Analysis of renewal capacity of memory compartments and activated T cell replenishment of healed cutaneous leishmaniasis subjects.

<p>Renewal capacity of CD4⁺ (<b>A</b>) and CD8⁺ (<b>B</b>) T cells phenotipically characterized by central memory (Tcm, CD45RO⁺CCR7⁺) or effector memory (Tem, CD45RO⁺CCR7^-). The white box and whiskers represent those cells evaluated immediately after PBMC (<i>ex vivo</i>) and the gray boxes represent those cells after <i>in </i><i>vitro</i> stimuli with Lb-Ag. The central line represents median values. hCL= healed cutaneous leishmaniasis; HS= healthy subjects; *p<0.05, **p<0.01, ***p<0.001 (Kruskal-Wallis with post-test Dunns). Correlation analysis between the percentage of activated CD25⁺ (<b>C</b>, n=9) or CD69⁺ (<b>D</b>, n=12) in CD8⁺ T cells after <i>in </i><i>vitro</i> Lb-Ag stimuli and the percentage of effector memory (CD45RO⁺CCR7^-) CD8⁺ T cells after <i>in </i><i>vitro</i> Lb-Ag stimuli. Each point represents one subject. The graphs show the best fitted lines with 95% confidence intervals. r= correlation coefficient; p= significance level, (Spearman test).</p

Correlation analysis of clinical and/or immunological parameters from healed cutaneous leishmaniasis subjects.

<p>Correlation between the percentage of recently activated CD4⁺ (A, n=13) or CD8⁺ (B, n=14) T lymphocytes from PBMC after <i>in </i><i>vitro</i> Lb-Ag stimulation and the duration of clinical cure; Correlation between the percentage of activated CD4⁺ (C, n=8) or CD8⁺ (D, n=9) T lymphocytes and the concentration of IL-10 from cell culture supernatant; Each point represents one subject. The graphs show the best fitted lines with 95% confidence intervals. r= correlation coefficient; p= significance level, (Spearman test). </p