Allelic diversity of S‑RNase alleles in diploid potato species

S-ribonucleases (S-RNases) control the pistil specificity of the self-incompatibility (SI) response in the genus Solanum and several other members of the Solanaceae. The nucleotide sequences of S-RNases corresponding to a large number of S-alleles or S-haplotypes have been characterised. However, surprisingly few S-RNase sequences are available for potato species. The identification of new S-alleles in diploid potato species is desirable as these stocks are important sources of traits such as biotic and abiotic resistance. S-RNase sequences are reported here from three distinct diploid types of potato: cultivated Solanum tuberosum Group Phureja, S. tuberosum Group Stenotomum, and the wild species Solanum okadae. Partial S-RNase sequences were obtained from pistil RNA by RT-PCR or 3’RACE (Rapid Amplification of cDNA Ends) using a degenerate primer. Full length sequences were obtained for two alleles by 5’RACE. Database searches with these sequences, identified sixteen S-RNases in total, all of which are novel. The sequence analysis revealed all the expected features of functional S-RNases. Phylogenetic analysis with selected published S-RNase and S-like-RNase sequences from the Solanaceae revealed extensive trans-generic evolution of the S-RNases and a clear distinction from S-like-RNases. Pollination tests were used to confirm the self-incompatibility status and cross-compatibility relationships of the S. okadae accessions. All the S. okadae accessions were found to be self-incompatible as expected with crosses amongst them exhibiting both cross-compatibility and semi-compatibility consistent with the S-genotypes determined from the S-RNase sequence data. The progeny analysis of four semi-compatible crosses examined by allele-specific PCR provided further confirmation that these are functional S-RNases

DNA methylation in diploid inbred lines of potatoes and its possible role in the regulation of heterosis

Self-incompatible diploid potatoes were altered to self-compatible ones by a function of S-locus inhibitor gene and continued selfing generated highly homozygous inbreds. In this study, this process was investigated for the status of DNA methylation by a simple method using genomic DNA digested by methylation-sensitive restriction enzymes prior to RAPD analysis. We detected 31 methylation-sensitive RAPD bands, of which 11 were newly appeared in the selfed progenies, and 6 of them stably inherited to subsequent generations. Aberrant segregations and paternal- or atavism-like transmission were also found. Segregating methylation-sensitive bands in initial populations became fixed in the advanced selfed progenies by 75.0–93.8%, of which 41.7% were fixed to all present and 58.3% to all absent. Because DNA methylation is generally recognized to suppress gene expression as regulatory factors, homozygosity/heterozygosity of methylated DNA may be involved in inbreeding depression/heterosis

Focal process of the great Chilean earthquake May 22, 1960

Long-period strain seismogram recorded at Pasadena is used to determine the focal process of the 1960 Chilean earthquake. Synthetic seismograms computed for various fault models are matched with the observed strain seismogram to determine the fault parameters. A low-angle (∼ 10°) thrust model with rupture length of 800 km and rupture velocity of 3.5 km/sec is consistent with the observed Rayleigh/Love wave ratio and the radiation asymmetry. A seismic moment of 2.7 · 10^(30) dyn · cm is obtained for the main shock. This value, together with the estimated fault area of 1.6 · 10^5 km^2, gives an average dislocation of 24 m. The strain seismogram clearly shows unusually long-period (300–600 sec) wave arriving at the P time of a large foreshock which occurred about 15 minutes before the main shock, suggesting a large slow deformation in the epicentral area prior to the major failure. A simple dislocation model shows that a dislocation of 30 m, having a time constant of 300–600 sec, over a fault plane of 800 × 200 km^2 is required to explain this precursory displacement. The entire focal process may be envisaged in terms of a large-scale deformation which started rather gradually and eventually triggered the foreshocks and the “main” shock. This mechanism may explain the large premonitory deformations documented, but not recorded instrumentally, for several Japanese earthquakes. The moments of the main shock and the precursor add to 6 · 10^(30) dyn · cm which is large enough to affect the earth's polar motion

Transparent contribution of memory

A multitude of research and commercial projects have proposed contributory systems that utilize wasted CPU cycles, idle memory and free disk space found on end-user machines. These applications include distributed computation such as signal processing and protein folding, peer-to-peer backup, and large-scale distributed storage. While users are generally willing to give up unused CPU cycles, the use of memory by contributory applications deters participation in such systems. Contributory applications pollute the machine’s memory, forcing user pages to be evicted to disk. This paging can disrupt user activity for seconds or even minutes. In this paper, we describe the design and implementation of an operating system mechanism to support transparent contribution of memory. A transparent memory manager (TMM) controls memory usage by contributory applications, ensuring that users will not notice an increase in the miss rate of their applications. TMM is able to protect user pages such that page miss overhead is limited to 1.7%, while donating hundreds of megabytes of memory.

TFS: A transparent file system for contributory storage

Contributory applications allow users to donate unused resources on their personal computers to a shared pool. Applications such a

Microearthquake measurements near South Sebec, Maine, 1989-1990

Maine Geological Survey, Open-File Report 91-5. Report on portable seismograph measurements of microearthquakes.https://digitalmaine.com/mgs_publications/1206/thumbnail.jp