Associations between Aquaglyceroporin Gene Polymorphisms and Risk of Stroke among Patients with Hypertension

Background: Dysregulations ofAQP7andAQP9were found to be related to lipid metabolism abnormality, which had been provento be one of the mechanisms of stroke. However, limited epidemiological studies explore the associations betweenAQP7andAQP9and the risk of stroke among patients with hypertension in China. Aims: We aimed to investigate the associations between genetic variants in AQP7andAQP9and the risk of stroke among patients with hypertension, as well as to explore gene-gene andgene-environment interactions. Methods: Baseline blood samples were drawn from 211 cases with stroke and 633 matched controls. Genomic DNA was extracted by a commercially available kit. Genotyping of 5 single nucleotide polymorphisms (SNPs) in AQP7 (rs2989924, rs3758269, and rs2542743) and AQP9 (rs57139208, rs16939881) was performed by the polymerase chain reaction assay with TaqMan probes. Results: Participants with the rs2989924 GG genotype were found to be with a 1.74-fold increased risk of stroke compared to those with the AA+AG genotype, and this association remained significant after adjustment for potential confounders (odds ratio (OR): 1.74, 95% confidence interval (CI): 1.23-2.46). The SNP rs3758269 CC+TT genotype was found to be with a 33% decreased risk of stroke after multivariate adjustment (OR: 0.67, 95% CI: 0.45-0.99) compared to the rs3758269 CC genotype. The significantly increased risk of stroke was prominent among males, patients aged 60 or above, and participants who were overweight and with a harbored genetic variant in SNP rs2989924. After adjusting potential confounders, the SNP rs3758269 CT+TT genotype was found to be significantly associated with a decreased risk of stroke compared to the CC genotype among participants younger than 60 years old or overweight. No statistically significant associations were observed between genotypes of rs2542743, rs57139208, or rs16939881 with the risk of stroke. Neither interactions nor linkage disequilibrium had been observed in this study. Conclusions: This study suggests that SNPs rs2989924 and rs3758269 are associated with the risk of stroke among patients with hypertension, while there were no statistically significant associations between rs2542743, rs57139208, and rs16939881 and the risk of stroke being observed

Optimal Disturbance Control for Energy Feedback of Gas Water Heaters

In order to improve the recovery and utilization of hot water energy in gas water heater pipes, a self-powered solution is proposed in this study. The proposed solution focuses on improving energy collection efficiency by increasing the heat transfer coefficient of the water flow and tracking the maximum output power of the temperature plate. The design of heat transfer structure, power characteristics of temperature plate and circuit conversion are analyzed. In order to verify the feasibility of the proposed solution, a test device was constructed for physical testing and verification. The experimental results demonstrate the successful implementation of the self-powered gas water heater. The special structure and perturbation method used enable the experimental device to achieve the maximum power output, and the experimental device can continuously provide 300mW output power. This research contributes to the development of energy -efficient and self-powered gas water heater solutions, offering potential benefits for energy saving and sustainable use in the residential sector

General displacement arch-cantilever element method for stress analysis of arch dam

AbstractBased on the general displacement method and the basic hypothesis of the trial load method, a new advanced trial load method, the general displacement arch-cantilever element method, was proposed to derive the transformation relation of displacements and loads between the surface nodes and middle plane nodes. This method considers the nodes on upstream and downstream surfaces of the arch dam to be exit nodes (master nodes), and the middle plane nodes to be slave nodes. According to the derived displacement and load transformation matrices, the equilibrium equation treating the displacement of middle plane nodes as a basic unknown variable is transformed into one that treats the displacement of upstream and downstream nodes as a basic unknown variable. Because the surface nodes have only three degrees of freedom (DOF), this method can be directly coupled with the finite element method (FEM), which is used for foundation simulation to analyze the stress of the arch dam with consideration of dam-foundation interaction. Moreover, using the FEM, the nodal load of the arch dam can be easily obtained. Case studies of a typical cylindrical arch dam and the Wudongde arch dam demonstrate the robustness and feasibility of the proposed method

Nonlinear Floquet dynamics of spinor condensates in an optical cavity: Cavity-amplified parametric resonance

We investigate Floquet dynamics of a cavity-spinor Bose-Einstein condensate coupling system via periodic modulation of the cavity pump laser. Parametric resonances are predicted and we show that due to cavity feedback-induced nonlinearity the spin oscillation can be amplified to all orders of resonance, thus facilitating its detection. Real-time observation on Floquet dynamics via cavity output is also discussed

Crystal structure of tubulin folding cofactor A from Arabidopsis thaliana and its β-tubulin binding characterization

AbstractMicrotubules are composed of polymerized α/β-tubulin heterodimers. Biogenesis of assembly-competent tubulin dimers is a complex multistep process that requires sequential actions of distinct molecular chaperones and cofactors. Tubulin folding cofactor A (TFCA), which captures β-tubulin during the folding pathway, has been identified in many organisms. Here, we report the crystal structure of Arabidopsis thaliana TFC A (KIESEL, KIS), which forms a monomeric three-helix bundle. The functional binding analysis demonstrated that KIS interacts with β-tubulin in plant. Furthermore, mutagenesis studies indicated that the α-helical regions of KIS participate in β-tubulin binding. Unlike the budding yeast TFC A, the two loop regions of KIS are not required for this interaction suggesting a distinct binding mechanism of TFC A to β-tubulin in plants.Structured summaryMINT-7968902, MINT-7968915, MINT-7968951, MINT-7968966: KIS (uniprotkb:O04350) physically interacts (MI:0915) with Tub9 (uniprotkb:P29517) by anti tag coimmunoprecipitation (MI:0007)MINT-7968928: KIS (uniprotkb:O04350) and Tub9 (uniprotkb:P29517) physically interact (MI:0915) by bimolecular fluorescence complementation (MI:0809