35 research outputs found
The pattern of late gadolinium enhancement by cardiac MRI in fulminant myocarditis and its prognostic implication: a two-year follow-up study
BackgroundMyocardial fibrosis, as quantified by late gadolinium enhancement (LGE) in cardiac magnetic resonance (CMR), provides valuable prognostic information for patients with myocarditis. However, due to the low incidence rate of fulminant myocarditis (FM) and accordingly small sample size, the knowledge about the role of LGE to patients with FM is limited.Methods and resultsA total of 44 adults with viral-FM receiving the Chinese treating regimen were included in this retrospective study. They were divided into the low LGE group and the high LGE group according to the ratio of LGE to left ventricular mass (LGE mass%). CMR exams and LGE were performed after hemodynamic assistance at discharge in all patients with FM. Routine echocardiography parameters and global longitudinal strain (GLS) at discharge and at 2-year follow-up were obtained and then compared. Both left ventricular ejection fraction (LVEF) and GLS showed no significant difference in both groups at discharge, whereas significant differences were observed at 2-year follow-up between two groups. Moreover, there were significant improvements of LVEF and GLS in the low LGE group, but not in the high LGE group during the 2-year period. Furthermore, LGE mass% was negatively correlated with GLS and LVEF.ConclusionsThere were two distinct forms of LGE presentation in patients with FM. Moreover, the cardiac function of patients with low LGE was significantly better than those with high LGE at 2-year follow-up. LGE mass% at discharge provided significant prognosis information about cardiac function of patients with FM
Downregulation of the CD151 protects the cardiac function by the crosstalk between the endothelial cells and cardiomyocytes via exosomes
An electron-hole rich dual-site nickel catalyst for efficient photocatalytic overall water splitting
Vast majority of photocatalysts for hydrogen production relies on additional sacrificial agents and noble metal cocatalysts. It is of great importance yet challenging to achieve photocatalytic overall water splitting with decent performance. Here, the authors report Ni2P based photocatalyst assisted by H2O2-craking reaction for overall water splitting with H2 and O2 production of 1507 μmol h−1 g−1 H2 and 702 μmol h−1 g−1
Application of Helicopter-Borne and Ground–Airborne Electromagnetic Detection to Tunnel Engineering Investigation
The Cedaya-S340 Holgutu Highway Construction Project is located in the Mongolian Autonomous Prefecture of Bayingolin in the Xinjiang Uygur Autonomous Region. It is an important traffic channel that connects Luntai County and Hejing County at the southern foot of Tianshan Mountains. As the major component of the highway project, the Huola Mountain Tunnel has a sharp topographic relief, and, therefore, commonly used land geophysical detection instruments cannot work on it. Therefore, we conducted a qualitative survey on the ground-to-air and airborne electromagnetic detection methods used at the Huola Mountain Tunnel site to provide basic data for the design of highway tunnels. The geophysical survey summarized the ground–airborne frequency-domain electromagnetic method (GAFEM) and the helicopter-borne time-domain electromagnetic method (HTEM) developed by Jilin University, and measured 15 measuring lines. Apparent resistivity imaging was performed for each section, and the results were consistent. This study comprehensively analyzed the apparent resistivity profile and geological mapping data. Then, the study inferred the major stratigraphic boundaries, fault fracture zones, rock fragmentation, weakness, karst development, and water content in accordance with background value, low-resistivity anomaly shape, low-resistivity anomaly value, and gradient value in the apparent resistivity profile. Finally, the study identified the scope of two main low-resistivity anomalies, located at the tunnel entrance and exit, respectively, which are basically consistent with the known fault location. The results of this study show that on the basis of the apparent resistivity maps of GAFEM and HTEM, the overall distribution law is basically consistent with site landform, hydrogeology, tectonic geology, and aerial image data. The results provide guidance for the construction of the Huola Mountain Tunnel and ensure the construction safety and progress of the tunnel
Metabolomic Analysis on the Mechanism of Nanoselenium Biofortification Improving the Siraitia grosvenorii Nutritional and Health Value
Nanoselenium (nano-Se) foliar application is crucial for enhancing plant health. However, the mechanism by which nano-Se biofortification promotes the nutritional components of Siraitia grosvenorii remains unclear. In this study, nano-Se foliar application increased the carbohydrate and amino acid contents, including glucose (23.6%), fructose (39.7%), sucrose (60.6%), tryptophan (104.5%), glycine (85.9%), tyrosine (78.4%), phenylalanine (60.1%), glutamic acid (63.4%), and proline (52.5%). Nano-Se application enhanced apigenin (3.8 times), syringic acid (0.7 times), and 4-hydroxy-3,5-dimethoxycinnamic acid (1.4 times) of the phenylpropane biosynthesis pathways. Importantly, the SgCDS (31.1%), CYP-P450 (39.1%), and UGT (24.6%) were induced by nano-Se, which enhanced the mogroside V content (16.2%). Compared to the control, nano-Se treatment dramatically enhanced aromatic substances, including 2-butanone (51.9%), methylpropanal (146.3%), n-nonanal dimer (141.7%), pentanal (52.5%), and 2-pentanone (46.0%). In summary, nano-Se improves S. grosvenorii quality by increasing nutrients and volatile organic compounds and adjusting the phenylpropane pathway
Rapid detection of H5 subtype avian influenza virus using CRISPR Cas13a based-lateral flow dipstick
