Summary of the data for observed peptides analyzed by SRM.

<p>Summary of the data for observed peptides analyzed by SRM.</p

Half-life irradiation doses (D₅₀) and saturating intensity (I_sat) measured for each observed peptide from native and reduced-alkylated form of lysozyme.

<p>CV stands for coefficient of variation.</p

Sequence of the chicken lysozyme with, highlighted in blue, the detected peptides.

<p>Sequence of the chicken lysozyme with, highlighted in blue, the detected peptides.</p

Workflow of the analytical method.

<p>Workflow of the analytical method.</p

Lysozyme tertiary structure (PDB:1AKI).

<p>(a) CELAAAMK is shown in yellow, GTDVQAWIR in green, HGLDNYR in white, GYSLGNWVCAAK in blue, FESNFNTQATNR in pink and WWCNDGR in red. (b) 180° degree rotation around y-axis.</p

Characteristic doses t₁ and t₂ for observed peptides from native and reduced-alkylated form of lysozyme.

<p>CV stands for coefficient of variation.</p

Summary of backbone and side-chain root mean square deviation (RMSD) data for each observed peptide from the native and reduced-alkylated lysozyme.

<p>The results of the two molecular simulation runs are presented separately.</p

Evolution of the signal of the CELAAAMK peptide (a, b) and oxidized CELAAA<i>M</i>K peptide (c, d).

<p>CELAAAMK peptide (3 transitions) irradiated in the native (a) and reduced-alkylated form (b) Oxidized CELAAA<i><u>M</u></i>K peptide (3 transitions) irradiated in the native (c) and reduced-alkylated form (d).</p

Analysis of the reduced-alkylated lysozyme irradiated with a dose at 1000 Gy.

<p>(a) TIC chromatogram, (b) CID spectrum of the CELAAAMK ion <i>m/z</i> 447.2 detected between 8.8 and 9.9 min and (c) CID spectrum of the CELAAA<i><u>M</u></i>K ion <i>m/z</i> 455.2 detected between 6.8 and 7.2 min.</p