 2018-08-17T12:22:47Z (GMT). No. of bitstreams: 1 Ribeiro LS Association Between Mast Cells and Collagen....pdf: 869772 bytes, checksum: 1f5a293371ad8f86f3df2b8382533b4e (MD5) Previous issue date: 2018National Council for Scientific and Technological Development (303003/2015-4).Federal University of Bahia. Department of Oral Pathology. Postgraduate Program in Health and Dentistry. Salvador, BA, Brazil / Federal University of Bahia. School of Dentistry. Department of Periodontics. Salvador, BA, BrazilFederal University of Bahia. Department of Oral Pathology. Postgraduate Program in Health and Dentistry. Salvador, BA, Brazil / Federal University of Bahia. School of Dentistry. Department of Periodontics. Salvador, BA, BrazilFederal University of Bahia. Department of Oral Pathology. Postgraduate Program in Health and Dentistry. Salvador, BA, Brazil / Federal University of Bahia. School of Dentistry. Department of Periodontics. Salvador, BA, BrazilFederal University of Bahia. Department of Oral Pathology. Postgraduate Program in Health and Dentistry. Salvador, BA, Brazil / Federal University of Bahia. School of Dentistry. Department of Periodontics. Salvador, BA, BrazilMast cells (MCs) can influence the maturation of collagen fibers. This study evaluated the relationship between the distribution and degranulation of MCs and collagen maturation in human gingival tissue in chronic periodontitis. A total of 16 specimens of patients clinically diagnosed as periodontitis and 18 controls clinically diagnosed as healthy or gingivitis were included. Immunohistochemistry and Picrosirius staining were performed to identify MCs and assess collagen fibers, respectively. Chi-square, t test, and Pearson's correlation test ( p<0.05) were used. In control specimens, there was a positive association between MCs in the connective tissue and the presence of immature collagen ( p=0.001); in periodontitis samples, this association was not confirmed ( p≥0.12). There was no significant relationship between periodontal diagnosis and collagen maturation or MC degranulation ( p≥0.35). MC density was significantly higher ( p=0.04) in periodontitis tissue (339.01 ± 188.94 MCs/mm2) than in control tissue (211.14 ± 131.13 MCs/mm2) in the area of connective tissue containing inflammatory infiltrate. There was a correlation between the number of MCs and probing depth ( r = 0.34, p=0.04). MCs are involved in the pathogenesis of periodontal diseases and might be associated with collagen maturation in periodontal tissue during the early stages of periodontal disease pathogenesis

Prevalência de lesões bucais associadas ao uso de próteses dentárias removíveis em um serviço de estomatologia

As próteses dentárias removíveis restabelecem a função e estética do sistema estomatognático, embora possam causar injúrias aos tecidos bucais. O objetivo deste estudo é avaliar a prevalência de lesões bucais relacionadas ao uso de próteses dentárias removíveis. Foi realizada a revisão de prontuários dos pacientes atendidos no Serviço de Estomatologia da Faculdade de Odontologia da Universidade Federal da Bahia, no periodo de junho de 2006 à dezembro de 2007. Dos 28 indivíduos usuários de próteses dentárias removíveis, 25 (89,3 %) apresentaram alguma alteração na mucosa bucal, sendo que 42,8 % destas apresentavam-se mal-adaptadas. A hiperplasia fibrobrosa inflamatória (29,42 %), candidíase crônica atrófica (20,6 %), queilite angular (8,82 %) e úlcera traumática (8,82 %) foram as lesões mais prevalentes, e a mucosa alveolar (35,3 %) e o palato duro (29,4 %) os sítios anatômicos mais acometidos. Estes resultados reafirmam a importância da confecção de próteses adequadas, da necessidade de se realizar controles periódicos e fornecer instruções sobre higiene bucal aos pacientes que vierem fazer uso de próteses dentárias removíveis

Activation of sonic hedgehog signaling in oral squamous cell carcinomas: a preliminary study.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-11-19T18:35:20Z No. of bitstreams: 1 Buim CBME Activation of....pdf: 871864 bytes, checksum: 5a38413dbd94658d669d7c54f64b799a (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2014-11-19T18:35:31Z (GMT) No. of bitstreams: 1 Buim CBME Activation of....pdf: 871864 bytes, checksum: 5a38413dbd94658d669d7c54f64b799a (MD5)Made available in DSpace on 2014-11-19T18:50:45Z (GMT). No. of bitstreams: 1 Buim CBME Activation of....pdf: 871864 bytes, checksum: 5a38413dbd94658d669d7c54f64b799a (MD5) Previous issue date: 2011Hospital A.C. Camargo. Department of Anatomical Pathology. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilUniversity of São Paulo. Dental School. Department of General Pathology. São Paulo, SP, BrasilHospital A.C. Camargo. Department of Anatomical Pathology. São Paulo, SP, Brasil / University of São Paulo. Dental School. Department of General Pathology. São Paulo, SP, BrasilSonic hedgehog signaling is important for human development, and aberrant regulation of this pathway can result in the development of tumors. The aim of this study was to examine the expression of sonic hedgehog signaling molecules in oral squamous cell carcinoma. By quantitative real-time polymerase chain reaction, the expression of SHH, SMO, PTCH-1, and GLI-1 was analyzed in 30 oral squamous cell carcinoma cases and 8 samples of nonneoplastic oral mucosa and associated to clinical pathologic features. The expression of β-catenin, cyclin D1, Wnt-1, and Egfr was evaluated by immunohistochemistry in 26 available cases of oral squamous cell carcinoma. Normal oral mucosa from healthy individuals was negative for all genes that were evaluated. SHH, PTCH-1, SMO, and GLI-1 were not expressed in nonneoplastic oral mucosa, and low levels of GLI-1 were observed in nonneoplastic oral mucosa that was adjacent to the tumor. All oral squamous cell carcinoma cases expressed high levels of PTCH-1, SMO, and GLI-1 and were devoid of SHH. The expression of SMO was associated with clinical stage (P = .022) and a borderline association in cervical lymph node metastasis (P = .053). PTCH-1 expression showed a strong correlation with SMO (rs = 0.64; P b .001) and GL-1 (rs = 0.70; P b .001); SMO and GLI-1 also correlated with each other (rs, 0.55; P b .001). All proteins evaluated were expressed as cyclin D1 (92% of samples), β-catenin (73%), Egfr (46%), or Wnt- 1 (32%). Our data demonstrate that sonic hedgehog signaling is activated in oral squamous cell carcinoma and suggest that this pathway mediates its tumorigenesis