Expression of the gene for main intrinsic polypeptide (MIP): separate spatial distributions of MIP and beta-crystallin gene transcripts in rat lens development

The main intrinsic polypeptide (MIP) is the major protein present in the lens fiber cell membrane and is the product of a gene which, as far as is known, is expressed only in the lens. We have used in situ hybridization and immunofluorescence microscopy to characterize the expression of this gene during the course of development in the rat. At progressive stages of lens morphogenesis, we find that synthesis of the protein is closely tied to the accumulation of MIP mRNA in cells that are committed to terminal differentiation, first in the elongating presumptive primary lens fibers and later in the secondary fibers as they differentiate from the anterior epithelial cells. The transcripts accumulate in the basal cytoplasm of the primary fibers and in the cytoplasm which surrounds the cell nucleus in the secondary fibers. We have compared this pattern of expression with that of a gene for a cytoplasmic protein, beta-crystallin beta-A1/A3. In sharp contrast to the localized concentrations seen for the MIP mRNA, beta-A1/A3 transcripts are relatively uniformly distributed throughout the cytoplasm. Neither MIP nor crystallin gene appears to be transcriptionally active in the undifferentiated epithelial cell, but transcripts from the beta-A1/A3 gene appear earlier in fiber cell differentiation than do those from the gene for MIP

Method and apparatus for an optical function generator for seamless tiled displays

Producing seamless tiled images from multiple displays includes measuring a luminance profile of each of the displays, computing a desired luminance profile for each of the displays, and determining a spatial gradient profile of each of the displays based on the measured luminance profile and the computed desired luminance profile. The determined spatial gradient profile is applied to a spatial filter to be inserted into each of the displays to produce the seamless tiled display image

Specific volumes of the Zr41.2Ti13.8Cu12.5Ni10.0Be22.5 alloy in the liquid, glass, and crystalline states

The specific volumes of the Zr41.2Ti13.8CU12.5Ni10.0Be2.25 alloy as a function of temperature, T, are determined by employing an image digitizing technique and numerical calculation methods applied to the electrostatically levitated spherical alloy. The linear fitting of the volumes of the alloy in the liquid, V-l, glass, V-g, and crystalline V-c, states in the temperature ranges shown in parentheses are V-l(T) = 0.1583 + 8.877 x 10(-6)T(cm^(3)/g) (700-1300 K); V-g(T) = 0.1603 + 5.528 x 10^(-6)T (400-550 K); V-c(T) = 0.1583 + 6.211 x 10(-6)T(400-850 K). The average volume thermal expansion coefficients within the temperature ranges are determined to be 5.32, 3.39, and 3.83 x 10^(-5) (1/K) for the liquid, glass, and crystalline states, respectively

Method and apparatus for calibrating a tiled display

A display system that can be calibrated and re-calibrated with a minimal amount of manual intervention. To accomplish this, one or more cameras are provided to capture an image of the display screen. The resulting captured image is processed to identify any non-desirable characteristics, including visible artifacts such as seams, bands, rings, etc. Once the non-desirable characteristics are identified, an appropriate transformation function is determined. The transformation function is used to pre-warp the input video signal that is provided to the display such that the non-desirable characteristics are reduced or eliminated from the display. The transformation function preferably compensates for spatial non-uniformity, color non-uniformity, luminance non-uniformity, and other visible artifacts

Method and apparatus for calibrating a display using an array of cameras

The present invention overcomes many of the disadvantages of the prior art by providing a display that can be calibrated and re-calibrated with a minimal amount of manual intervention. To accomplish this, the present invention provides one or more cameras to capture an image that is projected on a display screen. In one embodiment, the one or more cameras are placed on the same side of the screen as the projectors. In another embodiment, an array of cameras is provided on either or both sides of the screen for capturing a number of adjacent and/or overlapping capture images of the screen. In either of these embodiments, the resulting capture images are processed to identify any non-desirable characteristics including any visible artifacts such as seams, bands, rings, etc. Once the non-desirable characteristics are identified, an appropriate transformation function is determined. The transformation function is used to pre-warp the input video signal to the display such that the non-desirable characteristics are reduced or eliminated from the display. The transformation function preferably compensates for spatial non-uniformity, color non-uniformity, luminance non-uniformity, and/or other visible artifacts