36 research outputs found
Identification of Zoophilic Dermatophytes Using MALDI-TOF Mass Spectrometry
Dermatophytoses represent a major health burden in animals and man. Zoophilic
dermatophytes usually show a high specificity to their original animal host but a
zoonotic transmission is increasingly recorded. In humans, these infections elicit highly
inflammatory skin lesions requiring prolonged therapy even in the immunocompetent
patient. The correct identification of the causative agent is often crucial to initiate a
targeted and effective therapy. To that end, matrix assisted laser desorption ionization
time-of-flight mass spectrometry (MALDI-TOF MS) represents a promising tool. The
objective of this study was to evaluate the reliability of species identification of zoophilic
dermatophytes using MALDI-TOF MS. The investigation of isolates from veterinary clinical
samples suspicious of dermatophytoses suggests a good MALDI-TOF MS based
identification of the most common zoophilic dermatophyte Microsporum canis.
Trichophyton (T.) spp. usually achieved scores only around the cutoff value for secure
species identification because of a small number of reference spectra. Moreover, these
results need to be interpreted with caution due to the close taxonomic relationship of
dermatophytes being reflected in very similar spectra. In our study, the analysis of 50
clinical samples of hedgehogs revealed no correct identification using the provided
databases, nor for zoophilic neither for geophilic causative agents. After DNA
sequencing, adaptation of sample processing and an individual extension of the inhouse
database, acceptable identification scores were achieved (T. erinacei and
Arthroderma spp., respectively). A score-oriented distance dendrogram revealed
clustering of geophilic isolates of four different species of the genus Arthroderma and
underlined the close relationship of the important zoophilic agents T. erinacei, T.
verrucosum and T. benhamiae by forming a subclade within a larger cluster including
different dermatophytes. Taken together, MALDI-TOF MS proofed suitable for the
identification of zoophilic dermatophytes provided fresh cultures are used and the
reference library was previously extended with spectra of laboratory-relevant species.
Performing independent molecular methods, such as sequencing, is strongly
recommended to substantiate the findings from morphologic and MALDI-TOF MS
analyses, especially for uncommon causative agents
A Comparative Transcriptome Analysis of Human and Porcine Choroid Plexus Cells in Response to Streptococcus suis Serotype 2 Infection Points to a Role of Hypoxia
Streptococcus suis (S. suis) is an important opportunistic pathogen, which can cause
septicemia and meningitis in pigs and humans. Previous in vivo observations in S. suisinfected
pigs revealed lesions at the choroid plexus (CP). In vitro experiments with primary
porcine CP epithelial cells (PCPEC) and human CP epithelial papilloma (HIBCPP) cells
demonstrated that S. suis can invade and traverse the CP epithelium, and that the CP
contributes to the inflammatory response via cytokine expression. Here, next generation
sequencing (RNA-seq) was used to compare global transcriptome profiles of PCPEC and
HIBCPP cells challenged with S. suis serotype (ST) 2 infected in vitro, and of pigs infected
in vivo. Identified differentially expressed genes (DEGs) were, amongst others, involved in
inflammatory responses and hypoxia. The RNA-seq data were validated via quantitative
PCR of selected DEGs. Employing Gene Set Enrichment Analysis (GSEA), 18, 28, and 21
enriched hallmark gene sets (GSs) were identified for infected HIBCPP cells, PCPEC, and
in the CP of pigs suffering from S. suis ST2 meningitis, respectively, of which eight GSs
overlapped between the three different sample sets. The majority of these GSs are
involved in cellular signaling and pathways, immune response, and development,
including inflammatory response and hypoxia. In contrast, suppressed GSs observed
during in vitro and in vivo S. suis ST2 infections included those, which were involved in
cellular proliferation and metabolic processes. This study suggests that similar cellular
processes occur in infected human and porcine CP epithelial cells, especially in terms of
inflammatory response
Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy
Bacterial pathogens cause severe infections worldwide in livestock and in humans, and antibiotic resistance further increases the importance of prophylactic vaccines. Inactivated bacterial vaccines (bacterins) are usually produced via incubation of the pathogen with chemicals such as formaldehyde, which is time consuming and may cause loss of immunogenicity due to the modification of structural components. We evaluated low-energy electron irradiation (LEEI) as an alternative method to generate a bacterin. Rodentibacter pneumotropicus, an invasive Gram-negative murine pathogen, was inactivated with LEEI and formaldehyde. LEEI resulted in high antigen conservation, and LPS activity was significantly better maintained when compared with formaldehyde treatment. Immunization of mice with LEEI-inactivated R. pneumotropicus elicited a strong immune response with no detectable bacterial burden upon sublethal challenge. The results of this study suggest the inactivation of bacteria with LEEI as an alternative, fast and efficient method to generate bacterial vaccines with increased efficacy
The immunoglobulin M-degrading enzyme of Streptococcus suis, Ide Ssuis , is involved in complement evasion
A distinct variant of the SzM protein of Streptococcus equi subsp. zooepidemicus recruits C1q independent of IgG binding and inhibits activation of the classical complement pathway
