A Computational Approach to Estimating Nondisjunction Frequency in Saccharomyces cerevisiae.

Errors segregating homologous chromosomes during meiosis result in aneuploid gametes and are the largest contributing factor to birth defects and spontaneous abortions in humans. Saccharomyces cerevisiae has long served as a model organism for studying the gene network supporting normal chromosome segregation. Measuring homolog nondisjunction frequencies is laborious, and involves dissecting thousands of tetrads to detect missegregation of individually marked chromosomes. Here we describe a computational method (TetFit) to estimate the relative contributions of meiosis I nondisjunction and random-spore death to spore inviability in wild type and mutant strains. These values are based on finding the best-fit distribution of 4, 3, 2, 1, and 0 viable-spore tetrads to an observed distribution. Using TetFit, we found that meiosis I nondisjunction is an intrinsic component of spore inviability in wild-type strains. We show proof-of-principle that the calculated average meiosis I nondisjunction frequency determined by TetFit closely matches empirically determined values in mutant strains. Using these published data sets, TetFit uncovered two classes of mutants: Class A mutants skew toward increased nondisjunction death, and include those with known defects in establishing pairing, recombination, and/or synapsis of homologous chromosomes. Class B mutants skew toward random spore death, and include those with defects in sister-chromatid cohesion and centromere function. Epistasis analysis using TetFit is facilitated by the low numbers of tetrads (as few as 200) required to compare the contributions to spore death in different mutant backgrounds. TetFit analysis does not require any special strain construction, and can be applied to previously observed tetrad distributions

Meson Form-factors and Wave-functions with Wilson fermions

Results for semi-leptonic form-factors for processes like $D \to K l \nu$ and the Bethe-Salpeter amplitudes (BSA) for pion and rho mesons are presented. The form-factor data is consistent with previous calculations. We find that the long distance fall-off of BSA for both $\pi$ and $\rho$ is very well fit by an exponential, but surprisingly the effective mass governing this fall-off is lighter than the pion's. Lastly, by studying the dependence of $\rho$ polarization on separation direction we show that there is a measureable $l=2$ state in addition to $l=0$ in the BSA for the rho. (Talk presented by R. Gupta at LATTICE92. Latex needs macro package espcrc2.sty)Comment: 4 pages including 4 PS figure

Midwest China Oral History and Archives Collection

Father\u27s life: parents decision to join Lutheran Church of China; role in and development of Sinyang Lutheran Church; involvement in community and social programs; experience with Red Spears in Sinyang; anti-Christian sentiment and imprisonment during 1927; as president of Lutheran Church of China, 1932 - 1938; views on denominationalism and ecumenism; activities in Chungking during Japanese war, plans for post-war church. Personal experience: education, thoughts about Christianity\u27s meaning for China as a students; reposnse to mission schools; comparison of being a student at Yale-in-China and Lutheran Theological Seminary in Shekow; recommendations for Lutheran Church.https://digitalcommons.luthersem.edu/china_histories/1019/thumbnail.jp

Evolution in range expansions with competition at rough boundaries.

When a biological population expands into new territory, genetic drift develops an enormous influence on evolution at the propagating front. In such range expansion processes, fluctuations in allele frequencies occur through stochastic spatial wandering of both genetic lineages and the boundaries between genetically segregated sectors. Laboratory experiments on microbial range expansions have shown that this stochastic wandering, transverse to the front, is superdiffusive due to the front's growing roughness, implying much faster loss of genetic diversity than predicted by simple flat front diffusive models. We study the evolutionary consequences of this superdiffusive wandering using two complementary numerical models of range expansions: the stepping stone model, and a new interpretation of the model of directed paths in random media, in the context of a roughening population front. Through these approaches we compute statistics for the times since common ancestry for pairs of individuals with a given spatial separation at the front, and we explore how environmental heterogeneities can locally suppress these superdiffusive fluctuations

A Critical Examination of Hypernova Remnant Candidates in M101. II. NGC 5471B

NGC 5471B has been suggested to contain a hypernova remnant because of its extraordinarily bright X-ray emission. To assess its true nature, we have obtained high-resolution images in continuum bands and nebular lines with the Hubble Space Telescope, and high-dispersion long-slit spectra with the Kitt Peak National Observatory 4-m echelle spectrograph. The images reveal three supernova remnant (SNR) candidates in the giant HII region NGC 5471, with the brightest one being the 77x60 pc shell in NGC 5471B. The Ha velocity profile of NGC 5471B can be decomposed into a narrow component (FWHM = 41 km/s) from the background HII region and a broad component (FWHM = 148 km/s) from the SNR shell. Using the brightness ratio of the broad to narrow components and the Ha flux measured from the WFPC2 Ha image, we derive an Ha luminosity of (1.4+-0.1)x10^39 ergs/s for the SNR shell. The [SII]6716,6731 doublet ratio of the broad velocity component is used to derive an electron density of ~700 cm^-3 in the SNR shell. The mass of the SNR shell is thus 4600+-500 Mo. With a \~330 km/s expansion velocity implied by the extreme velocity extent of the broad component, the kinetic energy of the SNR shell is determined to be 5x10^51 ergs. This requires an explosion energy greater than 10^52 ergs, which can be provided by one hypernova or multiple supernovae. Comparing to SNRs in nearby active star formation regions, the SNR shell in NGC 5471B appears truly unique and energetic. We conclude that the optical observations support the existence of a hypernova remnant in NGC 5471B.Comment: 27 pages, 9 figures, to appear in May 2002 issue of The Astronomical Journa

A Deep Chandra Observation of the Giant HII Region N11 I. X-ray Sources in the Field

A very sensitive X-ray investigation of the giant HII region N11 in the LMC was performed using the Chandra X-ray Observatory. The 300ks observation reveals X-ray sources with luminosities down to 10^32 erg/s, increasing by more than a factor of 5 the number of known point sources in the field. Amongst these detections are 13 massive stars (3 compact groups of massive stars, 9 O-stars and one early B-star) with log(Lx/Lbol)~-6.5 to -7, which may suggest that they are highly magnetic or colliding wind systems. On the other hand, the stacked signal for regions corresponding to undetected O-stars yields log(Lx/Lbol)~-7.3, i.e., an emission level comparable to similar Galactic stars despite the lower metallicity. Other point sources coincide with 11 foreground stars, 6 late-B/A stars in N11, and many background objects. This observation also uncovers the extent and detailed spatial properties of the soft, diffuse emission regions but the presence of some hotter plasma in their spectra suggests contamination by the unresolved stellar population.Comment: file including online material, accepted for publication by ApJ

Declining Burden of Plasmodium vivax in a Population in Northwestern Thailand from 1995 to 2016 before Comprehensive Primaquine Prescription for Radical Cure.

All Plasmodium cases have declined over the last decade in northwestern Thailand along the Myanmar border. During this time, Plasmodium vivax has replaced Plasmodium falciparum as the dominant species. The decline in P. falciparum has been shadowed by a coincidental but delayed decline in P. vivax cases. This may be due to early detection and artemisinin-based therapy, species-specific diagnostics, and bed net usage all of which reduce malaria transmission but not P. vivax relapse. In the absence of widespread primaquine use for radical cure against P. vivax hypnozoites, the decline in P. vivax may be explained by decreased hypnozoite activation of P. vivax relapses triggered by P. falciparum. The observed trends in this region suggest a beneficial effect of decreased P. falciparum transmission on P. vivax incidence, but elimination of P. vivax in a timely manner likely requires radical cure