No significant differences of Th2 cells in the SI LP and serum total IgE, UDE-specific IgE and IgG1 levels between UDE- and PBS-administered mice.

<div><p>(A) Frequency and number of IL-4/GFP⁺ CD4⁺ T cells in the SI LP of UDE-versus PBS-administered control 4get mice.</p> <p>(B) Serum total IgE, UDE-specific IgE, and IgG1 secretion analyzed by ELISA.</p> <p>NS= not significant, OD₄₅₀=optical density at absorbance 450nm.</p></div

Significant increase of IL-4-expressing eosinophils in the SI LP of UDE-administered mice.

<div><p>(A) Cells isolated from SI LP were stained with CD11b, CD11c, and MHC class II and analyzed by flow cytometry. MHC class II ^lowCD11b^high CD11c^int cells (R1) are eosinophils. Among MHC class II^high cells, CD11b ^intCD11c^int cells (R4) and CD11c^high cells (R2 and R3) correspond to macrophages and dendritic cells, respectively.</p> <p>(B) Proportion and number of eosinophils, macrophages, and dendritic cells in the SP of UDE- versus PBS-administered control mice.</p> <p>(C) Antigen presenting cells isolated from SI LP were stained with CD40, CD80, and CD86 and analyzed by flow cytometry. Empty, solid line histograms represent cells from UDE-administered (red) versus PBS-administered control mice (black). Shaded histograms represent cells stained with isotype control of UDE-administered (dark gray) versus PBS-administered control mice (gray).</p> <p>(D) Mean fluorescence intensity of IL-4/GFP expressed by APCs from the SI LP of 4get mice were analyzed by flow cytometry.</p> <p>(E) Analysis of CD11b^highCD11c^int cells from LP of WT and ΔdblGATA mice for eosinophil markers CCR3 and Siglec F.</p> <p>*p<0.05, **p < 0.01, NS= not significant, LP: lamina propria, EO: eosionophil, DC: dendritic cell, Mϕ: macrophage, MFI: mean fluorescence intensity.</p></div

Decreased number of CD4⁺ T cells in the SI LP of UDE-administered mice.

<div><p>(A) Cells isolated from spleen (SP), mesenteric lymph node (MLN), and small intestinal lamina propria (SI LP) were stained with TCRβ and CD19 and analyzed by flow cytometry.</p> <p>(B) Frequency of T and B cells in the SP, MLN, and SI LP of UDE- versus PBS-administered control mice.</p> <p>(C) T Cells from SP, MLN, and SI LP were stained with CD4 and CD8α and analyzed by flow cytometry.</p> <p>(D) Frequency of CD4 and CD8 cells in the SP, MLN, and SI LP of UDE- versus PBS-administered control mice.</p> <p>(E) Total number of T/B cells and CD4/CD8 cells in the SP, MLN, and SI LP of UDE- versus PBS administered control mice.</p> <p>*p < 0.05, **p < 0.01, NS=not significant.</p></div