Feedback cooling of a cantilever's fundamental mode below 5 mK

We cool the fundamental mechanical mode of an ultrasoft silicon cantilever from a base temperature of 2.2 K to 2.9 +/- 0.3 mK using active optomechanical feedback. The lowest observed mode temperature is consistent with limits determined by the properties of the cantilever and by the measurement noise. For high feedback gain, the driven cantilever motion is found to suppress or "squash" the optical interferometer intensity noise below the shot noise level.Comment: 4 pages, 6 figure

Nuclear spin relaxation induced by a mechanical resonator

We report on measurements of the spin lifetime of nuclear spins strongly coupled to a micromechanical cantilever as used in magnetic resonance force microscopy. We find that the rotating-frame correlation time of the statistical nuclear polarization is set by the magneto-mechanical noise originating from the thermal motion of the cantilever. Evidence is based on the effect of three parameters: (1) the magnetic field gradient (the coupling strength), (2) the Rabi frequency of the spins (the transition energy), and (3) the temperature of the low-frequency mechanical modes. Experimental results are compared to relaxation rates calculated from the spectral density of the magneto-mechanical noise.Comment: 4 pages, 4 figure

Phase separation and vortex states in binary mixture of Bose-Einstein condensates in the trapping potentials with displaced centers

The system of two simultaneously trapped codensates consisting of $^{87}Rb$ atoms in two different hyperfine states is investigated theoretically in the case when the minima of the trapping potentials are displaced with respect to each other. It is shown that the small shift of the minima of the trapping potentials leads to the considerable displacement of the centers of mass of the condensates, in agreement with the experiment. It is also shown that the critical angular velocities of the vortex states of the system drastically depend on the shift and the relative number of particles in the condensates, and there is a possibility to exchange the vortex states between condensates by shifting the centers of the trapping potentials.Comment: 4 pages, 2 figure

Transcriptional profiling of the pea shoot apical meristem reveals processes underlying its function and maintenance

<p>Abstract</p> <p>Background</p> <p>Despite the importance of the shoot apical meristem (SAM) in plant development and organ formation, our understanding of the molecular mechanisms controlling its function is limited. Genomic tools have the potential to unravel the molecular mysteries of the SAM, and legume systems are increasingly being used in plant-development studies owing to their unique characteristics such as nitrogen fixation, secondary metabolism, and pod development. Garden pea (<it>Pisum sativum</it>) is a well-established classic model species for genetics studies that has been used since the Mendel era. In addition, the availability of a plethora of developmental mutants makes pea an ideal crop legume for genomics studies. This study aims to utilise genomics tools in isolating genes that play potential roles in the regulation of SAM activity.</p> <p>Results</p> <p>In order to identify genes that are differentially expressed in the SAM, we generated 2735 ESTs from three cDNA libraries derived from freshly micro-dissected SAMs from 10-day-old garden peas (<it>Pisum sativum </it>cv Torsdag). Custom-designed oligonucleotide arrays were used to compare the transcriptional profiles of pea SAMs and non-meristematic tissues. A total of 184 and 175 transcripts were significantly up- or down-regulated in the pea SAM, respectively. As expected, close to 61% of the transcripts down-regulated in the SAM were found in the public database, whereas sequences from the same source only comprised 12% of the genes that were expressed at higher levels in the SAM. This highlights the under-representation of transcripts from the meristematic tissues in the current public pea protein database, and demonstrates the utility of our SAM EST collection as an essential genetic resource for revealing further information on the regulation of this developmental process. In addition to unknowns, many of the up-regulated transcripts are known to encode products associated with cell division and proliferation, epigenetic regulation, auxin-mediated responses and microRNA regulation.</p> <p>Conclusion</p> <p>The presented data provide a picture of the transcriptional profile of the pea SAM, and reveal possible roles of differentially expressed transcripts in meristem function and maintenance.</p

Transcriptional profiling of the pea shoot apical meristem reveals processes underlying its function and maintenance

