Editorial: Advances in label free tissue imaging with laser scanning microscopy techniques

Advances in Label Free Tissue Imaging with Laser Scanning Microscopy Technique

Low loss photonic nanocavity via dark magnetic dipole resonant mode near metal

The dielectric-semiconductor-dielectric-metal 4 layered structure is a well-established configuration to support TM hybrid plasmonic modes, which have demonstrated significant advantages over pure photonic modes in structures without metal to achieve low loss resonant cavities at sub-diffraction limited volumes. The photonic modes with TE characteristics supported by the same 4 layered structure, on the other hand, are less studied. Here we show that a low loss photonic mode with TE01 characteristics exists in the dielectric-semiconductor-dielectric-metal 4 layered structure if a truncated cylindrical disk is chosen as the semiconductor core. This mode exhibits the lowest cavity loss among all resonant modes, including both pure photonic and hybrid plasmonic modes, at cavity radius <150 nm and within the wavelength range 620 nm to 685 nm, with a footprint ~0.83 (λ/2neff)2, physical size ~0.47 (λ/2neff)3 and a mode volume down to 0.3 (λ/2neff)3. The low cavity loss of this TE01 mode is attributed to its substantially reduced radiation loss to the far field by the creation of image charges through the metal response. Because of the low mode penetration in the metal, this photonic mode show equally low cavity loss near industry relevant metals such as Cu. Our study demonstrates an alternative to hybrid plamonic modes and metallo-dielectric modes to achieve low loss cavities with extremely small footprints

Rheological issues in carbon-based inks for additive manufacturing

As the industry and commercial market move towards the optimization of printing and additive manufacturing, it becomes important to understand how to obtain the most from the materials while maintaining the ability to print complex geometries effectively. Combining such a manufacturing method with advanced carbon materials, such as Graphene, Carbon Nanotubes, and Carbon fibers, with their mechanical and conductive properties, delivers a cutting-edge combination of low-cost conductive products. Through the process of printing the effectiveness of these properties decreases. Thorough optimization is required to determine the idealized ink functional and flow properties to ensure maximum printability and functionalities offered by carbon nanoforms. The optimization of these properties then is limited by the printability. By determining the physical properties of printability and flow properties of the inks, calculated compromises can be made for the ink design. In this review we have discussed the connection between the rheology of carbon-based inks and the methodologies for maintaining the maximum pristine carbon material properties

Dark field and coherent anti-stokes raman (DF-CARS) imaging of cell uptake of core-shell, magnetic-plasmonic nanoparticles

Magnetic-plasmonic, Fe3O4 -Au, core-shell nanoparticles are popular in many applications, most notably in therapeutics and diagnostics, and thus, the imaging of these nanostructures in biological samples is of high importance. These nanostructures are typically imaged in biological material by dark field scatter imaging, which requires an even distribution of nanostructures in the sample and, therefore, high nanoparticle doses, potentially leading to toxicology issues. Herein, we explore the nonlinear optical properties of magnetic nanoparticles coated with various thicknesses of gold using the open aperture z-scan technique to determine the nonlinear optical properties and moreover, predict the efficacy of the nanostructures in nonlinear imaging. We find that the magnetic nanoparticles coated with gold nano seeds and thinner gold shells (ca. 4 nm) show the largest nonlinear absorption coefficient β and imaginary part of the third-order susceptibility Im χ(3), suggesting that these nanostructures would be suitable contrast agents. Next, we combine laser dark field microscopy and epi-detected coherent anti-Stokes Raman (CARS) microscopy to image the uptake of magnetic-plasmonic nanoparticles in human pancreatic cancer cells. We show the epi-detected CARS technique is suitable for imaging of the magnetic-plasmonic nanoparticles without requiring a dense distribution of nanoparticles. This technique achieves superior nanoparticle contrasting over both epi-detected backscatter imaging and transmission dark field imaging, while also attaining label-free chemical contrasting of the cell. Lastly, we show the high biocompatibility of the Fe3O4 nanoparticles with ca. 4-nm thick Au shell at concentrations of 10–100 µg

