4 research outputs found
Impact of propofol on neurotrophins.
<p>Densitometric quantifications of mRNA levels of BDNF and NT-3 in cortex and thalamus of P6 rats, analysed by qRT-PCR. Values represent mean normalised ratios of the densities of BDNF and NT-3 bands compared to the density of the control group (nβ=β6β7/point+SE). There was an effect of propofol treatment with a decrease of BDNF levels over time, which was significant after 6 hrs in the cortex [F(1,30)β=β66.5, p<0.001]. There was also a decrease in NT-3 levels, which was significant in the cortex after 6 hrs [F(1,28)β=β12.7, pβ=β0.004] and after 12 hrs in the thalamus [F(1,24)β=β3.5, pβ=β0.06].</p
Impact of propofol on survival promoting proteins.
<p>Densitometric quantifications of pAKT and pERK1/2 in the cortex and thalamus of P6 rats, analysed by Western blotting. Values represent mean normalised ratios of the densities of pAKT and pERK1/2 bands compared to the density of the control group (nβ=β6/point+SE). There was an effect of propofol treatment in decrease of pAKT levels over time in the thalamus, which was significant after 12 hrs [F(1,28)β=β5.6, pβ=β0.06]. Post-hoc analysis showed most pronounced decrease after 12 hrs (2-sample t-test). In the cortex there was a significant decrease of pERK1/2 levels over the time, which was significant after 6, 12 and 24 hrs [F(1,29)β=β12.7, pβ=β0.013].</p
Impact of propofol on key proteins involved in apoptotic signalling.
<p>Densitometric quantifications of caspase-3 and AIF in cortex and thalamus of P6 rats as analysed by Western blotting. Values represent mean normalised ratios of the densities of caspase-3 and AIF bands compared to densities of the control group (nβ=β5β6/point+SE). There was an effect of propofol treatment on caspase-3 levels over time, which was significant after 24 hrs in the cortex [F(1,29)β=β3.63, pβ=β0.06] and after 12 hrs in the thalamus [F(1,28)β=β3.1, pβ=β0.09).</p
Novel object recognition on P30 and P120: At the age of 30 days, both propofol treated animals (t(7)β=β7.45, ***qβ=β4.3Γ10<sup>β4</sup>) and controls (t(10)β=β6.30, ***qβ=β3.6Γ10<sup>β4</sup>) spent significantly more time with the novel object indicating their ability to discriminate the novel from the old object.
<p>Propofol (t(7)β=ββ1.44, qβ=β0.192) as well as control animals (t(10)β=ββ1.92, qβ=β0.168) failed to do so after a 24 hrs inter-trial interval. At P120 both groups spent a random amount of time with either of the objects after 6 hrs and also after a 24 hrs interval, indicating that they were unable to remember the old object. (n<sub>controls</sub>β=β12 animals, n<sub>propofol</sub>β=β8 animals).</p