<p>Abstract</p> <p>Background</p> <p>Despite the importance of the shoot apical meristem (SAM) in plant development and organ formation, our understanding of the molecular mechanisms controlling its function is limited. Genomic tools have the potential to unravel the molecular mysteries of the SAM, and legume systems are increasingly being used in plant-development studies owing to their unique characteristics such as nitrogen fixation, secondary metabolism, and pod development. Garden pea (<it>Pisum sativum</it>) is a well-established classic model species for genetics studies that has been used since the Mendel era. In addition, the availability of a plethora of developmental mutants makes pea an ideal crop legume for genomics studies. This study aims to utilise genomics tools in isolating genes that play potential roles in the regulation of SAM activity.</p> <p>Results</p> <p>In order to identify genes that are differentially expressed in the SAM, we generated 2735 ESTs from three cDNA libraries derived from freshly micro-dissected SAMs from 10-day-old garden peas (<it>Pisum sativum </it>cv Torsdag). Custom-designed oligonucleotide arrays were used to compare the transcriptional profiles of pea SAMs and non-meristematic tissues. A total of 184 and 175 transcripts were significantly up- or down-regulated in the pea SAM, respectively. As expected, close to 61% of the transcripts down-regulated in the SAM were found in the public database, whereas sequences from the same source only comprised 12% of the genes that were expressed at higher levels in the SAM. This highlights the under-representation of transcripts from the meristematic tissues in the current public pea protein database, and demonstrates the utility of our SAM EST collection as an essential genetic resource for revealing further information on the regulation of this developmental process. In addition to unknowns, many of the up-regulated transcripts are known to encode products associated with cell division and proliferation, epigenetic regulation, auxin-mediated responses and microRNA regulation.</p> <p>Conclusion</p> <p>The presented data provide a picture of the transcriptional profile of the pea SAM, and reveal possible roles of differentially expressed transcripts in meristem function and maintenance.</p

Symmetric-Asymmetric transition in mixtures of Bose-Einstein condensates

We propose a new kind of quantum phase transition in phase separated mixtures of Bose-Einstein condensates. In this transition, the distribution of the two components changes from a symmetric to an asymmetric shape. We discuss the nature of the phase transition, the role of interface tension and the phase diagram. The symmetric to asymmetric transition is the simplest quantum phase transition that one can imagine. Careful study of this problem should provide us new insight into this burgeoning field of discovery.Comment: 6 pages, 3 eps figure

Enhanced heat capacity and a new temperature instability in superfluid He-4 in the presence of a constant heat flux near T-lambda

We present the first experimental evidence that the heat capacity of superfluid 4He, at temperatures very close to the lambda point Tλ, is enhanced by a constant heat flux Q. The heat capacity at constant Q, CQ, is predicted to diverge at a temperature Tc(Q)<Tλ at which superflow becomes unstable. In agreement with previous measurements, we find that dissipation enters our cell at a temperature, TDAS(Q), below the theoretical value, Tc(Q). We argue that TDAS(Q) can be accounted for by a temperature instability at the cell wall, and is therefore distinct from Tc(Q). The excess heat capacity we measure has the predicted scaling behavior as a function of T and Q, but it is much larger than predicted by current theory

Reorientation transition of ultrathin ferromagnetic films

We demonstrate that the reorientation transition from out-of-plane to in-plane magnetization with decreasing temperature as observed experimentally in Ni-films on Cu(001) can be explained on a microscopic basis. Using a combination of mean field theory and perturbation theory, we derive an analytic expression for the temperature dependent anisotropy. The reduced magnetization in the film surface at finite temperatures plays a crucial role for this transition as with increasing temperature the influence of the uniaxial anisotropies is reduced at the surface and is enhanced inside the film.Comment: 4 pages(RevTeX), 3 figures (EPS

Longitudinal imaging of microvascular remodelling in proliferative diabetic retinopathy using adaptive optics scanning light ophthalmoscopy

Purpose To characterise longitudinal changes in the retinal microvasculature of type 2 diabetes mellitus (T2DM) as exemplified in a patient with proliferative diabetic retinopathy (PDR) using an adaptive optics scanning light ophthalmoscope (AOSLO). Methods A 35-year-old T2DM patient with PDR treated with scatter pan-retinal photocoagulation at the inferior retina 1 day prior to initial AOSLO imaging along with a 24-year-old healthy control were imaged in this study. AOSLO vascular structural and perfusion maps were acquired at four visits over a 20-week period. Capillary diameter and microaneurysm area changes were measured on the AOSLO structural maps. Imaging repeatability was established using longitudinal imaging of microvasculature in the healthy control. Results Capillary occlusion and recanalisation, capillary dilatation, resolution of local retinal haemorrhage, capillary hairpin formation, capillary bend formation, microaneurysm formation, progression and regression were documented over time in a region 2° superior to the fovea in the PDR patient. An identical microvascular network with same capillary diameter was observed in the control subject over time. Conclusions High-resolution serial AOSLO imaging enables in vivo observation of vasculopathic changes seen in diabetes mellitus. The implications of this methodology are significant, providing the opportunity for studying the dynamics of the pathological process, as well as the possibility of identifying highly sensitive and non-invasive biomarkers of end organ damage and response to treatment