Morphometric measurements.

<p>A: Lumen diameter (µm) in vessels divided in groups of non-muscularized-, partially- and fully-muscularized vessels. B: Wall/lumen ratio divided in groups of non-muscularized-, partially- and fully-muscularized vessels. C: Number of non-muscularized-, partially- and muscularized vessels in each experimental group. D: Wall area of the vessels divided in groups of vessel diameter. * P<0.05 vs. wild type and ^# P<0.05 vs. normoxia. Statistical interaction was observed in Figure (B) for non-muscularized vessels.</p

Lung mRNA expression levels of A) eNOS, K_Ca2.1, K_Ca2.2, K_Ca2.3, K_Ca3.1, and K_Ca1.1; B) α-Smooth muscle actin (α-SMA), collagen-1, and TGFβ.

<p>Data are given as means ±SEM, n = 7–8. Data were analyzed by two-way ANOVA and differences were considered significant when *P<0.05 from wild type, ^# P<0.05 from normoxia. Statistical interaction (£) was observed in K_Ca2.3 expression (A). C–E) Genotyping: C:Gel electrophoresis shows that polymerase chain reacton (PCR) detected the K_Ca2.3-wild type allele (wild type (+)) and the tTA allele (T) in K_Ca2.3^T/+, and K_Ca2.3^T/T. D and E: PCR detected the targeted allele in K_Ca3.1^−/+ and in K_Ca3.1^−/− as well as the wild type allele in K_Ca3.1^+/− and K_Ca3.1^+/+. A DNA ladder was used to determine products sizes.</p

Characteristics of the animals.

<p>Selected morphological and functional characteristics and hematocrit of the normoxic and hypoxic strains. Values are means ±SEM, n = 7–8. BPM =  beats per minute. BW =  body weight. *P<0.05 vs. wild type;</p>#<p>P<0.05 vs. normoxia; 2-way ANOVA (n = 7–8 per group).</p

Right ventricular systolic blood pressure and hypertrophy.

<p>A) The effect of chronic hypoxia on right ventricular systolic blood pressure (RVSBP) in wild type and K_Ca3.1^−/−/K_Ca2.3^T/T(+Dox) mice. Values are means ±SEM, normoxic wild type (n = 8) and K_Ca3.1^−/−/K_Ca2.3^T/T(+Dox) mice (n = 7). B) Representative trace of right ventricular pressure measurements in normoxic wild type mice (top) and normoxic K_Ca3.1^−/−/K_Ca2.3^T/T(+Dox) mice (bottom). C) Hypoxia induced right ventricular hypertrophy as indicated by alterations of the weight ratio of right ventricle/left ventricle + septum, in wild type and K_Ca3.1^−/−/K_Ca2.3^T/T(+Dox) mice, n = 8. D) The effect of hypoxia on right ventricular wall thickness/heart weight (HW) in wild type and K_Ca3.1^−/−/K_Ca2.3^T/T(+Dox) mice. Values are mean ±SEM, n = 8. Data were analyzed by 2−way ANOVA and differences were considered significant when *P<0.05 vs. wild type, ^# P<0.05 vs. normoxia.</p