2,200 research outputs found
Mechanics of bundled semiflexible polymer networks
While actin bundles are used by living cells for structural fortification,
the microscopic origin of the elasticity of bundled networks is not understood.
Here, we show that above a critical concentration of the actin binding protein
fascin, a solution of actin filaments organizes into a pure network of bundles.
While the elasticity of weakly crosslinked networks is dominated by the affine
deformation of tubes, the network of bundles can be fully understood in terms
of non-affine bending undulations.Comment: 5 pages, 3 figures, final version as publishe
Nonaffine rubber elasticity for stiff polymer networks
We present a theory for the elasticity of cross-linked stiff polymer
networks. Stiff polymers, unlike their flexible counterparts, are highly
anisotropic elastic objects. Similar to mechanical beams stiff polymers easily
deform in bending, while they are much stiffer with respect to tensile forces
(``stretching''). Unlike in previous approaches, where network elasticity is
derived from the stretching mode, our theory properly accounts for the soft
bending response. A self-consistent effective medium approach is used to
calculate the macroscopic elastic moduli starting from a microscopic
characterization of the deformation field in terms of ``floppy modes'' --
low-energy bending excitations that retain a high degree of non-affinity. The
length-scale characterizing the emergent non-affinity is given by the ``fiber
length'' , defined as the scale over which the polymers remain straight.
The calculated scaling properties for the shear modulus are in excellent
agreement with the results of recent simulations obtained in two-dimensional
model networks. Furthermore, our theory can be applied to rationalize bulk
rheological data in reconstituted actin networks.Comment: 12 pages, 10 figures, revised Section II
Entropy of water and the temperature-induced stiffening of amyloid networks
In water, networks of semi-flexible fibrils of the protein -synuclein
stiffen significantly with increasing temperature. We make plausible that this
reversible stiffening is a result of hydrophobic contacts between the fibrils
that become more prominent with increasing temperature. The good agreement of
our experimentally observed temperature dependence of the storage modulus of
the network with a scaling theory linking network elasticity with reversible
crosslinking enables us to quantify the endothermic binding enthalpy and an
estimate the effective size of hydrophobic patches on the fibril surface.Comment: 13 pages, 6 figure
Cytoskeletal polymer networks: Viscoelastic properties are determined by the microscopic interaction potential of cross-links. Biophys
ABSTRACT Although the structure of cross-linking molecules mainly determines the structural organization of actin filaments and with that the static elastic properties of the cytoskeleton, it is largely unknown how the biochemical characteristics of transiently cross-linking proteins (actin-binding proteins (ABPs)) affect the viscoelasticity of actin networks. In this study, we show that the macroscopic network response of reconstituted actin networks can be traced back to the microscopic interaction potential of an individual actin/ABP bond. The viscoelastic response of cross-linked actin networks is set by the cross-linker off-rate, the binding energy, and the characteristic bond length of individual actin/ABP interactions
Project 8 Phase III Design Concept
We present a working concept for Phase III of the Project 8 experiment,
aiming to achieve a neutrino mass sensitivity of ( C.L.)
using a large volume of molecular tritium and a phased antenna array. The
detection system is discussed in detail.Comment: 3 pages, 3 figures, Proceedings of Neutrino 2016, XXVII International
Conference on Neutrino Physics and Astrophysics, 4-9 July 2016, London, U
Results from the Project 8 phase-1 cyclotron radiation emission spectroscopy detector
The Project 8 collaboration seeks to measure the absolute neutrino mass scale
by means of precision spectroscopy of the beta decay of tritium. Our technique,
cyclotron radiation emission spectroscopy, measures the frequency of the
radiation emitted by electrons produced by decays in an ambient magnetic field.
Because the cyclotron frequency is inversely proportional to the electron's
Lorentz factor, this is also a measurement of the electron's energy. In order
to demonstrate the viability of this technique, we have assembled and
successfully operated a prototype system, which uses a rectangular waveguide to
collect the cyclotron radiation from internal conversion electrons emitted from
a gaseous Kr source. Here we present the main design aspects of the
first phase prototype, which was operated during parts of 2014 and 2015. We
will also discuss the procedures used to analyze these data, along with the
features which have been observed and the performance achieved to date.Comment: 3 pages; 2 figures; Proceedings of Neutrino 2016, XXVII International
Conference on Neutrino Physics and Astrophysics, 4-9 July 2016, London, U
Identification and quantification of microplastics in wastewater using focal plane array-based reflectance micro-FT-IR imaging
Microplastics (<5 mm) have been documented in environmental samples on a global scale. While these pollutants may enter aquatic environments via wastewater treatment facilities, the abundance of microplastics in these matrices has not been investigated. Although efficient methods for the analysis of microplastics in sediment samples and marine organisms have been published, no methods have been developed for detecting these pollutants within organic-rich wastewater samples. In addition, there is no standardized method for analyzing microplastics isolated from environmental samples. In many cases, part of the identification protocol relies on visual selection before analysis, which is open to bias. In order to address this, a new method for the analysis of microplastics in wastewater was developed. A pretreatment step using 30% hydrogen peroxide (H2O2) was employed to remove biogenic material, and focal plane array (FPA)-based reflectance micro-Fourier-transform (FT-IR) imaging was shown to successfully image and identify different microplastic types (polyethylene, polypropylene, nylon-6, polyvinyl chloride, polystyrene). Microplastic-spiked wastewater samples were used to validate the methodology, resulting in a robust protocol which was nonselective and reproducible (the overall success identification rate was 98.33%). The use of FPA-based micro-FT-IR spectroscopy also provides a considerable reduction in analysis time compared with previous methods, since samples that could take several days to be mapped using a single-element detector can now be imaged in less than 9 h (circular filter with a diameter of 47 mm). This method for identifying and quantifying microplastics in wastewater is likely to provide an essential tool for further research into the pathways by which microplastics enter the environment.This work is funded by a NERC (Natural Environment Research Council) CASE studentship (NE/K007521/1) with contribution from industrial partner Fera Science Ltd., United Kingdom. The authors would like to thank Peter Vale, from Severn Trent Water Ltd, for providing access to and additionally Ashley Howkins (Brunel University London) for providing travel and assistance with the sampling of the Severn Trent wastewater treatment plant in Derbyshire, UK. We are grateful to Emma Bradley and Chris Sinclair for providing helpful suggestions for our research
Multiple agency perspective, family control, and private information abuse in an emerging economy
Using a comprehensive sample of listed companies in Hong Kong this paper investigates how family control affects private information abuses and firm performance in emerging economies. We combine research on stock market microstructure with more recent studies of multiple agency perspectives and argue that family ownership and control over the board increases the risk of private information abuse. This, in turn, has a negative impact on stock market performance. Family control is associated with an incentive to distort information disclosure to minority shareholders and obtain private benefits of control. However, the multiple agency roles of controlling families may have different governance properties in terms of investors’ perceptions of private information abuse. These findings contribute to our understanding of the conflicting evidence on the governance role of family control within a multiple agency perspectiv
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