Response of macrophages to OMVs.

<p>Macrophages sense <i>L</i>. <i>pneumophila</i> OMVs via TLR2, which is critical for the initial activation of macrophages, and which results in IRAK-1 degradation. The downstream NF-κB nuclear translocation and signaling is essential for the induction of cytokine gene expression and miR-146a induction. A subsequent infection of OMV pre-treated macrophages with <i>Legionella</i> results in a higher rate of bacterial replication concurrent with more vacuoles per cell. While pro-inflammatory gene expression caused by OMVs cannot be further enhanced by <i>L</i>. <i>pneumophila</i> infection, there is induced transcription of anti-apoptotic genes. This results in increased viability of infected cells. Prolonged IRAK-1 absence, via protein degradation and suppressed translation by miR-146a, leads to a more efficient replication of <i>L</i>. <i>pneumophila</i>.</p

<i>Legionella pneumophila</i>-Derived Outer Membrane Vesicles Promote Bacterial Replication in Macrophages

<div><p>The formation and release of outer membrane vesicles (OMVs) is a phenomenon of Gram-negative bacteria. This includes <i>Legionella pneumophila</i> (<i>L</i>. <i>pneumophila</i>), a causative agent of severe pneumonia. Upon its transmission into the lung, <i>L</i>. <i>pneumophila</i> primarily infects and replicates within macrophages. Here, we analyzed the influence of <i>L</i>. <i>pneumophila</i> OMVs on macrophages. To this end, differentiated THP-1 cells were incubated with increasing doses of <i>Legionella</i> OMVs, leading to a TLR2-dependent classical activation of macrophages with the release of pro-inflammatory cytokines. Inhibition of TLR2 and NF-κB signaling reduced the induction of pro-inflammatory cytokines. Furthermore, treatment of THP-1 cells with OMVs prior to infection reduced replication of <i>L</i>. <i>pneumophila</i> in THP-1 cells. Blocking of TLR2 activation or heat denaturation of OMVs restored bacterial replication in the first 24 h of infection. With prolonged infection-time, OMV pre-treated macrophages became more permissive for bacterial replication than untreated cells and showed increased numbers of <i>Legionella</i>-containing vacuoles and reduced pro-inflammatory cytokine induction. Additionally, miRNA-146a was found to be transcriptionally induced by OMVs and to facilitate bacterial replication. Accordingly, IRAK-1, one of miRNA-146a’s targets, showed prolonged activation-dependent degradation, which rendered THP-1 cells more permissive for <i>Legionella</i> replication. In conclusion, <i>L</i>. <i>pneumophila</i> OMVs are initially potent pro-inflammatory stimulators of macrophages, acting via TLR2, IRAK-1, and NF-κB, while at later time points, OMVs facilitate <i>L</i>. <i>pneumophila</i> replication by miR-146a-dependent IRAK-1 suppression. OMVs might thereby promote spreading of <i>L</i>. <i>pneumophila</i> in the host.</p></div

OMVs reduce responsiveness of macrophages to <i>L</i>. <i>pneumophila</i> infection.

<p>(A-D) THP-1 cells treated as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005592#ppat.1005592.g002" target="_blank">Fig 2A</a> and RNA samples were taken at the time of infection (0 h) or 24 and 48 h post infection (p.i.). qPCR was performed for markers of classically activated macrophages (A: IL-1β, B: TNF-α, C: IL-6) and alternatively activated macrophages (D: CD206). Results are normalized to untreated control cells at every time point. Mean values of three independent experiments are shown. <u>Statistics:</u> Mann-Whitney test; * p<0.05 compared to 0 μg/mL OMVs. # p<0.05 compared to equally treated 0 h sample.</p

<i>L</i>. <i>pneumophila</i> OMVs induce cytokine secretion in macrophages.

<p>(A) THP-1 cells were incubated with rising amounts of OMVs (0.01, 0.05, 0.1, 1, 5, 10, 25 μg/mL) for 24 or 48 h, respectively, or left untreated for control. Supernatant was collected for IL-8 ELISA. (B-E) THP-1 cells were incubated with rising amounts of OMVs (0.1, 1, 10 μg/mL) for 24 or 48 h, respectively, or left untreated for control. Supernatant was collected for magnetic multiplex ELISA. Results for IL-6 (B), TNF-α (C), IL-1β (D), and IL-10 (E) are shown. (F) mBMDM (wildtype (WT) and TLR2/4^-/-) were stimulated with OMVs (0.1, 1 μg/mL) for 24 or 48 h, respectively, or left untreated for control. Supernatant was collected for CXCL1 ELISA. Mean values of three independent experiments are shown. <u>Statistics:</u> Mann-Whitney test; *p<0.05 and **p<0.01 compared to corresponding control. #p<0.05 compared to equally treated WT sample.</p

OMVs induce miR-146a upregulation which augments bacterial replication.

