Gel electrophoresis of ovine <i>SERPINA1</i> cDNA transcript variants.

<p>Molecular weight markers on the left (lane M), cDNA from mammary gland Sarda (lane 1) and Gentile di Puglia (lane 2) breeds, and cDNA from milk cells of Sarda (lane 3) and Gentile di Puglia (lane 4) breeds. The three identified splicing variants are indicated by arrows on right side of pictograph.</p

Alignment of the reactive center loop (RCL) region (25 aa) for AAT protein in different mammalian.

<p>RCL region is dotted underlined. The two amino acids important for the inhibitory AAT function are highlighted with a box.</p

Representative results of <i>SERPINA1</i> gene expression in different ovine tissues.

<p>A) Expression of <i>SERPINA1</i> transcript variants (indicated by arrows on right) and B) expression of ATPB5 control gene in various tissues. Lanes represent molecular weight marker (M) spleen (1), <i>semitendinosus</i> muscle (2), longissimus dorsi muscle (3), mammary gland (4), brain (5), cerebellum (6), rumen (7), bladder (8), adrenal (9), uterus (10) and liver (11)..</p