Cyclic uniaxial tensile test of the different materials.

<p>(a) Strain-stress plots of the 1^st, 2^nd and 5^th cycle. (b) Plastic deformation (%) at the 2^nd cycle. (c) Young’s modulus of the 1^st and 2^nd cycle (n-3±SEM), *p<0.05, **p<0.01.</p

Values for Mechanical properties before and after 7 days of dynamic loading.

<p>Young’s Modulus (<b>top</b>) and ultimate tensile strength (<b>bottom</b>) calculated from stress curves before and after 7 days of uniaxial distension (n-2±SEM), *p<0.05, **p<0.01, ***p<0.001. Dotted lines represent values of healthy paravaginal tissues [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0149971#pone.0149971.ref024" target="_blank">24</a>].</p

Stress vs strain plots of the 4 different materials.

<p>Ramp uniaxial tensile test before (A) and after (B) 7 days under dynamic distention in an EBERS bioreactor.</p

Collagen production of cells cultured on scaffolds.

<p>This was measured by Sirius red assay. Absorbance measured at 490nm per gram of scaffold, n = 3±SEM ***p<0.001.</p

Summary of scaffold properties.

<p>Summary of scaffold properties.</p

Proliferation of cells on scaffolds assessed by assessing cell metabolic activity with AlamarBlue assay (Absorbance measured at 570nm).

<p>(n = 3 ±SEM). *p<0.05 **p<0.01.</p

Fluorescence microscopy and scaffold fibre second harmonic generation using confocal microscopy.

<p>Cells (<b>red</b>) and fibre SHG signal (<b>green</b>) for each of the 5 constructs imaged from the scaffold surface (0μm) to 20μm depth. All scale bars equivalent to 50μm.</p

Scanning electron microscopy images of scaffolds before cell seeding and after 2 weeks of culture with ADSC.

<p>Cells stained with DAPI and Sirius red.</p

Apparatus for co-electrospinning.

<p>Apparatus for co-electrospinning.</p