Experimental verification of theoretical approaches for radial gravity currents draining from an edge

We present an experimental study of inertial gravity currents (GCs) propagating in a cylindrical wedge under different drainage directions (inward/outward), lock-release (full/partial gatewidth) and geometry (annulus/full cylinder). We investigate the following combinations representative of operational conditions for dam-break flows: (i) inward drainage, annular reservoir, full gate; (ii) outward drainage, full reservoir, full gate; and (iii) outward drainage, full reservoir, partial gate. A single-layer shallow-water (SW) model is used for modelling the first two cases, while a box model interprets the third case; the results of these approximations are referred to as "theoretical". We performed a first series of experiments with water as ambient fluid and brine as intruding fluid, measuring the time evolution of the volume in the reservoir and the velocity profiles in several sections; in a second series, airwas the ambient andwaterwas the intruding fluid. Careful measurements, accompanied by comparisons with the theoretical predictions, were performed for the behaviour of the interface, radial velocity and, most important, the volume decay V(t)/V(0). In general, there is good agreement: the theoretical volume decay is more rapid than the measured one, but the discrepancies are a few percent and the agreement improves as the Reynolds number increases. Velocity measurements show a trend correctly reproduced by the SWmodel, although often a delay is observed and an over- or under-estimation of the peak values. Some experiments were conducted to verify the role of inconsistencies between experimental set-up and model assumptions, considering, for example, the presence or absence of a top lid, wedge angle much less than 2p, suppression of the viscous corner at the centre, reduction of disturbances in the dynamics of the ambient fluid: all these effects resulted in negligible impacts on the overall error. These experiments provide corroboration to the simple models used for capturing radial drainage flows, and also elucidate some effects (like oscillations of the radial flux) that are beyond the resolution of the models. This holds also for partial width lock-release, where axial symmetry is lost

Experimental verification of theoretical approaches for radial gravity currents draining from an edge

We present an experimental study of inertial gravity currents (GCs) propagating in a cylindrical wedge under different drainage directions (inward/outward), lock-release (full/partial gate width) and geometry (annulus/full cylinder). We investigate the following combinations representative of operational conditions for dam-break flows: (i) inward drainage, annular reservoir, full gate; (ii) outward drainage, full reservoir, full gate; and (iii) outward drainage, full reservoir, partial gate. A single-layer shallow-water (SW) model is used for modelling the first two cases, while a box model interprets the third case; the results of these approximations are referred to as “theoretical”. We performed a first series of experiments with water as ambient fluid and brine as intruding fluid, measuring the time evolution of the volume in the reservoir and the velocity profiles in several sections; in a second series, air was the ambient and water was the intruding fluid. Careful measurements, accompanied by comparisons with the theoretical predictions, were performed for the behaviour of the interface, radial velocity and, most important, the volume decay V(t) / V(0). In general, there is good agreement: the theoretical volume decay is more rapid than the measured one, but the discrepancies are a few percent and the agreement improves as the Reynolds number increases. Velocity measurements show a trend correctly reproduced by the SW model, although often a delay is observed and an over- or under-estimation of the peak values. Some experiments were conducted to verify the role of inconsistencies between experimental set-up and model assumptions, considering, for example, the presence or absence of a top lid, wedge angle much less than 2 π, suppression of the viscous corner at the centre, reduction of disturbances in the dynamics of the ambient fluid: all these effects resulted in negligible impacts on the overall error. These experiments provide corroboration to the simple models used for capturing radial drainage flows, and also elucidate some effects (like oscillations of the radial flux) that are beyond the resolution of the models. This holds also for partial width lock-release, where axial symmetry is lost

Mars Regolith Simulant Ameliorated by Compost as In Situ Cultivation Substrate Improves Lettuce Growth and Nutritional Aspects

Heavy payloads in future shuttle journeys to Mars present limiting factors, making self-sustenance essential for future colonies. Therefore, in situ resources utilization (ISRU) is the path to successful and feasible space voyages. This research frames the concept of planting leafy vegetables on Mars regolith simulant, ameliorating this substrate’s fertility by the addition of organic residues produced in situ. For this purpose, two butterhead lettuce (Lactuca sativa L. var. capitata) cultivars (green and red Salanova®) were chosen to be cultivated in four dierent mixtures of MMS-1 Mojave Mars simulant:compost (0:100, 30:70, 70:30 and 100:0; v:v) in a phytotron open gas exchange growth chamber. The impact of compost rate on both crop performance and the nutritive value of green- and red-pigmented cultivars was assessed. The 30:70 mixture proved to be optimal in terms of crop performance, photosynthetic activity, intrinsic water use eciency and quality traits of lettuce. In particular, red Salanova® showed the best performance in terms of these quality traits, registering 32% more phenolic content in comparison to 100% simulant. Nonetheless, the 70:30 mixture represents a more realistic scenario when taking into consideration the sustainable use of compost as a limited resource in space farming, while still accepting a slight significant decline in yield and quality in comparison to the 30:70 mixture

Current Acquaintance on Agronomic Biofortification to Modulate the Yield and Functional Value of Vegetable Crops: A Review

Fresh vegetables and fruits have always been the mainstays of good nutrition as providers of fiber, beneficial phytochemicals (such as vitamins and phenolic compounds), and minerals. Today and in the future, biofortification is a promising strategy to increase the concentration of these compounds. Considering the importance of minerals in human health, the enrichment of fresh produce for consumption has been considered through specific agronomic approaches. This review discusses, in detail, the latest findings on vegetable agronomic biofortification, aimed at increasing the concentration of crucial minerals, such as iron (Fe), zinc (Zn), iodine (I), selenium (Se), molybdenum (Mo), and silicon (Si), in edible portions, focusing on the direct and indirect effects of this strategy. Although agronomic biofortification is considered a feasible technique, the approach is complex due to the many interactions between the microelement bioavailability for both plants and consumers. Therefore, the effects of biofortification on human health and the influence of beneficial and antinutritional compounds were discussed in detail to analyze the advantages and disadvantages of this practice

Systematic inference and comparison of multi-scale chromatin sub-compartments connects spatial organization to cell phenotypes.

