22 research outputs found
Amphiphile-Mediated Ultrasmall Aggregation Induced Emission Dots for Ultrasensitive Fluorescence Biosensing
The development of
ultrasensitive and highly selective fluorescence
biosensors for diverse analytes is highly desirable but remains a
challenge. It is attributable to the scarcity of fluorogens with promising
brightness, stability, and nontoxicity, which primarily determine
the performance of fluorescence biosensors. Herein, we report the
design and preparation of aggregation induced emission (AIE) dots
with high brightness, exceptional colloidal stability, ultrasmall
size, and functional groups for developing ultrasensitive biosensor
through the electrostatic conjugation to biological molecules, and
use blemycin (BLM) as the proof-of-concept analyte. The recognition
and the subsequent cleavage of the quencher-labeled DNA (Q-DNA) by
BLM result in the formation of three-mer quencher-linked oligonucleotide
fragments (Q-DNA-1), which significantly decreases the amount of quenchers
anchored on AIE dot surfaces and subsequently reduces the fluorescence
resonance energy transfer (FRET) effect. As compared to the case in
which BLM is absent, remarkable fluorescence enhancement is observed,
and is dependent on BLM concentration. Thus, ultrasensitive fluorescence
detection of target BLM is realized, with a detection limit down to
3.4 fM, the lowest value reported so far. Moreover, the proposed fluorescence
biosensor has also been successfully utilized for detection of BLM
spiked in human serum samples. The as-proposed strategy not only significantly
improves the selectivity and sensitivity of BLM assay, but also allows
the ultrasensitive detection of a variety of bioactive molecules by
simply changing the specific target recognition substances, thus providing
a versatile fluorescence platform, and showing great potential to
be applied in chemo-/bioanalysis and clinical biomedicine
Clinical Improvements in Lower Back and Leg Pain Syndrome of Adult Idiopathic Scoliotic Patients.
<p>SD indicates standard deviation; ODI, Oswestry disability index; VAS, visual analogue scale.</p
41-year-old female with a BMI of 27.7 diagnosed with thoracolumbar idiopathic scoliosis.
<p>(A) anterior-posterior standing and (B) standing lateral full-length spinal preoperative X-rays showing coronal imbalance and sagittal thoracolumbar kyphosis; (C) anterior-posterior standing and (D) standing lateral full-length spinal X-rays: the first post-surgical erect X-rays showing the correction and fusion; (E) anterior-posterior standing and (F) standing lateral full-length spinal X-rays: the two-year follow-up X-rays showed a good balance.</p
Procedure-related Complications of Adult Idiopathic Scoliotic Patients.
<p>Procedure-related Complications of Adult Idiopathic Scoliotic Patients.</p
Pre-existing Comorbidities of Adult Idiopathic Scoliotic Patients.
<p>Pre-existing Comorbidities of Adult Idiopathic Scoliotic Patients.</p
Summary of Demographics and Baseline Characteristics of Adult Idiopathic Scoliotic Patients.
<p>SD indicates standard deviation; BMI, body mass index.</p
Preoperative Radiographic Measures of Adult Idiopathic Scoliotic Patients.
<p>SD indicates standard deviation; CSVL, center sacral vertical line.</p
Final Follow-up Radiographic Measures of Adult Idiopathic Scoliotic Patients.
<p>SD indicates standard deviation; CSVL, center sacral vertical line.</p
Green Carbon Dots Illuminate Biogenic Nanohybrids toward Soft, Piezo/Photoactive, and Physically Transient Nanogenerators
Green electronics comprising degradable materials are
ideal solutions
for electronic wastes. Here, we report the construction of a soft,
piezo/photoactive, and physically transient biogenic nanogenerator.
A green luminescent, biogenic hybrid, carbon quantum dot (CQD)/cellulose
nanocrystal (CNC)/polyhydroxybutyrate (PHB) nanofiber is manufactured
with electrospinning technology. The synergy of CQD and CNC enables
the enhancement of degradability of PHB. Simultaneously, the piezoelectric
CNC makes a contribution to the piezoelectric response of the hybrid
nanofiber. The appropriate inclusion of the CQD allows the creation
of luminous, local stress concentration points that further enhance
the piezoelectric response of the hybrid nanofiber. The output voltage
and output current of the nanofibrous CQD/CNQ/PHB-based piezoelectric
nanogenerator are 6.7 and 4.4 times higher than those of the state-of-the-art
pristine PHB-based equivalent, respectively. This type of nanogenerator
is also self-adaptive to changes in external mechanical stimuli. Moreover,
the soft nanogenerator as a tactile sensor exhibits high reliability
in the real-time dynamic sensing of athletic motions. Importantly,
with a remarkable combination of piezoelectricity and photoluminescence,
the biogenic nanogenerator array demonstrates the first application
as a bimodal piezo/photo encryption alarm system. It shows great potential
in the smart home and security field. We suppose that the multiple-responsive
biogenic nanohybrids are very promising in green transient optoelectronics
Postoperative Radiographic Measures of Adult Idiopathic Scoliotic Patients.
<p>SD indicates standard deviation; CSVL, center sacral vertical line.</p