Indagine sulla presenza di Salmonella spp. nelle spezie

In the last decades, food-borne infections and food poisoning, due to spices, have increased, in several European countries. The main cause is the improved consumption of ready-to-eat food and increased use of spices in food. A total of 158 samples of spices were analyzed from 2015 to 2019, they were collected in Palermo district, at commercialization or import phase. All samples were analysed for Salmonella spp. All microorganisms isolated were subjected to antibiotic resistance assay. The results of the analysis showed a satisfying hygienic quality of spices, only four samples (2,5%) of sesame showed the presence of Salmonella spp.. The Salmonella isolated strains showed sensitivity to all antibiotics tested except the ampicillin and gentamicin. The strain of S. Maastricht, instead, showed sensitivity to ampicillin and resistance to gentamicin, kanamycin, streptomycin and tetraciclin

Indagine sulla prevalenza di Vibrio spp. potenzialmente patogeni in prodotti ittici in Sicilia: nuovi dati

The aim of this study was to value the prevalence of enteropathogenic Vibrio spp. in fish products, collected in the last two years. A total of 100 samples (75 bivalve molluscs, 6 shrimps, 12 teleosts and 7 cephalopods) were analysed according to the ISO 21872-1:2017. Vibrio strains were characterised by molecular analysis (PCR). Enteropathogenic Vibrio spp were isolated in the 11% of fish products, particularly in shellfish. V. parahaemolyticus was identified in 4/75 (5.3%) and in 2/12 (16.7%) samples of bivalve molluscs from production areas and teleosts respectively. The isolates of V. parahaemolyticus were tested by PCR for the presence of specie- specific gene (toxR) and for the tdh and trh genes: 1 sample of teleosts (Sardina pilchardus) had the trh gene. V. vulnificus was isolated in 5/75 (6.7%) samples of bivalve molluscs (only in oysters). This is the first report of V. vulnificus identification in bivalve molluscs in our area

Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study

The aim of the study was to evaluate the occurrence of Arcobacter spp. in food samples collected from Sicilia region. A total of 91 food products of animal origin (41 meat, 17 fresh milk, 18 shellfish) and 15 samples of fresh vegetables, were examined by cultural method and confirmed by biochemical analysis and PCR methods. The detection of Arcobacter spp. was performed, after selective enrichment, on two selective agar plates: Arcobacter agar and mCCD (modified charcoal cefoperazone deoxycholate) agar supplemented with CAT (Cefoperazone, Amphotericin B and Teicoplanin). Arcobacter species were isolated using the membrane filtration technique. In 13 (14.3%) out of the 91 tested samples, the presence of Arcobacter spp. was found: the isolates were confirmed by multiplex PCR and identified as belonging to the species A. butzleri and A. cryaerophilus. The highest prevalence rate was observed in chicken meat (8.8%) followed by shellfish (3.3%). Negative results have been obtained for raw milks and vegetables samples. The preliminary study highlights the importance of this emerging pathogen and the need for further studies on its prevalence and distribution in different types of food for human consumption

Indagine sulla prevalenza di Vibrio spp. potenzialmente patogeni in prodotti ittici in Sicilia: nuovi dati

The aim of this study was to value the prevalence of enteropathogenic Vibrio spp. in fish products, collected in the last two years. A total of 100 samples (75 bivalve molluscs, 6 shrimps, 12 teleosts and 7 cephalopods) were analysed according to the ISO 21872-1:2017. Vibrio strains were characterised by molecular analysis (PCR). Enteropathogenic Vibrio spp were isolated in the 11% of fish products, particularly in shellfish. V. parahaemolyticus was identified in 4/75 (5.3%) and in 2/12 (16.7%) samples of bivalve molluscs from production areas and teleosts respectively. The isolates of V. parahaemolyticus were tested by PCR for the presence of specie- specific gene (toxR) and for the tdh and trh genes: 1 sample of teleosts (Sardina pilchardus) had the trh gene. V. vulnificus was isolated in 5/75 (6.7%) samples of bivalve molluscs (only in oysters). This is the first report of V. vulnificus identification in bivalve molluscs in our area

Prevalence and antimicrobial resistance profiles of <i>Salmonella</i> spp. in poultry meat

The spread of multidrug resistant (MDR) Salmonella strains, along the poultry supply chain, can represent a relevant threat to human health. This study aimed to evaluate the prevalence and antimicrobial resistance of Salmonella spp. isolated from poultry meat for human consumption. Between 2019 and 2021, 145 samples were analyzed according to ISO 6579-1:2017. The strains isolated were identified by using biochemical-enzymatic assays and serotyping, according to the Kauffmann-White-Le Minor scheme. The antibiotic susceptibility tests were determined using the Kirby-Bauer method. Forty Salmonella spp. strains were isolated and serotyping showed Salmonella Infantis to be predominant. 80% of the isolated strains were MDR and identified as S. Infantis. This study confirms the circulation of MDR Salmonella isolated from poultry meat and highlights the predominance of the S. Infantis serovar, which represents an emerging risk factor under the One Health holistic approach