Heatmap showing comparative expression of <i>Culicoides sonorensis</i> vitellogenesis target genes in response to blood feeding.

<p>Early blood is 2, 6, 12(pooled) and late blood is 36 h post ingestion, with Log₁₀ FPKM values indicated in legend. Further description of these genes can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098123#pone-0098123-t001" target="_blank">Table 1</a>, and detailed statistic and values of fold change can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098123#pone-0098123-t002" target="_blank">Table 2</a>.</p

Volcano plots of pairwise comparisons illustrating diet effects on differential gene expression in <i>C. sonorensis</i>.

<p>The volcano plot is composed of comparisons of replicate normalized transcriptional states between feeding conditions including teneral, early (2, 6, 12 h pooled) or late (36 h) blood or sucrose feeding. Log2 fold change for each unigene (dots) is shown; red dots indicate statistical significance (P≤0.01) of the pairwise comparison of net transcript abundance (up or down to right or left of midline, respectively).</p

Genetic components of vitellogenesis in <i>C. sonorensis</i>.

<p>Genetic components of vitellogenesis in <i>C. sonorensis</i>.</p

GO classification of the adult female midge unigene set.

<p>Classification and functional distrubuion of the 19,041 unigenes of the <i>C. sonorensis</i> transcriptome according to the 3 major classifications of the Gene Ontology: Biological Process, Molecular Function, and Cellular Component.</p

<i>Culicoides sonorensis</i> unigene assembly and filtering.

<p>Blue bars indicate the number of unigene sequences versus size that were present at the initial Trinity build. Red bars indicate the number and lengths of unigenes after ORF filtering, and green bars represent the final unigene build after clustering and manual inspection.</p

Transcriptome-level differential expression of vitellogenesis genes in blood-fed female C. sonorensis.

<p>Ten (teneral), BE (early blood-fed), BL (late blood-fed) transcriptomes.</p

Transcriptional response to blood feeding in female <i>Culicoides sonorensis</i>.

<p>Numbers of genes classified within GO-slim categories that were upregulated (blue) or downregulated (red) in response to blood feeding are shown, including comparisons of (A) teneral versus an early blood meal (B) teneral versus an late blood meal, and (C) early blood meal versus late blood meal. Note difference in vertical scale between A, B and C.</p

Transcriptome profiles and temporal expression analysis (early or late) across diet (sucrose or blood) in female <i>Culicoides sonorensis</i>.

<p>Comparison of the genes that were unique and shared between: A. teneral and early blood (EB) and sucrose (ES) meals (both 2, 6, 12 h post ingestion, pooled), or B. teneral and late blood (LB) and sucrose (LS) meals (both 36 h). Right (boxes): The number of genes with differential expression profiles (P≤0.01), and the percent of shared unigene (in parentheses).</p

Global response to blood and sucrose meals in female <i>Culicoides sonorensis</i>.

<p>Global transcript upregulation (blue) and downregulation (red) in response to a blood meal (A; early and late transcriptomes combined) or sucrose meal (B; early and late transcriptomes combined). Note difference in vertical scale between A and B.</p

Unigene BLASTx alignment to the nr protein database, and <i>Aedes</i> or <i>Culex</i> gene set.

<p>The number of translated <i>Culicoides sonorensis</i> unigene alignments that have matches to the databases are shown. Unique numbers show how many <i>Culicoides</i> matches were unique to each of the databases, and the unmapped genes had not homology matches in any of the databases.</p