Inflammatory cytokines are not produced in response to DS in the cornea and conjunctiva of CCR6KO and CXCR3KO CD4⁺ T cell recipient mice.

<p>Wild-type (WT), CCR6KO, or CXCR3KO CD4⁺ T cells from NS or DS5 mice were adoptively transferred to T cell deficient RAG1KO mice. mRNA analysis for inflammatory cytokines and matrix metalloproteinases (MMP) was performed on the corneal (A) and conjunctival (B) tissues of CD4⁺ T cell RAG1KO recipient mice. Mean±SD of transcript levels of corneal and conjunctival tissues. Results represent the mean±SD expression of 2-3 animals per strain/time point per experiment in two independent experiments for a total of 5-6 mice. *, P<0.05, **, P<0.001, ***, P<0.0001.</p

CCR6KO and CXCR3KO mice are resistant to dry eye disease.

<p><b>A.</b> Corneal permeability - Mean±SD of Oregon green dextran (OGD) permeability into the corneal epithelium of wild-type (WT), CCR6KO and CXCR3KO mice without (NS) or with 5 days of desiccating stress (DS5). OGD permeability was measured in five to seven mice per strain/time point/experiment. The graph represents the combined results of two independent experiments with a total of 10-14 mice per experimental group. <b>B.</b> Representative pictures of OGD staining of each strain. <b>C.</b> CD4⁺ T cell density – Mean±SD of the number of CD4⁺ cells per 100 µm of conjunctival epithelium of WT, CCR6KO, and CXCR3KO mice without (NS) or with 5 (DS5) or 10 (DS10) days of desiccating stress. <b>D.</b> Representative pictures of CD4⁺ T cell infiltration in each strain. * - indicates goblet cells; ↓ - indicates CD4⁺ T cells; CJ – conjunctiva; K – cornea. <b>E.</b> Goblet cell density - Mean±SD of the number of goblet cells per mm of conjunctival epithelium of WT, CCR6KO and CXCR3KO mice without (NS) or with 5 (DS5) or 10 (DS10) days of desiccating stress. Three sections from three animals were examined (N = 9) for each time point/mouse strain for both CD4⁺ T cell and goblet cell density. <b>F.</b> Representative pictures of PAS staining of each strain.</p

Th1 and Th17 associated cytokines are produced in the cervical lymph node but not the ocular surface.

<p><b>A.</b> Gene expression of IFN-γ, IL-17A, and IL-13 in the superficial cervical lymph node (CLN) of wild-type C57BL/6, CCR6KO, and CXCR3KO exposed to DS for 5 days (DS5) or not exposed (NS). <b>B.</b> Gene expression of IFN-γ, IL-17A, and IL-13 the ocular surface of wild-type C57BL/6, CCR6KO, and CXCR3KO exposed to DS for 5 days (DS5) or not exposed (NS). Results represent the mean±SD expression 2-3 animals per strain per time point in two independent experiments with a total of five mice. *, P<0.05, **, P<0.01.</p

Increased expression of CCR6⁺ and CXCR3⁺ cells in the cervical lymph node and at the ocular surface in response the desiccating stress.

<p><b>A.</b> The percentage of CCR6⁺CD4⁺ T cells in the superficial cervical lymph node (CLN) in mice exposed to no stress (NS) or five (DS5) days of desiccating stress (DS) was determined by flow cytometry. The results represent mean±SD of five samples per time point for a total of ten samples (N = 10) in two independent experiments. <b>B.</b> The percentage of CCR6⁺CD4⁺ T cells in the ocular surface (OS). The results represent mean±SD of five samples per time point for a total of ten samples (N = 10) in two independent experiments. <b>C.</b> The percentage of CXCR3⁺CD4⁺ T cells at the CLN. The results represent mean±SD of five samples per time point for a total of ten samples (N = 10) in two independent experiments. <b>D.</b> The percentage of CXCR3⁺CD4⁺ T cells at the OS. The results represent mean±SD of five samples per time point for a total of ten samples (N = 10) in two independent experiments. <b>E.</b> A representative dot plot of the percentage of CD4⁺ cells that are CCR6⁺ or CXCR3⁺ in the CLN.</p

Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions-2

<p><b>Copyright information:</b></p><p>Taken from "Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions"</p><p></p><p>Molecular Vision 2007;13():1934-1941.</p><p>Published online 16 Oct 2007</p><p>PMCID:PMC2185513.</p><p></p

Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions-0

<p><b>Copyright information:</b></p><p>Taken from "Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions"</p><p></p><p>Molecular Vision 2007;13():1934-1941.</p><p>Published online 16 Oct 2007</p><p>PMCID:PMC2185513.</p><p></p

Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions-1

<p><b>Copyright information:</b></p><p>Taken from "Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions"</p><p></p><p>Molecular Vision 2007;13():1934-1941.</p><p>Published online 16 Oct 2007</p><p>PMCID:PMC2185513.</p><p></p