Association of Cytokine Gene Polymorphisms (IL6, IL10, TNF-alpha, TGF-beta and IFN-gamma) and Graves' Disease in Turkish Population

Introduction: Cytokines play a crucial role in the pathogenesis of autoimmune thyroid disease, and recent studies have demonstrated an association between cytokine gene polymorphisms and Graves' Disease (GD) in different ethnic groups. The aim of the present study was to investigate the relationship of interleukin-6 (IL-6), IL-10, tumor necrosis factor-alpha (TNF-alpha), transforming growth factor-beta (TGF-beta), and interferon-gamma (INF-gamma) gene polymorphisms in the development of GD in Turkish population

Cytokine Polymorphism and HLA Genotyping in Patients with Temporal Lobe Epilepsy Related to Hippocampal Sclerosis

Objective: Hippocampal sclerosis (HS) is the most common pathological substrate associated with mesial temporal lobe epilepsy (MTLE), where inflammatory processes are known to play an increasingly important role in the pathogenesis. To further investigate the role of the immune system, both cytokine gene polymorphisms and human leukocyte antigen (HLA) genotyping in patients with MTLE-HS were investigated

Characterization of Minor HA-1 in Patients Who Underwent Living Donor Kidney Transplantation

Objectives: The importance of the Human Leukocyte Antigens (HLA) matching is well known in renal transplantation that is one of the best treatment options for end-stage solid organ deficiency. Human Leukocyte Antigens matching between recipient and donor has an influence on graft survey after the renal transplantation. However allogeneic renal transplants between HLA identical siblings might be ended with rejection. Minor histocompatibility antigen (mHag) HA-1 is a nine-amino acid peptide encoded by a diallelic gene on human chromosome 19. mHags have low polymorphisms. Minor histocompatibility antigens are likely to function as potantial risks for graft rejection of HLA-matched solid organ transplantation. In our study, we aimed to investigate the effect of minor HA-1 mismatch on kidney transplant survey

Undetected HLA-A,B Antigens By Serological Method And Application Of Molecular Methods

Since human leucocyte compatibility has a crucial effect in renal transplantations and there is the need for full-match HLA compatibility between the donor and the recipient for successful bone marrow transplantations. It is clearly evident that HLA system plays a major role in kidney and hone marrow transplantations. Hence, accurate and reliable identification of these antigens is very important. We perform CDC assay for the typing of HLA-A,-B in our EFT accredited laboratories. In case of homozygosity or detection of an ambigious antigen, molecular methods are performed in addition to CDC. The purpose of our study was to demonstrate the comparative results of two methods concerning the cases in which molecular typing was needed in addition to CDC and to list the antigens that could not he identified by CDC hut molecular typing. The study group included 1567 individuals consisting of patients with chronic renal deficiencies (n=646), hematological malignancies (n=646) and their donors (n=275). Samples were typed by CDC and PCR-SSP/SSO methods for HLA-A,-B. The study group was divided into 5 groups as cases with single HLA-A and/or-B and with ambigious HLA-A and/or-B. By molecular methods, 2138 tests were performed. The concordance between CDC assay and molecular methods was 8.8% for HLA-A and 14.2% for HLA-B. The most frequent antigens which could not be identified by CDC but molecular methods were HLA-A32, B15 (7.3, 15.8 %). We believe that performing molecular tissue typing methods at least particularly to patient samples will increase the transplantation success

MHC Class I related chain A (MICA), Human Leukocyte Antigen (HLA)-DRB1, HLA-DQB1 genotypes in Turkish patients with ulcerative colitis

Objectives: We aimed to determine Human LeukocyteAntigen (HLA)-DRB1, DQB1, and MHC Class I related chainA (MICA) genotypes in patients with ulcerative colitis.Methods: HLA-DRB1, HLA-DQB1, MICA genotyping ofpatient (n:85) and controls (n:100) were performed by PCRSSOLuminex (One Lambda genotyping kit).Results: We found significantly higher DRB1*01 (p:0.022,OR:0.23, CI:0.06–0.8) and MICA*0002/20/55 (p:0.03,OR:0.53, CI:0.29–0.93) alleles in control group whereasDRB1*14 (p:0.04, OR:2.25, CI:1–5.08), DRB1*15 (p:<0.0001,OR:4.54, CI:2.09–9.88) and MICA*0004 (p:0.01, OR:2.84,CI:1.2–6.7) alleles were higher in patient group.Conclusions: The present study will inform the MICAand HLA genotypes about the protective (DRB1*01,MICA*0002/20/55) or susceptible (DRB1*14, DRB1*15,MICA*0004) alleles of the disease and helps the literatureon Turkish patients with ulcerative colitis