Chromatin compartmentalization reflects biological activity. However, inference of chromatin sub-compartments and compartment domains from chromosome conformation capture (Hi-C) experiments is limited by data resolution. As a result, these have been characterized only in a few cell types and systematic comparisons across multiple tissues and conditions are missing. Here, we present Calder, an algorithmic approach that enables the identification of multi-scale sub-compartments at variable data resolution. Calder allows to infer and compare chromatin sub-compartments and compartment domains in >100 cell lines. Our results reveal sub-compartments enriched for poised chromatin states and undergoing spatial repositioning during lineage differentiation and oncogenic transformation

Pan-Cancer Landscape of Aberrant DNA Methylation across Human Tumors.

The discovery of cancer-associated alterations has primarily focused on genetic variants. Nonetheless, altered epigenomes contribute to deregulate transcription and promote oncogenic pathways. Here, we designed an algorithmic approach (RESET) to identify aberrant DNA methylation and associated cis-transcriptional changes across >6,000 human tumors. Tumors exhibiting mutations of chromatin remodeling factors and Wnt signaling displayed DNA methylation instability, characterized by numerous hyper- and hypo-methylated loci. Most silenced and enhanced genes coalesced in specific pathways including apoptosis, DNA repair, and cell metabolism. Cancer-germline antigens (CG) were frequently epigenomically enhanced and their expression correlated with response to anti-PD-1, but not anti-CTLA4, in skin melanoma. Finally, we demonstrated the potential of our approach to explore DNA methylation changes in pediatric tumors, which frequently lack genetic drivers and exhibit epigenomic modifications. Our results provide a pan-cancer map of aberrant DNA methylation to inform functional and therapeutic studies

Development and Validation of a Reversed-Phase HPLC Method with UV Detection for the Determination of L-Dopa in Vicia faba L. Broad Beans

L-Dopa (LD), a substance used medically in the treatment of Parkinson's disease, is found in several natural products, such as Vicia faba L., also known as broad beans. Due to its low chemical stability, LD analysis in plant matrices requires an appropriate optimization of the chosen analytical method to obtain reliable results. This work proposes an HPLC-UV method, validated according to EURACHEM guidelines as regards linearity, limits of detection and quantification, precision, accuracy, and matrix effect. The LD extraction was studied by evaluating its aqueous stability over 3 months. The best chromatographic conditions were found by systematically testing several C-18 stationary phases and acidic mobile phases. In addition, the assessment of the best storage treatment of Vicia faba L. broad beans able to preserve a high LD content was performed. The best LD determination conditions include sun-drying storage, extraction in HCl 0.1 M, chromatographic separation with a Discovery C-18 column, 250 x 4.6 mm, 5 mu m particle size, and 99% formic acid 0.2% v/v and 1% methanol as the mobile phase. The optimized method proposed here overcomes the problems linked to LD stability and separation, thus contributing to the improvement of its analytical determination

Validation of an analytical method for nitrite and nitrate determination in meat foods for infants by ion chromatography with conductivity detection

Nitrate and nitrite as sodium or potassium salts are usually added to meat products to develop the characteristic flavor, to inhibit the growth of microorganisms (particularly Clostridium botulinum), and effectively control rancidity by inhibiting lipid oxidation. However, both nitrate and nitrite ions need to be monitored for ensuring the quality and safety of cured meats. In this work, for the first time the content of nitrite and nitrate ions in homogenized meat samples of baby foods was determined by a validated method based on ion chromatography (IC) coupled with conductivity detection. Recoveries of nitrate and nitrite ions in meat samples were not lower than 84 ± 6%. The detection limits of nitrate and nitrite were 0.08 mg L−1 and 0.13 mg L−1, respectively. Five commercial samples of homogenized meat, namely lamb, rabbit, chicken, veal, and beef, for infant feeding were investigated; while nitrite content was below the detection limit, nitrate ranged from 10.7 to 21.0 mg kg−1. The results indicated that nitrate contents were below the European (EU) fixed value of 200 mg kg−1, and an acceptable daily intake of 3.7 mg kg−1 was estimated

Gravity-driven flow of Herschel--Bulkley fluid in a fracture and in a 2D porous medium

New analytical models are introduced to describe the motion of a Herschel-Bulkley fluid slumping under gravity in a narrow fracture and in a porous medium. A useful self{similar solution can be derived for a fluid injection rate that scales as t; an expansion technique is adopted for a generic injection rate which is power{law in time. Experiments in a Hele-Shaw cell and in a narrow channel filled with glass ballotini confirm the theoretical model within the experimental uncertainty