第949回千葉医学会例会・第19回千葉大学第三内科懇話会

<p>Accuracy of chlorogenic acid, rutin and isoquercitrin.</p

HPLC chromatogram of standardized extract of <i>M</i>. <i>nigra</i> leaves (2 mg/mL).

<p>(A) Batch 1. (B) Batch 2. (C) Batch 3. (D) Batch 4. (E) Batch 5.</p

Robustness of chlorogenic acid, rutin and isoquercitrin.

<p>Robustness of chlorogenic acid, rutin and isoquercitrin.</p

Calibration curves for chlorogenic acid, rutin and isoquercitrin.

<p>Calibration curves for chlorogenic acid, rutin and isoquercitrin.</p

IC₅₀ values of five standardized extracts of <i>M</i>. <i>nigra</i> leaves compared to kojic acid.

<p>The significant differences related to the IC₅₀ values on tyrosinase inhibition activity (*<i>p</i><0.05) were calculated by Student's t test.</p

Linearity of chlorogenic acid, rutin and isoquercitrin.

<p>Linearity of chlorogenic acid, rutin and isoquercitrin.</p

Quantification of chlorogenic acid, rutin and isoquercitrin of standardized extract of <i>M</i>. <i>nigra</i> leaves.

<p>The significant differences related to the concentration values of five batches in three experiments were calculated by one-way ANOVA with Tukey test (^<i>a</i><i>p</i><0.05 <i>vs</i> Batch 2. ^b<i>p</i><0.05 <i>vs</i> Batch 3. ^<i>c</i><i>p</i><0.05 <i>vs</i> Batch 1. ^<i>d</i><i>p</i><0.05 <i>vs</i> Batch 2. ^<i>e</i><i>p</i><0.05 <i>vs</i> Batch 3).</p

Specificity of the proposed method.

<p>(A) Acid hydrolysis and (B) Basic hydrolysis of diluted standardized extract of <i>M</i>. <i>nigra</i> leaves (2 mg/mL) λ = 354nm.</p

Tyrosinase inhibition activity profile of five standardized extracts of <i>M</i>. <i>nigra</i> leaves and kojic acid.

<p>Tyrosinase inhibition activity profile of five standardized extracts of <i>M</i>. <i>nigra</i> leaves and kojic acid.</p

Flow variation on robustness.

<p>(A) 0.3 mL/min (B) 0.5 mL/min (C) 0.7 mL/min.</p