Tumor Catalytic–Photothermal Therapy with Yolk–Shell Gold@Carbon Nanozymes

Nanozymes, as a new generation of artificial enzymes, offer great opportunities in biomedical engineering and disease treatment. Synergizing the multiple intrinsic functions of nanozymes can improve their performance in biological systems. Here, we report a novel nanozyme with yolk–shell structure fabricated by combining a single gold nanoparticle core with a porous hollow carbon shell nanospheres (Au@HCNs). Au@HCNs exhibited enzyme-like activities similar to horseradish peroxidase and oxidase under an acidic environment, showing the ability of ROS generation. More importantly, the ROS production of Au@HCNs was significantly improved upon 808 nm light irradiation by the photothermal effect, which is often used for tumor therapy. Cellular and animal studies further demonstrated that the efficient tumor destruction was achieved through the combination of light-enhanced ROS and photothermal therapy. These results implied that the intrinsic enzyme-like activity and photothermal conversion of nanozymes can be synergized for efficient tumor treatment, providing a proof-of-concept of tumor catalytic–photothermal therapy based on nanozymes

Controllable Synthesis of Gold Nanorod/Conducting Polymer Core/Shell Hybrids Toward in Vitro and in Vivo near-Infrared Photothermal Therapy

Photothermal therapy (PTT) is a minimally invasive tumor treatment technology, and is regarded as a potential anticancer strategy because of its targeted destruction and low toxicity. Specifically, near-infrared light-induced PTT has attracted intriguing interest because of the high transparency of tissue, blood, and water. However, effective PTT generally requires the assistance of photothermal agents. Gold nanorods (GNRs) and conducting polymer are often used as photothermal materials because of their high absorption efficiency and photothermal conversion efficiency. Herein, we combined GNRs with poly­(<i>o</i>-methoxyaniline) (POMA, a polyaniline derivative) to form GNRs/POMA core/shell hybrids through the surfactant-assisted chemical oxidative polymerization route and studied their photothermal conversion properties. The configuration of GNRs/POMA core/shell hybrids has been precisely controlled through adjusting the monomer concentration, and the relationship between morphology and absorption band of GNRs/POMA core/shell hybrids has been revealed. Finally, the in vitro and in vivo experiments were performed, and the results indicated that the GNRs/POMA core/shell hybrids with optimized absorbance at around 808 nm exhibited the best performance on photothermal therapy under 808 nm NIR laser irradiation

El Diario de Pontevedra : periódico liberal: Ano XXVIII Número 8146 - 1911 xullo 8

Circular maps of the V. parahaemolyticus CHN25 chromosomes. (a) and (b) represent the larger and smaller chromosomes of V. parahaemolyticus CHN25, respectively. Each circle in the grey lines, except for the two innermost circles, illustrates specific features on the plus (outer region) and minus (inner region) strands. The lines and boxes in the three outermost circles are coloured according to the COG categories. The circles indicate the following from the outside inwards: first circle, predicted protein-coding genes; second circle, classified essential genes, including cell division, replication, transcription, translation, and amino acid metabolism; third circle, tRNA genes and rRNA operons; fourth circle, GC-skew (values above zero are red, values below zero are blue); fifth circle, GC content. (TIF 22070 kb

Additional file 2: Figure S2. of Genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (VpaCspA and VpaCspD) in Vibrio parahaemolyticus CHN25 during low-temperature survival

Circular maps of the V. parahaemolyticus CHN25 plasmids. (a)-(c): each circle in the grey lines, except for the two innermost circles, illustrates specific features on the plus (outer region) and minus (inner region) strands. Lines and boxes in the three outermost circles are coloured according to the COG categories. The circles indicate the following from the outside inwards: first circle, predicted protein-coding genes; second circle, GC-skew (values above zero in red, values below zero in blue); and third circle, GC content. (TIF 15096 kb