2 research outputs found
Analysis of expressed sequence tags from Trypanosoma cruzi amastigotes
A total of 880 expressed sequence tags (EST) originated from clones randomly selected from a Trypanosoma cruzi amastigote cDNA library have been analyzed. Of these, 40% (355 ESTs) have been identified by similarity to sequences in public databases and classified according to functional categorization of their putative products. About 11% of the mRNAs expressed in amastigotes are related to the translational machinery, and a large number of them (9% of the total number of clones in the library) encode ribosomal proteins. A comparative analysis with a previous study, where clones from the same library were selected using sera from patients with Chagas disease, revealed that ribosomal proteins also represent the largest class of antigen coding genes expressed in amastigotes (54% of all immunoselected clones). However, although more than thirty classes of ribosomal proteins were identified by EST analysis, the results of the immunoscreening indicated that only a particular subset of them contains major antigenic determinants recognized by antibodies from Chagas disease patients
Analysis of expressed sequence tags from Trypanosoma cruzi amastigotes
A total of 880 expressed sequence tags (EST) originated from clones
randomly selected from a Trypanosoma cruzi amastigote cDNA
library have been analyzed. Of these, 40% (355 ESTs) have been
identified by similarity to sequences in public databases and
classified according to functional categorization of their putative
products. About 11% of the mRNAs expressed in amastigotes are related
to the translational machinery, and a large number of them (9% of the
total number of clones in the library) encode ribosomal proteins. A
comparative analysis with a previous study, where clones from the same
library were selected using sera from patients with Chagas disease,
revealed that ribosomal proteins also represent the largest class of
antigen coding genes expressed in amastigotes (54% of all
immunoselected clones). However, although more than thirty classes of
ribosomal proteins were identified by EST analysis, the results of the
immunoscreening indicated that only a particular subset of them
contains major antigenic determinants recognized by antibodies from
Chagas disease patients