6 research outputs found
Study of the biocompatibility of resorbable mesh prostheses for plastic surgery repair of abdominal wall and small pelvic floor defects
Subject Comparison of physico-chemical and biomedical properties of test specimens of mesh implants based on polydioxanone and polyglycolide, looking for application in gynecology and general surgery. Material and methods. In vitro evaluation of cytotoxicity of samples was performed using the MTT assay. Assessment of in vivo biocompatibility prosthesis was performed on outbred rats by simulating polnosloynogo defect of the anterior abdominal wall, which is sutured to the edges of polydioxanone and polyglycolic prosthesis.As a comparison group using the prosthesis on the basis of detsellyulyarizirovannoy dermis (Permacol). The animals were taken from the experiment 3, 10, 30 and 60 days after surgery. Was conducted macroscopic, tenziometricheskoe, morphometric, immunohistochemical and western blot studies. Results. The test prostheses do not possess cytotoxic properties, but in an in vivo experiment showed different intensity of adhesions, the speed and efficiency of integration with the surrounding tissues, preservation of the biomechanical properties of the severity of the inflammatory response. Conclusion. For some indicators of biocompatibility and efficacy of polydioxanone prosthesis was significantly better than prosthesis from polyglycolide and detsellyulyarizirovannoy dermis. Β© Bionika Media Ltd
ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π±ΠΈΠΎΡΠΎΠ²ΠΌΠ΅ΡΡΠΈΠΌΠΎΡΡΠΈ ΡΠ΅Π·ΠΎΡΠ±ΠΈΡΡΠ΅ΠΌΡΡ ΡΠ΅ΡΡΠ°ΡΡΡ ΠΏΡΠΎΡΠ΅Π·ΠΎΠ² Π΄Π»Ρ ΠΏΠ»Π°ΡΡΠΈΠΊΠΈ Π΄Π΅ΡΠ΅ΠΊΡΠΎΠ² Π±ΡΡΡΠ½ΠΎΠΉ ΡΡΠ΅Π½ΠΊΠΈ ΠΈ Π΄Π½Π° ΠΌΠ°Π»ΠΎΠ³ΠΎ ΡΠ°Π·Π°
Subject Comparison of physico-chemical and biomedical properties of test specimens of mesh implants based on polydioxanone and polyglycolide, looking for application in gynecology and general surgery. Material and methods. In vitro evaluation of cytotoxicity of samples was performed using the MTT assay. Assessment of in vivo biocompatibility prosthesis was performed on outbred rats by simulating polnosloynogo defect of the anterior abdominal wall, which is sutured to the edges of polydioxanone and polyglycolic prosthesis.As a comparison group using the prosthesis on the basis of detsellyulyarizirovannoy dermis (Permacol). The animals were taken from the experiment 3, 10, 30 and 60 days after surgery. Was conducted macroscopic, tenziometricheskoe, morphometric, immunohistochemical and western blot studies. Results. The test prostheses do not possess cytotoxic properties, but in an in vivo experiment showed different intensity of adhesions, the speed and efficiency of integration with the surrounding tissues, preservation of the biomechanical properties of the severity of the inflammatory response. Conclusion. For some indicators of biocompatibility and efficacy of polydioxanone prosthesis was significantly better than prosthesis from polyglycolide and detsellyulyarizirovannoy dermis. Β© Bionika Media Ltd.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. Π‘ΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΠΌΠ΅Π΄ΠΈΠΊΠΎ-Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΡΠ΅ΡΡΠ°ΡΡΡ
ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΎΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠ΄ΠΈΠΎΠΊΡΠ°Π½ΠΎΠ½Π° ΠΈ ΠΏΠΎΠ»ΠΈΠ³Π»ΠΈΠΊΠΎΠ»ΠΈΠ΄Π°, ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΡ
