SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis-3

<p><b>Copyright information:</b></p><p>Taken from "SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis"</p><p>BMC Molecular Biology 2005;6():22-22.</p><p>Published online 1 Dec 2005</p><p>PMCID:PMC1314889.</p><p></p>tated plasmid DNA. g) α-Flag Western blot showing expression of mutant construct in 293T cells. h) Sequencing result of the mutation, mutated residues are highlighted in red

SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis-2

<p><b>Copyright information:</b></p><p>Taken from "SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis"</p><p>BMC Molecular Biology 2005;6():22-22.</p><p>Published online 1 Dec 2005</p><p>PMCID:PMC1314889.</p><p></p>d plasmid DNA. c) α-Flag Western blot showing expression of mutant construct in 293T cells. d) Sequencing result of the mutation, mutated residue is highlighted in red

SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis-1

<p><b>Copyright information:</b></p><p>Taken from "SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis"</p><p>BMC Molecular Biology 2005;6():22-22.</p><p>Published online 1 Dec 2005</p><p>PMCID:PMC1314889.</p><p></p> showing a novel BglII site discovered within the sequence

SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis-0

<p><b>Copyright information:</b></p><p>Taken from "SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis"</p><p>BMC Molecular Biology 2005;6():22-22.</p><p>Published online 1 Dec 2005</p><p>PMCID:PMC1314889.</p><p></p>cleotides and each successive window is shifted forward 4 nucleotides, ensuring minimal overlap. b) Example of all the possible sequences generated for each of the first two search windows using the moving window algorithm

Additional file 5: of Identification of genes regulating ovary differentiation after pollination in hazel by comparative transcriptome analysis

Table S5. DEGs in T-vs-FO paired comparisons. (XLS 3651 kb

Additional file 12: of Identification of genes regulating ovary differentiation after pollination in hazel by comparative transcriptome analysis

Table S12. FPKM values of DEGs encoding constituents of the floral quartet model (FQM). (XLSX 10 kb

Monitoring Phosphatidic Acid Formation in Intact Phosphatidylcholine Bilayers upon Phospholipase D Catalysis

We have monitored the production of the negatively charged lipid, 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphatidic acid acid (POPA), in supported lipid bilayers via the enzymatic hydrolysis of 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphocholine (PC), a zwitterionic lipid. Experiments were performed with phospholipase D (PLD) in a Ca²⁺ dependent fashion. The strategy for doing this involved using membrane-bound streptavidin as a biomarker for the charge on the membrane. The focusing position of streptavidin in electrophoretic-electroosmotic focusing (EEF) experiments was monitored via a fluorescent tag on this protein. The negative charge increased during these experiments due to the formation of POPA lipids. This caused the focusing position of streptavidin to migrate toward the negatively charged electrode. With the use of a calibration curve, the amount of POPA generated during this assay could be read out from the intact membrane, an objective that has been otherwise difficult to achieve because of the lack of unique chromophores on PA lipids. On the basis of these results, other enzymatic reactions involving the change in membrane charge could also be monitored in a similar way. This would include phosphorylation, dephosphorylation, lipid biosynthesis, and additional phospholipase reactions

Additional file 4: of Identification of genes regulating ovary differentiation after pollination in hazel by comparative transcriptome analysis

Table S4. DEGs in S-vs-T paired comparisons. (XLS 3850 kb

Additional file 7: of Identification of genes regulating ovary differentiation after pollination in hazel by comparative transcriptome analysis

Table S7. KEGG pathway enrichment analysis of DEGs identified in S-vs-T paired comparisons. (XLSX 22 kb

Additional file 3: of Identification of genes regulating ovary differentiation after pollination in hazel by comparative transcriptome analysis

Table S3. DEGs in F-vs-S paired comparisons. (XLSX 4773 kb