DataSheet_1_Clinical efficacy of weight loss herbal intervention therapy and lifestyle modifications on obesity and its association with distinct gut microbiome: A randomized double-blind phase 2 study.docx

GoalsTo assess the efficacy and safety of Chinese Medicine Prescription “W-LHIT” in subjects with simple obesity, and to explore its potential mechanism of action.MethodsThirty-seven patients aged 18 to 60 from Wei-En hospital (Weifang City, Shandong, China), participated in a double blinded, placebo-controlled study. Subjects were randomly divided into 2 groups, 18 in treatment and 19 in placebo group. The treatment group took the “W-LHIT” capsules for two months, while the control group received placebo capsules. Both groups accepted healthy lifestyle education materials. After a 2-month treatment, the placebo group transferred to open-label treatment after unblinding.Results72.22% participants in the treatment group lost more than 5% of their body weight, compared with 36.84% in the placebo group (p ConclusionW-LHIT significantly improved body weight and comorbid conditions without obvious adverse reaction or rebound weight gain. These effects were associated with increased abundance of probiotics in gut microbiota. W-LHIT may have a potential for treating obesity in conjunction with healthy lifestyle modifications.</p

Additional file 3: Figure S3. of MicroRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway

Immunohistochemical analysis in tissue sections of GFP-labeled tumors isolated from mice injected with H460 and H1975 cells transfected with NC-LV, OE-miR-330-3p-LV, or anti-miR-330-3p-LV. Relative quantitative analysis of immunohistochemistry for PCNA (a), cyclinD1 (b), apoptosis-related proteins (c, d), and DNA methyltransferases (e–g). All data were presented as mean of three independent experiments ± SD. *P < 0.05, **P < 0.01, #P < 0.001, one-way ANOVA test. (TIF 941 kb

Additional file 2: Figure S2. of MicroRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway

miR-330-3p suppresses apoptosis of NSCLC cells. a Quantitative analysis of relative protein expression for Bcl-2 detected by western blotting. b Quantitative analysis of relative protein expression for Bax detected by western blotting in each group. c Quantitative analysis of relative protein expression for cleaved caspase3 detected by western blotting in each group. All data were presented as mean of three independent experiments ± SD. *P < 0.05, **P < 0.01, #P < 0.001, one-way ANOVA test. (TIF 523 kb

Additional file 4: Figure S4. of MicroRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway

miR-330-3p is relevant to DNMT1 and DNMT3A in NSCLC cells. a DNMT1 protein expression was quantified by western blotting analysis. b Quantitative analysis of DNMT3A detected by western blotting. c DNMT3B protein expression was assessed by western blotting. d Quantitative analysis of p16INK4A detected by western blotting. Error bars indicate mean ± SD for three independent experiments. *P < 0.05, **P < 0.01, #P < 0.001. P value was calculated by one-way ANOVA test. (TIF 625 kb