Π΄Π»Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² Π³ΠΈΠ½Π΅ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ ΠΎΠ±ΡΠ΅ΠΉ Ρ
ΠΈΡΡΡΠ³ΠΈΠΈ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠ΅Π½ΠΊΡ ΡΠΈΡΠΎΡΠΎΠΊΡΠΈΡΠ½ΠΎΡΡΠΈ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² in vitro ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΠ’Π’-ΡΠ΅ΡΡΠ°. ΠΡΠ΅Π½ΠΊΡ Π±ΠΈΠΎΡΠΎΠ²ΠΌΠ΅ΡΡΠΈΠΌΠΎΡΡΠΈ ΠΏΡΠΎΡΠ΅Π·ΠΎΠ² in vivo ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° Π±Π΅ΡΠΏΠΎΡΠΎΠ΄Π½ΡΡ
ΠΊΡΡΡΠ°Ρ
ΠΏΡΡΠ΅ΠΌ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠ»Π½ΠΎΡΠ»ΠΎΠΉΠ½ΠΎΠ³ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠ° ΠΏΠ΅ΡΠ΅Π΄Π½Π΅ΠΉ Π±ΡΡΡΠ½ΠΎΠΉ ΡΡΠ΅Π½ΠΊΠΈ, ΠΊ ΠΊΡΠ°ΡΠΌ ΠΊΠΎΡΠΎΡΠΎΠ³ΠΎ ΠΏΠΎΠ΄ΡΠΈΠ²Π°Π»ΠΈ ΠΏΠΎΠ»ΠΈΠ΄ΠΈΠΎΠΊΡΠ°Π½ΠΎΠ½ΠΎΠ²ΡΠΉ ΠΈΠ»ΠΈ ΠΏΠΎΠ»ΠΈΠ³Π»ΠΈΠΊΠΎΠ»ΠΈΠ΄Π½ΡΠΉ ΠΏΡΠΎΡΠ΅Π·. Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π³ΡΡΠΏΠΏΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΏΡΠΎΡΠ΅Π· Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΄Π΅ΡΠ΅Π»Π»ΡΠ»ΡΡΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π΄Π΅ΡΠΌΡ (Permacol). ΠΠΈΠ²ΠΎΡΠ½ΡΡ
Π²ΡΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΈΠ· ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ° Π½Π° 3, 10, 30 ΠΈ 60-Π΅ ΡΡΡΠΊΠΈ ΠΏΠΎΡΠ»Π΅ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ. ΠΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ°ΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡΠ΅ΡΠΊΠΎΠ΅, ΡΠ΅Π½Π·ΠΈΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ΅, ΠΌΠΎΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ΅, ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈ Π²Π΅ΡΡΠ΅ΡΠ½-Π±Π»ΠΎΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΠ΅ ΠΏΡΠΎΡΠ΅Π·Ρ Π½Π΅ ΠΎΠ±Π»Π°Π΄Π°Π»ΠΈ ΡΠΈΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ, Π½ΠΎ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ in vivo ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ ΡΠ°Π·Π½ΡΡ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠΏΠ°Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°, ΡΠΊΠΎΡΠΎΡΡΡ ΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠΈ Ρ ΠΎΠΊΡΡΠΆΠ°ΡΡΠΈΠΌΠΈ ΡΠΊΠ°Π½ΡΠΌΠΈ, ΡΠΎΡ
ΡΠ°Π½Π½ΠΎΡΡΡ Π±ΠΈΠΎΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ², ΡΡΠ΅ΠΏΠ΅Π½Ρ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎ ΡΡΠ΄Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Π±ΠΈΠΎΡΠΎΠ²ΠΌΠ΅ΡΡΠΈΠΌΠΎΡΡΠΈ ΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΡΠΎΡΠ΅Π· ΠΈΠ· ΠΏΠΎΠ»ΠΈΠ΄ΠΈΠΎΠΊΡΠ°Π½ΠΎΠ½Π° ΠΎΠΊΠ°Π·Π°Π»ΡΡ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π»ΡΡΡΠ΅, ΡΠ΅ΠΌ ΠΏΡΠΎΡΠ΅Π· ΠΈΠ· ΠΏΠΎΠ»ΠΈΠ³Π»ΠΈΠΊΠΎΠ»ΠΈΠ΄Π° ΠΈ Π΄Π΅ΡΠ΅Π»Π»ΡΠ»ΡΡΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π΄Π΅ΡΠΌΡ
Development of the bioartificial vaginal wall: An in vitro stage
Objective. To obtain a tissue-engineered construct based on a nonwoven polycaprolactone carrier, human epithelial and stromal cells to make an artificial vagina. Material and methods. Fibrous material based on polycaprolactone was obtained by an electrospinning technique. A capillary method was used to colonize the full-thickness tissue-engineering design by stromal cells and a static method was employed to colonize the surface layer of the construct by vaginal epithelial cells. Results. The vaginal epithelial cells expressed EpSAM and p63; the stromal cells did vimentin and smooth muscle actin. After colonization, the stromal cells were evenly distributed in a 1.5-mm thick matrix; the epithelial cells were arranged in a dense layer on the inner surface of the construct, sinking to a depth of 88.9Β±32.5 ΞΌm. Conclusion. The tissue-engineered construct obtained is similar to the native vagina in architectonics and cell composition. Β© Bionika Media Ltd