The effects of a varied gold shell thickness on iron oxide nanoparticle cores in magnetic manipulation, T1 and T2 MRI contrasting, and magnetic hyperthermia

Fe3O4–Au core–shell magnetic-plasmonic nanoparticles are expected to combine both magnetic and light responsivity into a single nanosystem, facilitating combined optical and magnetic-based nanotheranostic (therapeutic and diagnostic) applications, for example, photothermal therapy in conjunction with magnetic resonance imaging (MRI) imaging. To date, the effects of a plasmonic gold shell on an iron oxide nanoparticle core in magnetic-based applications remains largely unexplored. For this study, we quantified the efficacy of magnetic iron oxide cores with various gold shell thicknesses in a number of popular magnetic-based nanotheranostic applications; these included magnetic sorting and targeting (quantifying magnetic manipulability and magnetophoresis), MRI contrasting (quantifying benchtop nuclear magnetic resonance (NMR)-based T1 and T2 relaxivity), and magnetic hyperthermia therapy (quantifying alternating magnetic-field heating). We observed a general decrease in magnetic response and efficacy with an increase of the gold shell thickness, and herein we discuss possible reasons for this reduction. The magnetophoresis speed of iron oxide nanoparticles coated with the thickest gold shell tested here (ca. 42 nm) was only ca. 1% of the non-coated bare magnetic nanoparticle, demonstrating reduced magnetic manipulability. The T1 relaxivity, r1, of the thick gold-shelled magnetic particle was ca. 22% of the purely magnetic counterpart, whereas the T2 relaxivity, r2, was 42%, indicating a reduced MRI contrasting. Lastly, the magnetic hyperthermia heating efficiency (intrinsic loss power parameter) was reduced to ca. 14% for the thickest gold shell. For all applications, the efficiency decayed exponentially with increased gold shell thickness; therefore, if the primary application of the nanostructure is magnetic-based, this work suggests that it is preferable to use a thinner gold shell or higher levels of stimuli to compensate for losses associated with the addition of the gold shell. Moreover, as thinner gold shells have better magnetic properties, have previously demonstrated superior optical properties, and are more economical than thick gold shells, it can be said that “less is more”

A framework for far-field infrared absorption microscopy beyond the diffraction limit

A framework is proposed for infrared (IR) absorption microscopy in the far-field with a spatial resolution below the diffraction limit. The sub-diffraction resolution is achieved by pumping a transient contrast in the population of a selected vibrational mode with IR pulses that exhibit alternating central minima and maxima, and by probing the corresponding absorbance at the same wavelength with adequately delayed Gaussian pulses. Simulations have been carried out on the basis of empirical parameters emulating patterned thin films of octadecyltrichlorosilane and a resolution of 250 nm was found when probing the CH2 stretches at 3.5 mu m with pump energies less than ten times the vibrational saturation threshold. (C) 2012 Optical Society of Americ

Ultrathin oxide controlled photocurrent generation through a metal–insulator– semiconductor heterojunction

Recent advances in nanoscale lasers, amplifiers, and nonlinear optical converters have demonstrated the unprecedented potential of metal–insulator–semiconductor (MIS) structures as a versatile platform to realize integrated photonics at the nanoscale. While the electric field enhancement and confinement have been discussed intensively in MIS based plasmonic structures, little is known about the carrier redistribution across the heterojunction and photocurrent transport through the oxide. Herein, we investigate the photo-generated charge transport through a single CdSe microbelt-Al2O3-Ag heterojunction with oxide thickness varying from 3 nm to 5 nm. Combining photocurrent measurements with finite element simulations on electron (hole) redistribution across the heterojunction, we are able to explain the loss compensation observed in hybrid plasmonic waveguides at substantially reduced pump intensity based on MIS geometry compared to its photonic counterpart. We also demonstrate that the MIS configuration offers a low-dark-current photodetection scheme, which can be further exploited for photodetection applications.</p

