IDs for the ID converter

The columns respectively represent gene symbol, transcript symbol, ensembl gene id and ensembl transcript id

R code for the differential expression analyses

This ".R" format file contains the R code for differential expression analyse

Rab27a expression showed mesenchymal, G3 subtype, and IDH1 wild-type preferences.

<p>For each patient, the TCGA and CGGA subtypes are annotated as previously reported and are listed in the upper part together with the IDH1 mutation status, which was obtained from the CGGA database. Correlated genes are supervised by clustering according to Rab27a expression, from low to high. The positively and negatively correlated genes are shown in the lower panel (marked pink and blue on the right, respectively). NA, not available.</p

Rab27a protein expression was correlated with glioma grade progression and prognosis.

<p>Immunohistochemical staining showed that Rab27a expression increased with grade in gliomas. The sections were counterstained with hematoxylin and mounted with Permount. The immunochemistry scoring system was as follows (A): normal brain tissue: 0 (a), astrocytoma: 1 (WHO Grade II) (b), anaplastic astrocytoma: 4 (WHO Grade III) (c), and glioblastoma: 9 (WHO Grade IV) (d). (B) Histogram showed staining score increased from normal to WHO Grade IV. (C) By kaplan meier analysis including 96 GBMs, we found high Rab27b protein expression harbored significant short overall survival.</p

The prognostic value of Rab27a in the CGGA and validation datasets.

<p>Except for WHO Grade IV patients in the GSE16011 dataset (<b>I,</b> p = 0.28), patients from every grade could be divided into two groups with significantly different prognoses according to Rab27a expression level: <b>A</b> (p = 0.167), <b>B</b> (p = 0.160), <b>C</b> (p = 0.045) – anaplastic gliomas and GBMs in CGGA data; <b>D</b> (p<0.01)<b>, </b><b>E</b> (p<0.01), <b>F</b> (p<0.01) – astrocytoma, anaplastic gliomas, and GBMs in Rembrandt data; <b>G</b> (p = 0.02), GBMs in TCGA data; and <b>H</b> (p<0.01), anaplastic gliomas in GSE16011 data). (<b>High</b>, higher Rab27a expression than the median. <b>Low</b>, lower Rab27a expression than the median).</p

Cox Hazard Regression Analyses of Clinicopathologic Factors and the RAB27a for Overall Survival in the Independent Cohort (GBMs, n = 96).

<p>Cox Hazard Regression Analyses of Clinicopathologic Factors and the RAB27a for Overall Survival in the Independent Cohort (GBMs, n = 96).</p

Gene sets enriched in WHO Grade IV glioma samples with high Rab27A expression.

<p>Gene sets enriched in WHO Grade IV glioma samples with high Rab27A expression.</p

Gene set variation analysis with Rab27a expression.

<p>Gene set variation analysis with Rab27a expression was analyzed using the GSVA package. Gene expression signatures of migration were generated from the GO list. Rab27a expression is listed from left to right, starting with the highest expression level. A high enrichment score indicates a positive correlation with Rab27a expression, and a low enrichment score indicates the reverse.</p

Clinical characteristics of 220 glioma patients.

<p>Karnofsky Performance Scale (KPS).</p

The difference in Rab27a expression in normal brain and glioma tissues in CGGA and 2 other validation datasets.

<p>We compared Rab27a expression in normal, WHO Grade II, WHO Grade III, and WHO Grade IV gliomas using the CGGA dataset (A), GSE16011 (B), and Rembrandt (C). The P values were analyzed to assess differences between the groups. A single spot represents the Rab27a expression value of an individual patient, and the lines in the middle represent the mean expression values.</p