List of Major and Sub Clades of SLC22 Family Members in Human and Mouse.

<p>List of Major and Sub Clades of SLC22 Family Members in Human and Mouse.</p

Unrooted Phylogeny of the SLC22 Transporter Family Using 163 Sequences of Various Organisms for Each of the 31 SLC22 Members.

<p>The sequences were aligned and the tree was generated using Clustal X 2.1 (using default parameters). The tree was viewed using Interactive Tree of Life (iTOL), an online tree viewing tool [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140569#pone.0140569.ref055" target="_blank">55</a>]. Accession numbers are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140569#pone.0140569.s007" target="_blank">S1 Table</a>. Bootstrap values are shown at each node of this phylogeny.</p

Overlay of Common and Subclade-specific Motifs on Human SLC22A6 2D Topology.

<p>(A) SLC22 family-conserved motifs versus subclade-conserved motifs. Overlaid on the 2D topology are the 16 evolutionarily-conserved family motifs (green/dark green) and the 11 subclade-specific motifs (red/blue). Conserved family motifs are assigned letters A-P in the order of appearance in human OAT1 (human SLC22A6). Subclade-specific motifs are either whole or partial (red/blue) and are assigned the numbers 1–11. (B) This table describes the order, region, and percent present of the motifs which appear in the inputted set, MEME#, E-value, and amino acid sequence of each of the subclade-specific motifs identified for the hOAT1 sequence (red). (C) This table describes the SLC22 family-conserved motifs that have been overlaid onto the hOAT1 sequence (green). It lists the motif letter and percent present in all 111 sequences used in the MEME analysis, the MEME#, E-value, and the amino acid sequence of hOAT1 associated with the particular motif letter. Additional sequence information can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140569#pone.0140569.s009" target="_blank">S3</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140569#pone.0140569.s010" target="_blank">S4</a> Tables.</p

Shared Motifs of SLC22 Family Transporters by MEME Analysis on 16 of the Sequences Used in the Analysis.

<p>Abbreviations of the SLC22 member number is on the far left (e.g., A15 = SLC22A15) and known common names are on the far right. In green are the SLC22 family-conserved motifs which are lettered (A-P) in the order that they appear in the SLC22A6 amino acid sequence. The majority of the sequences follow this motif order. In red are the subclade-specific motifs overlaid on the same 1D representation of each SLC22 sequence. The first box labeled the Large Extracellular Loop represents the predicted Large Extracellular Loop [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140569#pone.0140569.ref056" target="_blank">56</a>] and the second box labeled the Large Intracellular Loop represents the predicted Large Intracellular Loop. Note that each 1D representation is proportionally scaled such that each sequence length can be estimated in the scale near the bottom of the figure.</p