2 research outputs found
Reductive Dissolution of Tl(I)āJarosite by <i>Shewanella putrefaciens</i>: Providing New Insights into Tl Biogeochemistry
Thallium (Tl) is emerging as a metal of concern in countries
such
as China due to its release during the natural weathering of Tl-bearing
ore deposits and mining activities. Despite the high toxicity of Tl,
few studies have examined the reductive dissolution of Tl mineral
phases by microbial populations. In this study we examined the dissolution
of synthetic TlĀ(I)ājarosite, (H<sub>3</sub>O)<sub>0.29</sub>Tl<sub>0.71</sub>Fe<sub>2.74</sub>(SO<sub>4</sub>)<sub>2</sub>(OH)<sub>5.22</sub>(H<sub>2</sub>O)<sub>0.78</sub>, by <i>Shewanella
putrefaciens</i> CN32 using batch experiments under anaerobic
circumneutral conditions. FeĀ(II) concentrations were measured over
time and showed FeĀ(II) production (4.6 mM) in inoculated samples by
893 h not seen in mineral and dead cell controls. Release of aqueous
Tl was enhanced in inoculated samples whereby maximum concentrations
in inoculated and cell-free samples reached 3.2 and 2.1 mM, respectively,
by termination of the experiment. Complementary batch Tl/<i>S. putrefaciens</i> sorption experiments were conducted under
experimentally relevant pH (5 and 6.3) at a Tl concentration of 35
Ī¼M and did not show significant Tl accumulation by either live
or dead cells. Therefore, in contrast to many metals such as Pb and
Cd, <i>S. putrefaciens</i> does not represent a sink for
Tl in the environment and Tl is readily released from Tlājarosite
during both abiotic and biotic dissolution
Simultaneous Release of Fe and As during the Reductive Dissolution of PbāAs Jarosite by <i>Shewanella putrefaciens</i> CN32
Jarosites
are produced during metallurgical processing, on oxidized
sulfide deposits, and in acid mine drainage environments. Despite
the environmental relevance of jarosites, few studies have examined
their biogeochemical stability. This study demonstrates the simultaneous
reduction of structural FeĀ(III) and aqueous AsĀ(V) during the dissolution
of synthetic PbāAs jarosite (PbFe<sub>3</sub>(SO<sub>4</sub>,AsO<sub>4</sub>)<sub>2</sub>(OH)<sub>6</sub>) by <i>Shewanella
putrefaciens</i> using batch experiments under anaerobic circumneutral
conditions. FeĀ(III) reduction occurred immediately in inoculated samples
while AsĀ(V) reduction was observed after 72 h. XANES spectra showed
AsĀ(III) (14.7%) in the solid phase at 168 h coincident with decreased
aqueous AsĀ(V). At 336 h, XANES spectra and aqueous speciation analysis
demonstrated 20.2% and 3.0% of total As was present as AsĀ(III) in
the solid and aqueous phase, respectively. In contrast, 12.4% of total
Fe was present as aqueous FeĀ(II) and was below the detection limits
of XANES in the solid phase. TEM-EDS analysis at 336 h showed secondary
precipitates enriched in Fe and O with minor amounts of As and Pb.
Based on experimental data and thermodynamic modeling, we suggest
that structural FeĀ(III) reduction was thermodynamically driven while
aqueous AsĀ(V) reduction was triggered by detoxification induced to
offset the high AsĀ(V) (328 Ī¼M) concentrations released during
dissolution