Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model-8

Fferent expression patterns in the transverse plane are symbolized by different fillings of the bars. Both cathepsins exhibited the same spatial and temporal distribution pattern up to 35 d after transection.<p><b>Copyright information:</b></p><p>Taken from "Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model"</p><p>http://www.biomedcentral.com/1471-2202/9/80</p><p>BMC Neuroscience 2008;9():80-80.</p><p>Published online 12 Aug 2008</p><p>PMCID:PMC2527007.</p><p></p

Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model-1

Le spinal cord sections (A, B), in the dorsal horn (DH) (C-F), the layer IX of the ventral horn (VH) and the fasciculus gracilis (FG). In the FG immunopositive cells exhibit macrophage-like morphology (K, M) and in the VH small immunopositive cells engulf motoneurons (G, I). At this time point the ipsilateral nucleus gracilis exhibits more intense CATS- (L) and CATX-staining (N) than the contralateral side. Scale bars, 500 μm (A, B, L, N), 20 μm (C-J), 10 μm (K, M).<p><b>Copyright information:</b></p><p>Taken from "Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model"</p><p>http://www.biomedcentral.com/1471-2202/9/80</p><p>BMC Neuroscience 2008;9():80-80.</p><p>Published online 12 Aug 2008</p><p>PMCID:PMC2527007.</p><p></p

Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model-6

L5 segment. CATS-immunopositive deposits are localized in small glial-like cells (C, E, F) that distributed homogenously throughout the section (A), while CATX is mostly found in large neurons (D, H) and only few small cells are intensely stained (D, J). G, I: Sections incubated with preabsorbed primary antibodies are free of immunostaining. Scale bars, 500 μm (A, B), 50 μm (C, D), 20 μm (E-J).<p><b>Copyright information:</b></p><p>Taken from "Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model"</p><p>http://www.biomedcentral.com/1471-2202/9/80</p><p>BMC Neuroscience 2008;9():80-80.</p><p>Published online 12 Aug 2008</p><p>PMCID:PMC2527007.</p><p></p

Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model-5

Er GFAP (A'-A"') and colocalization of CATX with the microglial marker PT66 (B'-B"') and the macrophage marker ED1 (C'-C"'). Large motoneurons expressed CATX (D) and CATS (E). Scale bars, 10 μm (A, B), 5 μm (C), 20 μm (D, E).<p><b>Copyright information:</b></p><p>Taken from "Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model"</p><p>http://www.biomedcentral.com/1471-2202/9/80</p><p>BMC Neuroscience 2008;9():80-80.</p><p>Published online 12 Aug 2008</p><p>PMCID:PMC2527007.</p><p></p

Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model-4

density of PT66-immunopositive microglial cells (C-E) and GFAP-immunopositive astrocytes (F-H) is also found in the ipsilateral dorsal (DH; D, G) and ventral horn (VH; E, H). Scale bars, 500 μm (A, C, F), 20 μm (B, D, E, G, H).<p><b>Copyright information:</b></p><p>Taken from "Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model"</p><p>http://www.biomedcentral.com/1471-2202/9/80</p><p>BMC Neuroscience 2008;9():80-80.</p><p>Published online 12 Aug 2008</p><p>PMCID:PMC2527007.</p><p></p

Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model-2

Fferent expression patterns in the transverse plane are symbolized by different fillings of the bars. Both cathepsins exhibited the same spatial and temporal distribution pattern up to 35 d after transection.<p><b>Copyright information:</b></p><p>Taken from "Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model"</p><p>http://www.biomedcentral.com/1471-2202/9/80</p><p>BMC Neuroscience 2008;9():80-80.</p><p>Published online 12 Aug 2008</p><p>PMCID:PMC2527007.</p><p></p

Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model-0

L5 segment. CATS-immunopositive deposits are localized in small glial-like cells (C, E, F) that distributed homogenously throughout the section (A), while CATX is mostly found in large neurons (D, H) and only few small cells are intensely stained (D, J). G, I: Sections incubated with preabsorbed primary antibodies are free of immunostaining. Scale bars, 500 μm (A, B), 50 μm (C, D), 20 μm (E-J).<p><b>Copyright information:</b></p><p>Taken from "Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model"</p><p>http://www.biomedcentral.com/1471-2202/9/80</p><p>BMC Neuroscience 2008;9():80-80.</p><p>Published online 12 Aug 2008</p><p>PMCID:PMC2527007.</p><p></p

Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model-3

Sham operated animals (n = 5) at 8 d after injury. Expression levels were normalized relative to the corresponding α-tubulin band. At this time point the L5 transection induced an upregulation of both proteins in all SC segments. The expression level of CATX was substantially higher than that of CATS. Data are means ± SD. CATX activities in the lumbar SC 8 d after transection in L5T, sham (n = 7, for each group) and naive animals (n = 4). Each symbol represents the value of a single animal, the bar indicates the mean for the group. CATX activity was significantly higher in L5T than in sham or naive SC. *** p ≤ 0.001. L, lumbar; C, cervical; T, thoracic.<p><b>Copyright information:</b></p><p>Taken from "Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model"</p><p>http://www.biomedcentral.com/1471-2202/9/80</p><p>BMC Neuroscience 2008;9():80-80.</p><p>Published online 12 Aug 2008</p><p>PMCID:PMC2527007.</p><p></p

Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model-7

Le spinal cord sections (A, B), in the dorsal horn (DH) (C-F), the layer IX of the ventral horn (VH) and the fasciculus gracilis (FG). In the FG immunopositive cells exhibit macrophage-like morphology (K, M) and in the VH small immunopositive cells engulf motoneurons (G, I). At this time point the ipsilateral nucleus gracilis exhibits more intense CATS- (L) and CATX-staining (N) than the contralateral side. Scale bars, 500 μm (A, B, L, N), 20 μm (C-J), 10 μm (K, M).<p><b>Copyright information:</b></p><p>Taken from "Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model"</p><p>http://www.biomedcentral.com/1471-2202/9/80</p><p>BMC Neuroscience 2008;9():80-80.</p><p>Published online 12 Aug 2008</p><p>PMCID:PMC2527007.</p><p></p