Π ΠΠΠ ΠΠΠΠ’ΠΠ ΠΠΠΠΠ Π’ΠΠ€ΠΠ¦ΠΠΠΠ¬ΠΠΠ Π‘Π’ΠΠΠΠ ΠΠΠΠΠΠΠΠ©Π: IN VITRO Π‘Π’ΠΠΠΠ―
Objective. To obtain a tissue-engineered construct based on a nonwoven polycaprolactone carrier, human epithelial and stromal cells to make an artificial vagina. Material and methods. Fibrous material based on polycaprolactone was obtained by an electrospinning technique. A capillary method was used to colonize the full-thickness tissue-engineering design by stromal cells and a static method was employed to colonize the surface layer of the construct by vaginal epithelial cells. Results. The vaginal epithelial cells expressed EpSAM and p63; the stromal cells did vimentin and smooth muscle actin. After colonization, the stromal cells were evenly distributed in a 1.5-mm thick matrix; the epithelial cells were arranged in a dense layer on the inner surface of the construct, sinking to a depth of 88.9Β±32.5 ΞΌm. Conclusion. The tissue-engineered construct obtained is similar to the native vagina in architectonics and cell composition. Β© Bionika Media Ltd.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π½Π΅ΡΠΊΠ°Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½ΠΎΠ²ΠΎΠ³ΠΎ Π½ΠΎΡΠΈΡΠ΅Π»Ρ, ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π΄Π»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΈΡΠΊΡΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π²Π»Π°Π³Π°Π»ΠΈΡΠ°. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΎΠ»ΠΎΠΊΠ½ΠΈΡΡΡΠΉ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π» Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π° ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ. ΠΠ»Ρ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π²ΡΠ΅ΠΉ ΡΠΎΠ»ΡΠΈ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄, Π΄Π»Ρ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π²Π»Π°Π³Π°Π»ΠΈΡΠ° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΠΎΠ³ΠΎ ΡΠ»ΠΎΡ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ - ΡΡΠ°ΡΠΈΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ Π²Π»Π°Π³Π°Π»ΠΈΡΠ° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ EpCAM ΠΈ p63, ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π²ΠΈΠΌΠ΅Π½ΡΠΈΠ½ ΠΈ Π³Π»Π°Π΄ΠΊΠΎΠΌΡΡΠ΅ΡΠ½ΡΠΉ Π°ΠΊΡΠΈΠ½. ΠΠΎΡΠ»Π΅ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ Π±ΡΠ»ΠΈ ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ Π² ΠΌΠ°ΡΡΠΈΠΊΡΠ΅ ΡΠΎΠ»ΡΠΈΠ½ΠΎΠΉ 1,5 ΠΌΠΌ; ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΡΠ°ΡΠΏΠΎΠ»Π°Π³Π°Π»ΠΈΡΡ ΠΏΠ»ΠΎΡΠ½ΡΠΌ ΠΏΠ»Π°ΡΡΠΎΠΌ Π½Π° Π²Π½ΡΡΡΠ΅Π½Π½Π΅ΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ, ΠΏΠΎΠ³ΡΡΠΆΠ°ΡΡΡ Π½Π° Π³Π»ΡΠ±ΠΈΠ½Ρ 88,9Β±32,5 ΠΌΠΊΠΌ. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ»ΡΡΠ΅Π½Π½Π°Ρ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½Π°Ρ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΡ Π±Π»ΠΈΠ·ΠΊΠ° ΠΏΠΎ ΡΠ²ΠΎΠ΅ΠΉ Π°ΡΡ
ΠΈΡΠ΅ΠΊΡΠΎΠ½ΠΈΠΊΠ΅ ΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΌΡ ΡΠΎΡΡΠ°Π²Ρ Π½Π°ΡΠΈΠ²Π½ΠΎΠΌΡ Π²Π»Π°Π³Π°Π»ΠΈΡΡ
Nonwoven polycaprolactone scaffolds for tissue engineering: The choice of the structure and the method of cell seeding