Due to its high mortality rate, highly pathogenic avian influenza (HPAI), a notifiable animal illness designated by the World Organisation for Animal Health (WOAH), has caused enormous financial losses to the poultry sector. The H5 subtype of avian influenza virus (H5-AIV) is regarded as the most common highly pathogenic avian influenza virus (HPAIV) that threatens public health and safety. Virus isolation and reverse transcription quantitative PCR (RT-qPCR) are usually used to detect H5-AIV and are important for the timely diagnosis and control of H5-AIV. However, these methods are time-consuming and require a significant amount of effort. In this study, we established a recombinase-aided amplification (RAA) combined with CRISPR-Cas13a and lateral flow dipstick (LFD) assay for the detection of H5-AIV. The results showed that the process can be completed within 40 min at 37°C. The method had a detection limit of 0.1 copy/μL, which was comparable to the RT-qPCR. There was no cross-reactivity with H3-AIV, H7-AIV, H9-AIV, H10-AIV, IBV, NDV, RVA and DAstV. The kappa value of RT-RAA-Cas13a-LFD and RT-qPCR in 380 clinical samples was 0.89 (κ>0.75). In conclusion, we established a convenient, efficient and accurate method to detect H5-AIV, and the results can be visualized and interpreted using LFD, which can be adapted to the needs of grassroots laboratories and field-deployable assays. This approach provides a new perspective for clinical H5-AIV diagnosis and has great potential for application in clinical quarantine of the poultry farming
Establishment and application of quadruple fluorescence quantitative RT-PCR method for the identification of waterfowl astrovirus
Avian astrovirus can infect a variety of poultry species and cause viral diarrhea, with a wide epidemic range strong pathogenicity and a high incidence. Among them, Duck astrovirus 3(DAstV-3), Duck astrovirus 4(DAstV-4), Goose astrovirus 1(GoAstV-1) and Goose astrovirus 2(GoAstV-2) are four types of astroviruses newly discovered in waterfowl in recent years. In order to realize the rapid detection of these four kinds of waterfowl stellate viruses, specific primers and probes were engineered to target a highly conserved region of ORF1b gene of DAstV-3, GoAstV-1 and GoAstV-2 and the ORF2 gene of DAstV-4, and a quadruple fluorescence quantitative RT-PCR method was developed. The results indicate that the method established in this study has good specificity and no cross reactivity with other pathogens. This method can detect viruses with a minimum concentration of 1 × 101 copies/μL for DAstV-4, GoAstV-1 and GoAstV-2, respectively, while the minimum concentration for DAstV-3 is 1 × 102 copies/μL. Compared with the routinely used RT-PCR method, the limit of detection by the multiplex RT-PCR lower. Both intra- and inter-assay variability tests revealed excellent reproducibility. This method was then used to analyze 269 field samples, and the results were verified by genome sequencing. In conclusion, this study presents a sensitive, accurate, and specific method for detecting DAstV-3, DAstV-4, GoAstV-1, and GoAstV-2 in a single reaction, enabling the monitoring and differential diagnosis of these four types of waterfowl astroviruses
Nanoselenium Foliar Applications Enhance the Nutrient Quality of Pepper by Activating the Capsaicinoid Synthetic Pathway
Overexpression of CD151 promotes cardiac hypertrophy and fibrosis.
(a) Representative gross morphologies of the hearts in mice subjected to different treatments. And the ratios of heart weight to body weight in mice with diverse treatments. (b) Echocardiography analysis of LVEF% and FS%. (c) Hemodynamic parameters were measured by the Millar cardiac catheter system. (d) H&E staining represented the areas of CMs (left). Scale bars, 50 μm. The areas of CMs were analyzed by Image Pro Plus (right). (e)Representative images of Sirius Red staining of the heart sections from mice with different treatments (left) and the quantification analysis of cardiac fibrosis (right). Scale bars, 50 μm. Sham+ NS (n = 8), TAC + NS (n = 6), TAC + rAAV2-ICAM2-GFP (n = 6), TAC + rAAV2-ICAM2-CD151 (n = 6), TAC + rAAV2-ICAM2-shCD151 (n = 8). Data are expressed as mean ± SEM.</p
The expression of CD151 in heart tissue of mice.
BackgroundHeart failure (HF) is the last stage in the progression of various cardiovascular diseases. Although it is documented that CD151 contributes to regulate the myocardial infarction, the function of CD151 on HF and involved mechanisms are still unclear.Method and resultsIn the present study, we found that the recombinant adeno-associated virus (rAAV)-mediated endothelial cell-specific knockdown of CD151-transfected mice improved transverse aortic constriction (TAC)-induced cardiac function, attenuated myocardial hypertrophy and fibrosis, and increased coronary perfusion, whereas overexpression of the CD151 protein aggravated cardiac dysfunction and showed the opposite effects. In vitro, the cardiomyocytes hypertrophy induced by PE were significantly improved, while the proliferation and migration of cardiac fibroblasts (CFs) were significantly reduced, when co-cultured with the CD151-silenced endothelial cells (ECs). To further explore the mechanisms, the exosomes from the CD151-silenced ECs were taken by cardiomyocyte (CMs) and CFs, verified the intercellular communication. And the protective effects of CD151-silenced ECs were inhibited when exosome inhibitor (GW4869) was added. Additionally, a quantitative proteomics method was used to identify potential proteins in CD151-silenced EC exosomes. We found that the suppression of CD151 could regulate the PPAR signaling pathway via exosomes.ConclusionOur observations suggest that the downregulation of CD151 is an important positive regulator of cardiac function of heart failure, which can regulate exosome-stored proteins to play a role in the cellular interaction on the CMs and CFs. Modulating the exosome levels of ECs by reducing CD151 expression may offer novel therapeutic strategies and targets for HF treatment.</div