Reaction Based Fluorescent Probes for Hydrogen Sulfide

A reaction based fluorescence turn-on strategy for hydrogen sulfide (H₂S) was developed. This strategy was based on a H₂S-specific Michael addition–cyclization sequence. Other biological thiols such as cysteine and glutathione did not pursue the reaction and therefore did not turn on the fluorescence/consume the substrates. The probes showed good selectivity and sensitivity for hydrogen sulfide

TSS and TA contents in Ponkan fruit during development and delayed harvest.

<p>The error bars represent the standard errors. LSDs represent least significant differences at the 0.05 level, and were calculated with data from stages S5 to S8.</p

Expressions of genes related to citrate synthesis in Ponkan fruit cultivated in a greenhouse and open field.

<p>The error bars represent the standard errors. LSDs represent least significant differences at the 0.05 level.</p

Primers used for quantitative real-time PCR analysis.

<p>Primers used for quantitative real-time PCR analysis.</p

Citrate contents of Ponkan fruit during low temperature and water stress treatments.

<p>CK: the control; LT: low temperature; WS: water stress; LT+WS: low temperature plus water stress. The error bars represent the standard errors. LSDs represent least significant differences at the 0.05 level.</p

Expressions of genes related to citrate degradation in Ponkan fruit cultivated in greenhouse and open field.

<p>The error bars represent the standard errors. LSDs represent least significant differences at the 0.05 level.</p

Real-time temperature during treatment of Ponkan plants.

<p>Real-time temperature during treatment of Ponkan plants.</p

Expressions of genes related to citrate synthesis in Ponkan fruit during low temperature and water stress treatments.

<p>The error bars represent the standard errors. LSDs represent least significant differences at the 0.05 level. CK: the control; LT: low temperature; WS: water stress; LT+WS: low temperature plus water stress.</p

Contents of organic acids in Ponkan fruit during development and delayed harvest.

<p>The error bars represent the standard errors. LSDs represent least significant differences at the 0.05 level, and were calculated with data from stages S5 to S8.</p