EEG Feature Analysis Related to Situation Awareness Assessment and Discrimination

In order to discriminate situation awareness (SA) levels on the basis of SA-sensitive electroencephalography (EEG) features, the high-SA (HSA) group and low-SA (LSA) groups, which are representative of two SA levels, were classified according to the situation awareness global assessment technology (SAGAT) scores measured in the multi-attribute task battery (MATB) II tasks. Furthermore, three types of EEG features, namely, absolute power, relative power, and slow-wave/fast-wave (SW/FW), were explored using spectral analysis. In addition, repeated analysis of variance (ANOVA) was conducted in three brain regions (frontal, central, and parietal) × three brain lateralities (left, middle, and right) × two SA groups (LSA and HSA) to explore SA-sensitive EEG features. The statistical results indicate a significant difference between the two SA groups according to SAGAT scores; moreover, no significant difference was found for the absolute power of four waves (delta (δ), theta (θ), alpha (α), and beta (β)). In addition, the LSA group had a significantly lower β relative power than the HSA group in central and partial regions. Lastly, compared with the HSA group, the LSA group had higher θ/β and (θ + α)/(α + β) in all analyzed brain regions, higher α/β in the parietal region, and higher (θ + α)/β in all analyzed regions except for the left and right laterality in the frontal region. The above SA-sensitive EEG features were fed into principal component analysis (PCA) and the Bayes method to discriminate different SA groups, and the accuracies were 83.3% for the original validation and 70.8% for the cross-validation. The results provide a basis for real-time assessment and discrimination of SA by investigating EEG features, thus contributing to monitoring SA decrement that might lead to threats to flight safety

EEG Feature Analysis Related to Situation Awareness Assessment and Discrimination

In order to discriminate situation awareness (SA) levels on the basis of SA-sensitive electroencephalography (EEG) features, the high-SA (HSA) group and low-SA (LSA) groups, which are representative of two SA levels, were classified according to the situation awareness global assessment technology (SAGAT) scores measured in the multi-attribute task battery (MATB) II tasks. Furthermore, three types of EEG features, namely, absolute power, relative power, and slow-wave/fast-wave (SW/FW), were explored using spectral analysis. In addition, repeated analysis of variance (ANOVA) was conducted in three brain regions (frontal, central, and parietal) × three brain lateralities (left, middle, and right) × two SA groups (LSA and HSA) to explore SA-sensitive EEG features. The statistical results indicate a significant difference between the two SA groups according to SAGAT scores; moreover, no significant difference was found for the absolute power of four waves (delta (δ), theta (θ), alpha (α), and beta (β)). In addition, the LSA group had a significantly lower β relative power than the HSA group in central and partial regions. Lastly, compared with the HSA group, the LSA group had higher θ/β and (θ + α)/(α + β) in all analyzed brain regions, higher α/β in the parietal region, and higher (θ + α)/β in all analyzed regions except for the left and right laterality in the frontal region. The above SA-sensitive EEG features were fed into principal component analysis (PCA) and the Bayes method to discriminate different SA groups, and the accuracies were 83.3% for the original validation and 70.8% for the cross-validation. The results provide a basis for real-time assessment and discrimination of SA by investigating EEG features, thus contributing to monitoring SA decrement that might lead to threats to flight safety

The immunomodulation of acetylcholinesterase in zhikong scallop Chlamys farreri.

