Box plots display examples of genes significantly hyper or hypomethylated in ACC.

<p><i>EPHX3</i> and <i>MEIS</i> genes are significantly hypermethylated in ACC compared with <i>TMEM132D</i> and <i>ADCY2</i> which are significantly hypomethylated.</p

Unsupervised clustering analysis of normal and ACC samples using DML.

<p>Clustering analysis revealed separation of ACC and normal samples. Samples are on the horizontal axis: normal samples are shown with a yellow bar and ACC samples are shown with a blue bar.</p

Significantly regulated genes in the WNT pathway.

<p>Significantly regulated genes in the WNT pathway.</p

Methylation/expression correlations according to discretization method.

<p>For each gene, the upper heatmap represents the log2 methylation values for 14 ACC samples each normalized to the average of 6 normal adrenal samples. Log2 methylation ratios >0 represent hypermethylation and <0 represent hypomethylation. The lower heat map shows expression of z-transformed expression levels, where a value 0 indicates no expression change compared to average expression level of 14 ACC samples. Three genes with negative correlations were selected for visualization: <i>CCDC8</i> (TP53 pathway), <i>TBX3</i> (WNT pathway), and <i>PAX8</i> (other known cancer gene, involved in invasion and migration). Samples with higher methylation (peach-maroon) had lower expression (green). Samples with lower methylation (blue) had higher expression (red). Only the correlated methylation loci and expression probes are shown, and samples are organized by their discretization classification of M or U for each gene.</p

Frequency of DML by chromosome after normalization to chromosome length.

<p>Frequency of DML by chromosome after normalization to chromosome length.</p