THE INTRODUCED SABELLID POLYCHAETE TEREBRASABELLA HETEROUNCINATA IN CALIFORNIA: TRANSMISSION, METHODS OF CONTROL AND SURVEY FOR PRESENCE IN NATIVE GASTROPOD POPULATIONS

The sabellid polychaete Terebrasabella heterouncinata (Fitzhugh & Rouse 1999) has a unique life history in which larvae settle on the edge of gastropod shells and rely on shell deposition to create a tube with an opening to the exterior. This worm was accidentally imported to California, USA on abalone from South Africa in the 1980s and spread with abalone shipments to most culture facilities and some public aquaria throughout the state. Its ability to infest California's native gastropods has sparked concern regarding potential establishment in intertidal habitats adjacent to facilities that held sabellid-positive abalone. We examined the ability of T. heterouncinata to transmit between individual turban snails, Tegula funebralis. We found that transmission between T.funebralis did occur, but at a significantly slower rate than that between red abalone Haliotis rufescens. During 2002 to 2006 native gastropods (turban snails and limpets) were collected at most sabellid-exposed sites and no T. heterouncinata were detected; it thus appears that this species has not become established in California. Freshwater exposure was examined as a method to kill T. heterouncinala in shell fragments that may remain after abalone are removed from production or display units. Freshwater immersion for up to 8 hours but not 16 or 32 h resulted in survival of adults and/or larvae resident in brood chambers. In a similar study, motile T. heterouncinata larvae were found to survive up to 32 see of freshwater exposure, whereas none survived a 64-sec exposure. These data can be used by abalone culture and display facilities to establish reliable sanitization procedures to prevent T. heterouncinata transmission or reinfestation

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

The introduced sabellid polychaete Terebrasabella heterouncinata in California: Transmission, methods of control and survey for presence in native gastropod populations

The sabellid polychaete Terebrasabella heterouncinata (Fitzhugh & Rouse 1999) has a unique life history in which larvae settle on the edge of gastropod shells and rely on shell deposition to create a tube with an opening to the exterior. This worm was accidentally imported to California, USA on abalone from South Africa in the 1980s and spread with abalone shipments to most culture facilities and some public aquaria throughout the state. Its ability to infest California's native gastropods has sparked concern regarding potential establishment in intertidal habitats adjacent to facilities that held sabellid-positive abalone. We examined the ability of T. heterouncinata to transmit between individual turban snails, Tegula funebralis. We found that transmission between T.funebralis did occur, but at a significantly slower rate than that between red abalone Haliotis rufescens. During 2002 to 2006 native gastropods (turban snails and limpets) were collected at most sabellid-exposed sites and no T. heterouncinata were detected; it thus appears that this species has not become established in California. Freshwater exposure was examined as a method to kill T. heterouncinala in shell fragments that may remain after abalone are removed from production or display units. Freshwater immersion for up to 8 hours but not 16 or 32 h resulted in survival of adults and/or larvae resident in brood chambers. In a similar study, motile T. heterouncinata larvae were found to survive up to 32 see of freshwater exposure, whereas none survived a 64-sec exposure. These data can be used by abalone culture and display facilities to establish reliable sanitization procedures to prevent T. heterouncinata transmission or reinfestation

Green abalone, Haliotis fulgens infected with the agent of withering syndrome do not express disease signs under a temperature regime permissive for red abalone, Haliotis rufescens

All California abalone species have been shown to be susceptible to infection with the bacterial agent of abalone withering syndrome (WS), although expression of signs of the disease may vary between species and with environmental conditions. We examined thermal modulation of WS expression in green abalone Haliotis fulgens at temperatures mimicking El Niño (18.0°C) and La Niña (14.2°C) events in southern California. In contrast to results obtained from previous experiments with red abalone, H. rufescens, the higher temperature did not result in higher infection intensities of the causative agent of the disease nor increase in clinical signs of disease. These results demonstrate clear differences in thermal regulation of disease expression between abalone species, and provide further data suggesting that green abalone should be a target species of recovery efforts in southern California, where WS is endemic