BACKGROUND: Acetycholinesterase (AChE; EC 3.1.1.7) is an essential hydrolytic enzyme in the cholinergic nervous system, which plays an important role during immunomodulation in vertebrates. Though AChEs have been identified in most invertebrates, the knowledge about immunomodulation function of AChE is still quite meagre in invertebrates. METHODOLOGY: A scallop AChE gene was identified from Chlamys farreri (designed as CfAChE), and its open reading frame encoded a polypeptide of 522 amino acids. A signal peptide, an active site triad, the choline binding site and the peripheral anionic sites (PAS) were identified in CfAChE. The recombinant mature polypeptide of CfAChE (rCfAChE) was expressed in Pichia pastoris GS115, and its activity was 71.3±1.3 U mg(-1) to catalyze the hydrolysis of acetylthiocholine iodide. The mRNA transcripts of CfAChE were detected in haemocytes, hepatopancreas, adductor muscle, mantle, gill, kidney and gonad, with the highest expression level in hepatopancreas. The relative expression level of CfAChE mRNA in haemocytes was both up-regulated after LPS (0.5 mg mL(-1)) and human TNF-α (50 ng mL(-1)) stimulations, and it reached the highest level at 12 h (10.4-fold, P<0.05) and 1 h (3.2-fold, P<0.05), respectively. After Dichlorvos (DDVP) (50 mg L(-1)) stimulation, the CfAChE activity in the supernatant of haemolymph decreased significantly from 0.16 U mg(-1) at 0 h to 0.03 U mg(-1) at 3 h, while the expression level of lysozyme in the haemocytes was up-regulated and reached the highest level at 6 h, which was 3.0-fold (P<0.05) of that in the blank group. CONCLUSIONS: The results collectively indicated that CfAChE had the acetylcholine-hydrolyzing activity, which was in line with the potential roles of AChE in the neuroimmune system of vertebrates which may help to re-balance the immune system after immune response

The complete mitochondrial genome of the mudskipper, Boleophthalmus pectinirostris (Gobiiformes, Oxudercidae) from Beibu Bay

The mudskipper, Boleophthalmus pectinirostris (B. pectinirostris), is an amphibious fish that lives in the intertidal mudflats. It is a cultured economic fish with nutritional and pharmacological value. Here, we report the complete mitochondrial genome sequence of B. pectinirostris, which is 17,111 base pairs (55.3% A + T content) in length and consists of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and a 1453 bp D-loop region. The complete mitochondrial genome of B. pectinirostris will provide useful genetic information for future phylogenetic and taxonomic classification of B. pectinirostris

The complete mitochondrial genome of yellowfin seabream, Acanthopagrus latus (Percoiformes, Sparidae) from Beibu Bay

The yellowfin seabream, Acanthopagrus latus Houttuyn 1782, is a commercially and ecologically important species and a good model for studies of sexual differentiation. In this study, the complete mitochondrial genome of A. latus has been determined, which is 16,635 base pairs (54.3% A + T content) in length and consists of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and a 948 bp D-loop region. The phylogenetic analyses showed that A. latus has a close relationship with Acanthopagrus schlegelii Bleeker 1854

The complete mitochondrial genome of Scatophagus argus (Perciformes, Scatophagidae) from Beibu Bay and phylogenetic analysis

The spotted scat (Scatophagus argus, Linnaeus, 1766) is a subtropical fish that is widely distributed in the coastal waters of Indo-Pacific. Here, we report the complete mitochondrial genome of S. argus. The mitogenome is 16,783 base pairs (56.0% A + T content) in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a 1007 bp D-loop region. Phylogenetic analysis showed that the relationship between S. argus and Selenotoca multifasciata was close

The temporal expression of CfAChE mRNA in haemocytes after TNF-α stimulation for 1, 3, 6, 9 and 12 h.

<p>Data was expressed as the ratio of the CfAChE mRNA to the β-actin mRNA. The relative CfAChE expression level was determined for each group, and the unstimulated scallops (0 h) were used as the blank group, and scallops injected with PBS were used as the control group. Values were shown as mean ± S.E. (N = 6), and bars with * are significantly different (<i>P</i><0.05).</p

Tissue distribution of the CfAChE transcripts detected by SYBR Green RT-PCR.

<p>CfAChE transcript level in adductor muscle, mantle, gill, hepatopancreas, kidney and gonad of six adult scallops was normalized to that of haemocytes. Vertical bars represented the mean ± S.E. (N = 6), and bars with * are significantly different (<i>P</i><0.05).</p