<p>(A+B) THP-1 cells were pre-incubated with OMVs (0.1, 1, 10 μg/mL) or LPS/IFN-γ for 20 h and then infected with <i>L</i>. <i>pneumophila</i> (MOI 0.5) for 24 h and 48 h, respectively. qPCR was performed for miR-146a (A) and pri-mir-146a (B). Mean values of three independent experiments are shown. (C+D) THP-1 cells were transfected with miR-146a mimic or inhibitor and corresponding controls. After 24 h of transfection, cells were PMA-differentiated and RNA samples were taken after 24 h incubation. qPCR for miR-146a was performed (C). (D) Transfected THP-1 cells were then additionally infected with <i>L</i>. <i>pneumophila</i> (MOI 0.5) and lysed for CFU 24 and 48 h p.i., respectively. Results are depicted relative to control transfected cells. Mean values of three independent experiments are shown. <u>Statistics:</u> A+B: Mann-Whitney test; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 compared to corresponding 0 μg/mL OMVs. # p<0.05 compared to equally treated 0 h sample. C+D: Mann-Whitney test; *p<0.05, ***p<0.001 compared to corresponding control transfected sample. # p<0.05 compared to miR-146a mimic transfection.</p

OMVs lead to IRAK-1 degradation and knockdown of IRAK-1 enhances <i>L</i>. <i>pneumophila</i> replication.

<p>A) THP-1 cells were pre-incubated with OMVs (0.1, 1, 10 μg/mL) or LPS/IFN-γ for 20 h and then infected with <i>L</i>. <i>pneumophila</i> (MOI 0.5) for 24 h and 48 h, respectively. The expression of IRAK-1 was determined by western blot. One representative out of three biological replicates is shown. (B-G) THP-1 cells were transfected with siRNA targeting IRAK-1 or a scramble control. After 24 h of transfection, THP-1 cells were PMA-differentiated. (B) RNA and protein samples were taken after the indicated time points. Expression of IRAK-1 was analyzed by qPCR and western blot. Mean values of four independent experiments are shown for qPCR, one representative out of three biological replicates is shown for western blot. (C) Transfected and PMA-differentiated THP-1 cells were additionally infected with <i>L</i>. <i>pneumophila</i> (MOI 0.5) for 24 and 48 h and then lysed for CFU assay. Results are depicted relative to scramble control. Mean values of four independent experiments are shown. (D-F) qPCR for markers of classically activated macrophages (IL-1β (D), TNF-α (E), and IL-6 (F)) and alternatively activated macrophages (CD206 (G)) are presented relative to the time point of infection (0 h p.i.) in scramble transfected cells. Mean values of four independent experiments are shown. <u>Statistics:</u> B-G: Mann-Whitney test; **p<0.01, ***p<0.001 compared to corresponding scramble transfected sample.</p

Blocking of NF-κB signaling prior to OMV treatment reduces bacterial replication and M1 activation.

<p>THP-1 cells were pre-incubated with IKK inhibitor (1 μM) 90 min before OMV stimulation (1 μg/mL). DMSO was used as a solvent control. After 20 h of OMV incubation, cells were infected with <i>L</i>. <i>pneumophila</i> (MOI 0.5) and lysed for CFU 24 and 48 h p.i.. Results are depicted relative to CFU count of not pre-treated (but infected) cells (A). RNA samples were taken at the time point of infection (0 h) or 24 and 48 h p.i.. qPCR was performed for IL-1β (B), TNF-α (C), IL-6 (D), and CD206 (E). Results are calculated relative to the time point of infection (0 h) with 1 μg/mL OMV treatment. Mean values of three independent experiments are shown. Symbol key is used for all parts of the figure. <u>Statistics:</u> A: Mann-Whitney test; *p<0.05 compared to 0 μg/mL OMVs. #p<0.05 compared to 1 μg/mL OMVs. B-E: Mann-Whitney test; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 compared to 1 μg/mL OMV treatment.</p

Screening for eukaryotic motifs in <i>Legionella pneumophila</i> reveals Smh1 as bacterial deacetylase of host histones

Legionella pneumophila (L.p.) is a bacterial pathogen which is a common causative agent of pneumonia. In humans, it infects alveolar macrophages and transfers hundreds of virulence factors that interfere with cellular signalling pathways and the transcriptomic landscape to sustain its own replication. By this interaction, it has acquired eukaryote-like protein motifs by gene transfer events that partake in the pathogenicity of Legionella. In a computational screening approach for eukaryotic motifs in the transcriptome of Legionella, we identified the L.p. strain Corby protein ABQ55614 as putative histone-deacetylase and named it “suppressing modifier of histones 1” (Smh1). During infection, Smh1 translocated from the Legionella vacuole into the host cytosol. When expressed in human macrophage THP-1 cells, Smh1 was localized predominantly in the nucleus, led to broad histone H3 and H4 deacetylation, blunted expression of a large number of genes (e.g. IL-1β and IL-8), and fostered intracellular bacterial replication. L.p. with a Smh1 knockdown grew normally in media but showed a slight growth defect inside the host cell. Furthermore, Smh1 showed a very potent histone deacetylation activity in vitro, e.g. at H3K14, that could be inhibited by targeted mutation of the putative catalytic centre inferred by analogy with eukaryotic HDAC8, and with the deacetylase inhibitor trichostatin A. In summary, Smh1 displays functional homology with class I/II type HDACs. We identified Smh1 as a new Legionella virulence factor with a eukaryote-like histone-deacetylase activity that moderates host gene expression and might pave the way for further histone modifications. Legionella pneumophila (L.p.) is a prominent bacterial pathogen which is a common causative agent of pneumonia. In order to survive inside the host cell, the human macrophage, it profoundly interacts with host cell processes to advance its own replication. In this study, we identify a bacterial factor, Smh1, with yet unknown function as a host histone deacetylase. The activity of this factor in the host cell leads to attenuated gene expression and increased intracellular bacterial replication.</p