Nonwoven polycaprolactone materials produced by electrospinning are perspective internal prosthetic implants. Seeding these implants with multipotent mesenchymal stromal cells stimulates the replacement of the prosthesis with recipient's own connective tissue. Electrospinning method was used for producing polycaprolactone matrices differing in thickness, pore diameter, fiber size, and biomechanical properties. Labeled cells were seeded on scaffolds in three ways: (1) static, (2) dynamic, and (3) directed flow of the cell suspension generated by capillary action. Cell distribution on the surface and the interior of the scaffolds was studied; the metabolic activity of cells was measured by MTT assay. Static seeding method yielded fully confluence of cells covered the entire scaffold surface, but the cells were located primarily in the upper third of the matrix. Dynamic method proved to be effective only for scaffolds of thickness greater than 500 microns, irrespective of the pore diameter. The third method was effective only for scaffolds with the pore diameter of 20-30 microns, regardless of the material thickness. Resorbable nonwoven polycaprolactone electrospun materials have appropriate biomechanical properties and similar to native tissue matrix structures for internal prosthesis. The choice of the most effective cell seeding method depends on the spatial characteristics - the material thickness, pore diameter, and fibers size, which are determined by the electrospinning conditions
ΠΠ΅ΡΠΊΠ°Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π° Π΄Π»Ρ ΡΠΊΠ°Π½Π΅Π²ΠΎΠΉ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠΈΠΈ: Π²ΡΠ±ΠΎΡ ΡΡΡΡΠΊΡΡΡΡ ΠΈ ΡΠΏΠΎΡΠΎΠ±Π° Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ
Nonwoven polycaprolactone materials produced by electrospinning are perspective internal prosthetic implants. Seeding these implants with multipotent mesenchymal stromal cells stimulates the replacement of the prosthesis with recipient's own connective tissue. Electrospinning method was used for producing polycaprolactone matrices differing in thickness, pore diameter, fiber size, and biomechanical properties. Labeled cells were seeded on scaffolds in three ways: (1) static, (2) dynamic, and (3) directed flow of the cell suspension generated by capillary action. Cell distribution on the surface and the interior of the scaffolds was studied; the metabolic activity of cells was measured by MTT assay. Static seeding method yielded fully confluence of cells covered the entire scaffold surface, but the cells were located primarily in the upper third of the matrix. Dynamic method proved to be effective only for scaffolds of thickness greater than 500 microns, irrespective of the pore diameter. The third method was effective only for scaffolds with the pore diameter of 20-30 microns, regardless of the material thickness. Resorbable nonwoven polycaprolactone electrospun materials have appropriate biomechanical properties and similar to native tissue matrix structures for internal prosthesis. The choice of the most effective cell seeding method depends on the spatial characteristics - the material thickness, pore diameter, and fibers size, which are determined by the electrospinning conditions.ΠΠ΅ΡΠΊΠ°Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π°, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ, ΡΠ²Π»ΡΡΡΡΡ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠ°ΠΌΠΈ Π΄Π»Ρ ΡΠ½Π΄ΠΎΠΏΡΠΎΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΠ°ΡΠ΅Π»Π΅Π½ΠΈΠ΅ ΡΠ°ΠΊΠΈΡ
ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΎΠ² ΠΌΡΠ»ΡΡΠΈΠΏΠΎΡΠ΅Π½ΡΠ½ΡΠΌΠΈ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ Π·Π°ΠΌΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΡΠ΅Π·Π° ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΎΠ΅Π΄ΠΈΠ½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΡΡ ΡΠ΅ΡΠΈΠΏΠΈΠ΅Π½ΡΠ°. Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²Π»ΡΠ»ΠΎΡΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΡΠ΅Ρ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π½Π΅ΡΠΊΠ°Π½ΡΡ
Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π°, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΡ
ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ°ΠΌΠΈ. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΡΡΠΈ ΠΎΠ±ΡΠ°Π·ΡΠ° ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½ΠΎΠ²ΡΡ
ΠΌΠ°ΡΡΠΈΡ, ΠΎΡΠ»ΠΈΡΠ°ΡΡΠΈΡ
ΡΡ ΡΠΎΠ»ΡΠΈΠ½ΠΎΠΉ, Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ ΠΏΠΎΡ ΠΈ Π²ΠΎΠ»ΠΎΠΊΠΎΠ½, Π±ΠΈΠΎΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ. ΠΠ°ΡΠ΅Π»Π΅Π½ΠΈΠ΅ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΠΌΠ΅ΡΠ΅Π½ΡΠΌΠΈ ΠΌΡΠ»ΡΡΠΈΠΏΠΎΡΠ΅Π½ΡΠ½ΡΠΌΠΈ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΠΏΡΠΏΠΎΡΠ½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π°ΡΠΈΠΊΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΡΠ΅ΠΌΡ ΡΠΏΠΎΡΠΎΠ±Π°ΠΌΠΈ: ΡΡΠ°ΡΠΈΡΠ½ΡΠΌ, Π΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ°. ΠΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΈ ΡΠΎΠ»ΡΠΈΠ½Π΅ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ², ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈΠ·ΠΌΠ΅ΡΡΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΠ’Π’-ΡΠ΅ΡΡΠ°. Π‘ΡΠ°ΡΠΈΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ» ΠΏΠΎΠ»ΡΡΠΈΡΡ Π½ΠΎΡΠΈΡΠ΅Π»ΠΈ Ρ ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΡΠΌ ΠΏΠΎΠΊΡΡΡΠΈΠ΅ΠΌ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΊΠ»Π΅ΡΠΊΠΈ Π² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΌ ΡΠ°ΡΠΏΠΎΠ»Π°Π³Π°Π»ΠΈΡΡ Π² Π²Π΅ΡΡ
Π½Π΅ΠΉ ΡΡΠ΅ΡΠΈ ΠΌΠ°ΡΡΠΈΠΊΡΠ°. ΠΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΎΠΊΠ°Π·Π°Π»ΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π΅Π½ ΡΠΎΠ»ΡΠΊΠΎ Π΄Π»Ρ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΡΠΎΠ»ΡΠΈΠ½ΠΎΠΉ Π±ΠΎΠ»Π΅Π΅ 500 ΠΌΠΊΠΌ, Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎ ΠΎΡ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠ° ΠΏΠΎΡ. ΠΠ΅ΡΠΎΠ΄ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ° Π±ΡΠ» ΡΡΡΠ΅ΠΊΡΠΈΠ²Π΅Π½ ΡΠΎΠ»ΡΠΊΠΎ Π΄Π»Ρ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Ρ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ ΠΏΠΎΡ 20-30 ΠΌΠΊΠΌ, Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎ ΠΎΡ ΡΠΎΠ»ΡΠΈΠ½Ρ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°. ΠΠΈΠΎΡΠ΅Π·ΠΎΡΠ±ΠΈΡΡΠ΅ΠΌΡΠ΅ Π½Π΅ΡΠΊΠ°Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎ-Π»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π°, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ, ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΡΡΠΈΠΌΠΈ Π±ΠΈΠΎΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ Π΄Π»Ρ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΏΠ»Π°ΡΡΠΈΠΊΠΈ Π΄Π΅ΡΠ΅ΠΊΡΠΎΠ² ΡΡΠ΅Π½ΠΎΠΊ Π±ΡΡΡΠ½ΠΎΠΉ ΠΏΠΎΠ»ΠΎΡΡΠΈ, ΠΈΠΌΠ΅ΡΡ ΡΡ
ΠΎΠ΄Π½ΠΎΠ΅ Ρ ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠΌ Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΡΡΡΠΎΠ΅Π½ΠΈΠ΅. ΠΡΠ±ΠΎΡ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ Π΅Π³ΠΎ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΡΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ - ΡΠΎΠ»ΡΠΈΠ½Ρ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°, Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠ° ΠΏΠΎΡ ΠΈ Π²ΠΎΠ»ΠΎΠΊΠΎΠ½, ΠΊΠΎΡΠΎΡΡΠ΅, Π² ΡΠ²ΠΎΡ ΠΎΡΠ΅ΡΠ΅Π΄Ρ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΡΡ ΡΡΠ»ΠΎΠ²ΠΈΡΠΌΠΈ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°