ABSTRACTStreptococcus equi subsp. zooepidemicus (SEZ) is a major equine pathogen that causes pneumonia, abortion, and polyarthritis. It can also cause invasive infections in humans. SEZ expresses the M-like protein SzM, which recruits host proteins such as fibrinogen to the bacterial surface. Equine SEZ strain C2, which binds only comparably low amounts of human fibrinogen in comparison to human SEZ strain C33, was previously shown to proliferate in equine and human blood. As the expression of SzM_C2 was necessary for survival in blood, this study investigated the working hypothesis that SzM_C2 inhibits complement activation through a mechanism other than fibrinogen and non-immune immunoglobulin binding. Loss-of-function experiments showed that SEZ C2, but not C33, binds C1q via SzM in IgG-free human plasma. Furthermore, SzM C2 expression is necessary for recruiting purified human or equine C1q to the bacterial surface. Flow cytometry analysis demonstrated that SzM expression in SEZ C2 is crucial for the significant reduction of C3b labelling in human plasma. Addition of human plasma to immobilized rSzM_C2 and immobilized aggregated IgG led to binding of C1q, but only the latter activated the complement system, as shown by the detection of C4 deposition. Complement activation induced by aggregated IgG was significantly reduced if human plasma was pre-incubated with rSzM_C2. Furthermore, rSzM_C2, but not rSzM_C33, inhibited the activation of the classical complement pathway in human plasma, as determined in an erythrocyte lysis experiment. In conclusion, the immunoglobulin-independent binding of C1q to SzM_C2 is associated with complement inhibition
Immunogenicity of an Autogenous Streptococcus suis Bacterin in Preparturient Sows and Their Piglets in Relation to Protection after Weaningâ–ż â€
Streptococcus suis is an important porcine pathogen causing meningitis and other invasive diseases in piglets of different ages. Application of S. suis serotype 2 bacterins to specific-pathogen-free (SPF) weaning piglets has been demonstrated to protect against the homologous serotype. However, autogenous S. suis bacterins are also applied to sows and suckling piglets in the field. Therefore, comparative evaluation of different bacterin immunization regimes, including sow vaccination, was performed in this study. The main objectives were to determine the immunogenicity of an S. suis bacterin in sows prepartum and its influence on active immunization of piglets. Experimental infection of 6- and 8-week-old weaning piglets was performed to elucidate protective efficacies. Humoral immune responses were investigated by an enzyme-linked immunosorbent assay (ELISA) measuring muramidase-released protein (MRP)-specific IgG titers and by opsonophagocytosis assays. Bacterin application elicited high MRP-specific IgG titers in the serum and colostrum of sows, as well as opsonizing antibodies. Piglets from vaccinated sows had significantly higher MRP-specific titers than respective piglets from nonvaccinated sows until 6 weeks postpartum. Vaccination of suckling piglets did not result in high MRP-specific titers nor in induction of opsonizing antibodies. Furthermore, neither vaccination of suckling nor of weaning piglets from immunized sows was associated with a prominent active immune response and protection at 8 weeks postpartum. However, protection was observed in respective 6-week-old weaning piglets, most likely because of protective maternal immunity. In conclusion, this study provides the first results suggesting protective passive maternal immunity for S. suis serotype 2 after bacterin vaccination of sows and a strong inhibitory effect on active immunization of suckling and weaning piglets, leading to highly susceptible growers
Effect of Early-Life Treatment of Piglets with Long-Acting Ceftiofur on Colonization of Streptococcus suis Serotype 7 and Elicitation of Specific Humoral Immunity in a Farm Dealing with Streptococcal Diseases
In newborn piglets treatment with long-acting ceftiofur is a common approach to reduce losses due to streptococcal diseases on farms, even if problems start after weaning. The purpose of this study was to examine the influence of a single early-life treatment on Streptococcus (S.) suis colonization, transmission, immunoreaction, and drug resistance over an observation period of 14 weeks. In a farm with a history of streptococcal disease and isolation of a S. suis cps 7 mrp+, arcA+ isolate from diseased piglets, half of each litter was treated with a long-acting ceftiofur on day 1. S. suis-isolates were profiled and serum samples were tested for opsonizing antibodies. Treated and untreated pigs did not differ according to average daily weight gains, S. suis-isolation rates and level of opsonizing antibodies. Although the invasive cps 7 strain was not detected in a single piglet over 14 weeks, all animals developed bactericidal activity. No resistance to ceftiofur, but resistance to tetracyclins (100%), and trimethoprim/sulfamethoxazole (53%) was shown. Our results indicate that early treatment with ceftiofur does not prevent colonization and transmission of S. suis or the induction of bactericidal humoral immunity in nursery and fattening pigs. The necessity of continuous usage should be reconsidered
Streptococcus suis cps7: an emerging virulent sequence type (ST29) shows a distinct, IgM-determined pattern of bacterial survival in blood of piglets during the early adaptive immune response after weaning
Abstract Streptococcus (S.) suis is an important porcine pathogen causing meningitis, arthritis and septicemia. As cps7 emerged recently in Germany in association with severe herd problems, the objective of this study was to characterize the geno- and phenotype of invasive cps7 strains. Twenty cps7 strains were isolated from diseased pigs from different farms with S. suis herd problems due to meningitis and other pathologies. Eighteen of the cps7 isolates belonged to sequence type (ST) 29. Most of these cps7 strains secreted a short MRP variant in agreement with a premature stop codon. Expression of Ide Ssuis , an IgM specific protease, was variable in four further investigated cps7 ST29 isolates. Bactericidal assays revealed very high survival factors of these four cps7 ST29 strains in the blood of weaning piglets. In growing piglets, the increase of specific IgM led to efficient killing of cps7 ST29 as shown by addition of the IgM protease Ide Ssuis . Finally, virulence of a cps7 ST29 strain was confirmed in experimental infection of weaning piglets leading to meningitis and arthritis. In conclusion, this study characterizes cps7 ST29 as a distinct S. suis pathotype showing high survival factors in porcine blood after weaning, but IgM-mediated killing in the blood of older growing piglets. This underlines the relevance of IgM as an important host defense mechanism against S. suis