Thermal effects of mobile phones on human auricle region

Mobile phones have become an indispensable utility to modern society, with international use increasing dramatically each year. The GSM signal operates at 900 MHz, 1800 MHz and 2250 MHz, may potentially cause harm to human tissue. Yet there is no in silico model to aid design these devices to protect from causing potential thermal effect. Here we present a model of sources of heating in a mobile phone device with experimental verification during the phone call. We have developed this mobile phone thermal model using first principles on COMSOL® Multiphysics modelling platform to simulate heating effect in human auricle region due to mobile phone use. In particular, our model considered both radiative and non-radiative heating from components such as the lithium ion battery, CPU circuitry and the antenna. The model showed the distribution and effect of the heating effect due to mobile phone use and considered impact of battery discharge rate, battery capacity, battery cathode material, biological tissue distance, antenna radio-wave frequency and intensity. Furthermore, the lithium ion battery heating was validated during experiments using temperature sensors with an excellent agreement between simulated and experimental data (<1% variation). Mobile phone heating during a typical call has also been simulated and compared with experimental infrared thermographic imaging. Importantly, we found that 1800 MHz frequency of data transmission showed the highest temperature increase in the fat/water phantom used in this simulation. We also successfully compared heating distribution in human auricle region during mobile phone use with clinical thermographic images with reasonable qualitative and quantitative agreements. In summary, our model provides a foundation to conceive thermal and other physical effects caused by mobile phone use and allow for the understanding of potential negative health effects thus supporting and promoting personalized and preventive medicine using thermography

Characterisation and manipulation of polarisation response in plasmonic and magneto-plasmonic nanostructures and metamaterials

Optical properties of metal nanostructures, governed by the so-called localised surface plasmon resonance(LSPR) effects,have envoked intensive investigations in recent times owing to their fundamental nature and potential applications. LSPR scattering from metal nanostructures is expected to show the symmetry of the oscillation mode and the particle shape. Therefore, information on the polarisation properties of the LSPR scattering is crucial for identifying different oscillation modes within one particle and to distinguish differently shaped particles within one sample. On the contrary, the polarisation state of light itself can be arbitrarily manipulated by the inverse designed sample, known as metamaterials. Apart from polarisation state, external stimulus, e.g., magnetic field also controls the LSPR scattering from plasmonic nanostructures, giving rise to a new field of magneto-plasmonics. In this review, we pay special attention to polarisation and its effect in three contrasting aspects. First, tailoring between LSPR scattering and symmetry of plasmonic nanostructures, secondly, manipulating polarisation state through metamaterials and lastly, polarisation modulation in magneto-plasmonics. Finally, we will review recent progress in applications of plasmonic and magneto plasmonicnano structures and metamaterials in various fields

A practical approach for standardization of converse piezoelectric constants obtained from piezoresponse force microscopy

The ability to reliably measure electromechanical properties is crucial to the advancement of materials design for applications in fields ranging from biology and medicine to energy storage and electronics. With the relentless miniaturization of device technology, the ability to perform this characterization on the nanoscale is paramount. Due to its ability to probe electromechanical properties on the micro- and nano-scales, piezoresponse force microscopy (PFM) has become the premier tool for piezoelectric and ferroelectric characterization of a new generation of smart, functional materials. Despite its widespread use and popularity, PFM is a highly nuanced technique, and measurements on similar samples using different machines and/or in different laboratories often fail to agree. A comprehensive protocol for accurate quantitative measurements has not been presented in the literature, slowing the general uptake of the technique by reducing the ability of research groups to take full advantage of PFM for their characterization needs. Here, we present a procedure for PFM measurements, which outlines the practical aspects of quantitative PFM, from sample preparation to probe choice and use of control samples, and we substantiate these steps with original data on lithium niobate control samples. This quantitative characterization protocol is critical as society looks to smaller, greener alternatives to traditional piezoelectric materials for applications such as drug delivery, bio-microelectromechanical system sensors and actuators, and energy harvestin