Iatrogenic Cushing’s Syndrome with Subsequent Adrenal Insufficiency in a Patient with Psoriasis Vulgaris Using Topical Steroids

Iatrogenic Cushing’s syndrome (ICS) is usually related to prolonged and/or high-dose oral or parenteral steroid use. Psoriasis vulgaris (PV) is chronic inflammatory disease and characterized by periods of attack and remission. Topical steroid (TS) is the first choice of treatment for localized and mild PV. The development of systemic side effects of the steroids is usually not observed after TS application. But the risk of developing ICS still exists. In the literature, there are a few adult cases who developed ICS and subsequent adrenal insufficiency associated with TS. In this article, a male patient with PV developing ICS and secondary adrenal insufficiency after treatment of TS for 12 years is presented

Relationship between Lymphocytes, IL2 and the Hormones E2, LH, PRG and FSH in Menopausal and Postmenopausal Women

Proble

Which is Better: Stainless Steel or Titanium Alloy?

WOS: 000437251200018AIM: To investigate immunologic reactions after implantation of stainless steel (SS) alloy and titanium (Ti) alloy in a rat model. Macrophage and cytokine responses have been reported after in vivo and in vitro application of different biomaterials. MATERIAL and METHODS: Wistar albino rats were used. After an exploration of the thoracolumbar paravertebral muscle tissue of the subjects, Group I underwent sham surgery, and Groups II and III underwent implantation of Ti alloy and SS alloy rods respectively. The CD4, CD8, CD25 (IL-2R) (lymphocyte and CD4 gate), CD4+CD8+ and CD4+CD25+Foxp3+ (Tregs), IL-4, IL-10, IL-6, IL-17A, TGF-beta,TNF-alpha in the blood were analyzed. RESULTS: CD4, CD25 (IL-2R), CD4+CD8+ and Tregs levels were lower in Group III compared to Group I (sham) and Group II. IL-6, IL-17A, TGF-beta and TNF-alpha levels in Group III showed a significant increase on all days in comparison with Group I and Group II. IL-4 and IL-10 levels were lower in Group III than those in Group II; and a significant decrease was observed in the IL-10 level. There was a reduction in IL-6 and IL-17A levels in Group II as opposed to Group I. CONCLUSION: As opposed to SS alloy, Ti alloy suppresses the development of inflammation by inhibiting the pro-inflammatory response; strengthens the humoral immune system by intensifying the antibody-dependent immune response; triggers the development of immune tolerance by regulating the immune response; and activates the mechanism that prevents immune response-related damage from occurring

K562 cells display different vulnerability to H2O2 induced oxidative stress in differing cell cycle phases

Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temporary arrest may be a period of time given to the cells to repair the DNA damage before entering the cycle again and completing mitosis. In order to determine the effects of oxidative stress on several cell cycle phases, human erytroleukemia cell line (K562) was synchronized with mimosine and genistein, and cell cycle analysis carried out. Synchronized cells were exposed to oxidative stress with hydrogen peroxide (H2O2) at several concentrations and different times. Changes on mitochondria membrane potential (m) of K562 cells were analyzed in G(1), S, and G(2)/M using Rhodamine 123 (Rho 123). To determine apoptosis and necrosis, stressed cells were stained with Annexin V (AnnV) and propidium iodide (PI) for flow cytometry. Changes were observed in the m of synchronized and asynchronized cells that were exposed to oxidative stress. Synchronized cells in S phase proved resistant to the effects of oxidative stress and synchronized cells at G(2)/M phase were sensitive to the effects of H2O2-induced oxidative stress at 500M and above

beta-carotene treatment alters the cellular death process in oxidative stress-induced K562 cells

Oxidizing agents (e.g., H2O2) cause structural and functional disruptions of molecules by affecting lipids, proteins, and nucleic acids. As a result, cellular mechanisms related to disrupted macro molecules are affected and cell death is induced. Oxidative damage can be prevented at a certain point by antioxidants or the damage can be reversed. In this work, we studied the cellular response against oxidative stress induced by H2O2 and antioxidant-oxidant (-carotene-H2O2) interactions in terms of time, concentration, and treatment method (pre-, co-, and post) in K562 cells. We showed that co- or post-treatment with-carotene did not protect cells from the damage of oxidative stress furthermore co- and post-beta-carotene-treated oxidative stress induced cells showed similar results with only H2O2 treated cells. However, -carotene pre-treatment prevented oxidative damage induced by H2O2 at concentrations lower than 1,000 mu M compared with only H2O2-treated and co- and post-beta-carotene-treated oxidative stress-induced cells in terms of studied cellular parameters (mitochondrial membrane potential [Delta psi(m)], cell cycle and apoptosis). Prevention effect of beta-carotene pre-treatment was lost at concentrations higher than 1,000 mu M H2O2 (2-10mM). These findings suggest that beta-carotene pre-treatment alters the effects of oxidative damage induced by H2O2 and cell death processes in K562 cells

DOCK8 Levels in Patients with Hyperimmunoglobulin E Sendrome: Detection by Flow Cytometry

Objectives: In this study, we report the findings of DOCK8 detected by flow cytometry in patients diagnosed with hyperimmunoglobulin E syndrome (HIES)

Effect of the Achillea wilhelmsii extract intake upon blood lipid profile, haematologic and immunologic parameters in the rat

Achillea species are widely used in folk medicine as antispasmodic, choloretic, antiphlogistic, antifungal and antibacterial agents, but little is known about their effects on the immune system and blood lipid profile. In this study, effects of the Achillea wilhelmsii methanolic extracts (ME) upon the lipid, haematological parameters and immune system were examined. ME was given at dose 75 mg/kg/day for 30 days to Sprague-Dawley rats fed with high-fat diet. Haematology, flow cytometry, clinical, biochemical and histopathological examinations were performed in the sacrificed animals. Significant decreases in triglyceride and very low density lipoprotein cholesterol, and increases in CD4 expression in lymphocytes and monocytes were observed. Assessment of alanin aminotransferase, aspartat aminotransferase activities, histopathological examinations and proinflammatory cytokine levels (IL-1 beta, IL-6, IL-10, TNF-alpha) showed neither liver damage nor inflammation induction. Beside the improvements in lipid profile by ME treatment without any liver damage and inflammation, the increase of CD4 expression both on lymphocyte and monocyte populations need further investigation

Nicotine reduces effectiveness of doxorubicin chemotherapy and promotes CD44<sup>+</sup>CD24<sup>-</sup> cancer stem cells in MCF-7 cell populations.

Breast cancer is the most common type of cancer in females and the second most common cause of cancer mortality after lung cancer. Cancer stem cells represent a novel approach to target cancer and reduce cancer recurrence and metastasis. Many patients with breast cancer continue to smoke after receiving their diagnosis. Nicotine is a key factor in tobacco addiction and also changes some cellular functions, such as activation of mitogenic pathways, angiogenesis and cell proliferation. In the present study, the impact of nicotine was assessed in a population of MCF-7 human breast cancer cells. Cluster of differentiation (CD)44(+)CD24(-) cancer stem cell population of MCF-7 cells were evaluated using flow cytometry and scanning electron microscopy. Chemoresistance effects of nicotine were demonstrated in these cells. These findings demonstrated harmful effects of nicotine following metastasis of cancer, owing to the chemoresistance produced through uninterrupted smoking, which may impact the effectiveness of treatment