Production of Clay-Based Water Filter Using Biomass of Bacillus Subtillis, Sawdust, Activated Charcoal, Periwinkle and Snail Shell as Additives

A Significant proportion of rural households lacked access to improved and safe drinking water due to chemical and microbial contamination. Point-of-use (POU) water filters made from cheap, locally available ceramic materials and additives can achieve quality water parameters. Ceramic water filters were prepared by combining clay minerals with additives. Sawdust was used as a burnout material to achieve porosity and enhance the filtration rate. Silver nitrate, charcoal, periwinkle shell, snail shell, and biomass of Bacillus Subtilis were added in different ratios. The filter was formulated with charcoal, sawdust, snail shell, and periwinkle shell to remove microbes and treat heavy metals through the adsorption process. The filters were molded and fired in a temperature range of (700oC - 900oC). Characterization of the clay mineral, physiochemical and Microbial tests were conducted on the ceramic and water. Antimicrobial test was carried out on the biomass of Bacillus subtilis. Mineralogical (XRD) and elemental analysis of the clay, snail, and periwinkle shells showed high percentage composition of serpentine (a clay crystal), plagioclase, a mixture of feldspar minerals albite (sodium aluminosilicate - NaAlSi3O8), anorthite calcium aluminosilicate- CaAl2Si2O8) and Calcium (70-97 %composition) respectively. The results showed a greater proportion of silica in the clay, suggesting the material is silicate. Filtration rate was estimated at 1.125 L/hr. The result showed the filter has 96.72%, 99.26%, and 66.67% colony removal efficiency for heterotrophic bacteria, coliform, and fungi respectively. The filter showed about 70% - 96% efficiency for the treatment of physiochemical parameters in wastewater

Adaptation of the Wound Healing Questionnaire universal-reporter outcome measure for use in global surgery trials (TALON-1 study): mixed-methods study and Rasch analysis

BackgroundThe Bluebelle Wound Healing Questionnaire (WHQ) is a universal-reporter outcome measure developed in the UK for remote detection of surgical-site infection after abdominal surgery. This study aimed to explore cross-cultural equivalence, acceptability, and content validity of the WHQ for use across low- and middle-income countries, and to make recommendations for its adaptation.MethodsThis was a mixed-methods study within a trial (SWAT) embedded in an international randomized trial, conducted according to best practice guidelines, and co-produced with community and patient partners (TALON-1). Structured interviews and focus groups were used to gather data regarding cross-cultural, cross-contextual equivalence of the individual items and scale, and conduct a translatability assessment. Translation was completed into five languages in accordance with Mapi recommendations. Next, data from a prospective cohort (SWAT) were interpreted using Rasch analysis to explore scaling and measurement properties of the WHQ. Finally, qualitative and quantitative data were triangulated using a modified, exploratory, instrumental design model.ResultsIn the qualitative phase, 10 structured interviews and six focus groups took place with a total of 47 investigators across six countries. Themes related to comprehension, response mapping, retrieval, and judgement were identified with rich cross-cultural insights. In the quantitative phase, an exploratory Rasch model was fitted to data from 537 patients (369 excluding extremes). Owing to the number of extreme (floor) values, the overall level of power was low. The single WHQ scale satisfied tests of unidimensionality, indicating validity of the ordinal total WHQ score. There was significant overall model misfit of five items (5, 9, 14, 15, 16) and local dependency in 11 item pairs. The person separation index was estimated as 0.48 suggesting weak discrimination between classes, whereas Cronbach's α was high at 0.86. Triangulation of qualitative data with the Rasch analysis supported recommendations for cross-cultural adaptation of the WHQ items 1 (redness), 3 (clear fluid), 7 (deep wound opening), 10 (pain), 11 (fever), 15 (antibiotics), 16 (debridement), 18 (drainage), and 19 (reoperation). Changes to three item response categories (1, not at all; 2, a little; 3, a lot) were adopted for symptom items 1 to 10, and two categories (0, no; 1, yes) for item 11 (fever).ConclusionThis study made recommendations for cross-cultural adaptation of the WHQ for use in global surgical research and practice, using co-produced mixed-methods data from three continents. Translations are now available for implementation into remote wound assessment pathways