Cell type-specific regulation of choline acetyltransferase gene expression - Role of the neuron-restrictive silencer element and cholinergic-specific enhancer

This study demonstrates the presence of positive and negative regulatory elements within a 2336-base pair-long region of the rat choline acetyltransferase (ChAT) gene promoter that cooperate to direct cell type-specific expression in cholinergic cells. A 21-base pair-long neuron-restrictive silencer element (NRSE) was identified in the proximal part of this region. This element was recognized by the neuron-restrictive silencer factor (NRSF), previously shown to regulate expression of other neuron-specific genes. The ChAT NRSE was inactive in both cholinergic and non-cholinergic neuronal cells, but repressed expression from a heterologous promoter in non-neuronal cells. Specific deletion of this element allowed ChAT gene promoter activity in non-neuronal cells, and overexpression of NRSF repressed ChAT gene promoter activity in cholinergic cells. The distal part of the ChAT gene promoter showed cholinergic-specific enhancing activity, which stimulated promoter activity in cholinergic cells, but was inactive in non-cholinergic neuronal and non-neuronal cells. This enhancer region suppressed the activity of the ChAT NRSE in cholinergic cells, even after NRSF overexpression. Thus, at least two kinds of regulatory elements cooperate to direct ChAT gene expression to cholinergic neurons, namely a neuron-restrictive silencer element and a cholinergic-specific enhancer

CH 502 Church History II

1) To identify the key historical period of the Reformation and post- Reformation era. 2) To understand the major Reformation traditions—Lutheran, Anabaptist, Reformed, and Anglican—and their theologians, especially in terms of faithfulness to the teachings of Scripture, the nature of the church, the sacraments, and soteriology. 3) To grasp the significant issues pertaining to salvation in the Protestant Reformation and understand the issues relating to Roman Catholicism. 4) To understand the Roman Catholic response to the Protestant Reformation including the Council of Trent. 5) To recognize and evaluate the major issues involved in the Church’s ministries within the Christian community and its efforts to relate to the social and political structures of the modern world. 6) To explore the eighteenth-century Enlightenment’s impact on the church. 7) To articulate the Puritan, Pietist, and Methodist traditions, and to develop an increased awareness of the significant contributions of Wesleyanism to the broader Church. 8) To demonstrate an understanding of the First and Second Great Awakenings. 9) To understand the cultural and theological context of historical criticism and its effect on the nature and authority of Scripture. 10) To reflect on the problems and possibilities of church/state relations during this period. 11) To explore the interaction between church and culture, as the leading theologians developed their theologies. 12) To survey the major religious non-Christian traditions, with a view to the students’ future ministries in evangelism, nurture, and leadership. 13) To trace the rise of modern missions and how Christianity became a global religion.https://place.asburyseminary.edu/syllabi/2478/thumbnail.jp

To “Elevate Unfortunate Humanity” On-screen: Linking World War I and Protestant Missions through Silent Film

The purpose of this essay is to examine how post-World War I American Methodists used silent film to link the progress of the U.S. military with the advances of denominational missionaries overseas. Both the American army and the largest Protestant denomination in the U.S. were on separate yet intricately intertwined missions – one to promote the extension of American “democracy” and “civilization” by rescuing foreigners from the clutches of menacing dictators, the other to promote a version of U.S. Protestantism by saving foreigners from the grasp of non-Christian religions and religious practices

MICROSTRUCTURAL SURFACE INCORPORATION OF PHASE CHANGE MATERIALS FOR THERMAL MANAGEMENT APPLICATIONS

Thermal management strategies employed to mitigate the detrimental effects of elevated temperatures include the use of heat sinks or spreaders, thermal interface materials, and phase change materials (PCM), among others. PCM are substances capable of absorbing or releasing thermal energy when they undergo a phase transition. The goal of this thesis was to engineer a surface layer that contained a phase change material on top of a metallic component used for thermal dissipation and test its performance. The ideal conditions to create a porous anodic layer that could contain phase change material in an aluminum substrate were determined. Vacuum impregnation was used to incorporate the phase change material within the annealed anodic layer. Sealing materials were tested regarding their effectiveness to contain the PCM, their thermal conductivity and ability to withstand thermal cycles. Thermal tests were run to compare the behavior of samples containing PCM with those that were only anodized and raw aluminum. The use of the alkane eicosane as PCM, introduced in pores created by anodization techniques in an aluminum heat sink that served as substrate, resulted in specimens that presented lower temperatures during the heating cycles than those without PCM, proving the potential of this strategy to manage transient thermal loads.ESTEP, Monterey, CA 93943Outstanding ThesisLieutenant, United States NavyApproved for public release. Distribution is unlimited

CH 502 Church History II

Bettenson, Henry and Chris Maunder, eds. Documents of the Christian Church. New York: Oxford University Press, 1999. Gonzalez, Justo. The Story of Christianity. Volume 2. San Francisco: Harper and Row Publishers, 1985. Kerr, Hugh T., ed. Readings in Christian Thought. Nashville: Abingdon Press, 1990.https://place.asburyseminary.edu/syllabi/2958/thumbnail.jp