1 research outputs found
Overexpression of Malonyl-CoA: ACP Transacylase in <i>Schizochytrium</i> sp. to Improve Polyunsaturated Fatty Acid Production
Polyunsaturated fatty acids (PUFAs)
have been widely applied in
the food and medical industry. In this study, malonyl-CoA: ACP transacylase
(MAT) was overexpressed through homologous recombination to improve
PUFA production in <i>Schizochytrium</i>. The results showed
that the lipid and PUFA concentration were increased by 10.1 and 24.5%
with MAT overexpression, respectively. Metabolomics analysis revealed
that the intracellular tricarboxylic acid cycle was weakened and glucose
absorption was accelerated in the engineered strain. In the mevalonate
pathway, intracellular carotene content was decreased, and the carbon
flux was then redirected toward PUFA synthesis. Furthermore, a glucose
fed-batch fermentation was finally performed with the engineered <i>Schizochytrium</i>. The total lipid yield was further increased
to 110.5 g/L, 39.6% higher than the wild strain. Docosahexaenoic acid
and eicosapentaenoic acid yield were enhanced to 47.39 g/L and 1.65
g/L with an increase of 81.5 and 172.5%, respectively. This study
provided an effective metabolic engineering strategy for industrial
PUFA production