Identification of genomic regions associated with agronomic and biofortification traits in DH populations of rice.

Rice provides energy and nutrition to more than half of the world's population. Breeding rice varieties with the increased levels of bioavailable micronutrients is one of the most sustainable approaches to tackle micronutrient malnutrition. So, high zinc and iron content in the grain are primary targets in rice biofortification breeding. In this study, we conducted QTL mapping using doubled haploid (DH) populations, PSBRc82 x Joryeongbyeo and PSBRc82 x IR69428, phenotyped for agronomic traits and micronutrients during two growing seasons and using genotypic information from analysis with the 6K SNP chip. A number of DH lines were identified as having high grain Zn and Fe content in polished rice. Importantly, we identified 20 QTLs for agronomic traits and 59 QTLs for a number of biofortification traits. Of the 79 QTLs, 12 were large-effect QTLs (>25% PVE), nine QTLs were consistent across seasons in either population, and one QTL was identified in both populations. Moreover, at least two QTLs were clustered in defined regions of chromosomes 1, 2, 3, 4, 5, 7 and 9. Eight epistatic interactions were detected for Cu, Mg, Na, and Zn in population 1. Furthermore, we identified several candidate genes near QTLs for grain Zn (OsNRAMP, OsNAS, OsZIP, OsYSL, OsFER, and OsZIFL family) and grain yield (OsSPL14 and OsSPL16). These new QTLs and candidate genes help to further elucidate the genetic basis for grain micronutrient concentration, and may prove useful for marker assisted breeding for this important trait

Additional file 1: Table S1. of Advances in breeding for high grain Zinc in Rice

List of Zinc homeostasis genes and their putative functions Chen et al. 2008; Dräger et al. 2004; Gross et al. 2003; Grotz & Guerinot 2006; Kobayashi et al. 2003; Kobayashi et al. 2005; Koike et al. 2004; Lee et al. 2010a; Lee et al. 2010b; Ricachenevsky et al. 2011; Sasaki et al. 2012; Stein et al. 2009; Takahashi et al. 2012; Vasconcelos et al. 2008; Yang et al. 2007; Yang et al. 2009a; Yang et al. 2009b; Yokosho et al. 2009; Yuan et al. 2012. (DOC 44 kb