Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis-2

<p><b>Copyright information:</b></p><p>Taken from "Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis"</p><p>http://www.biomedcentral.com/1471-213X/7/123</p><p>BMC Developmental Biology 2007;7():123-123.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2190771.</p><p></p>with an anti-bovine MYST4. From left to right, Br, brain; Th, thymus; Mu, muscle; Lu, Lung; He, heart; Li, liver; Ki, kidney; Sp, spleen; Te, testicle; Ov, ovary; Ut, uterus; Oo, germinal vesicle stage oocytes. TUBULIN and β-ACTIN antibodies were incubated simultaneously on the same membrane and were used as control

Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis-4

<p><b>Copyright information:</b></p><p>Taken from "Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis"</p><p>http://www.biomedcentral.com/1471-213X/7/123</p><p>BMC Developmental Biology 2007;7():123-123.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2190771.</p><p></p> relative mRNA levels shown represent the quantity of transcript corrected for the value obtained for each pool. The highest level was attributed the relative value of 100. Shown is the relative mRNA abundance (mean ± SEM). Different letters indicate a significant difference of relative mRNA abundance (P < 0.05)

Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis-0

<p><b>Copyright information:</b></p><p>Taken from "Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis"</p><p>http://www.biomedcentral.com/1471-213X/7/123</p><p>BMC Developmental Biology 2007;7():123-123.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2190771.</p><p></p>main zinc finger; C2HC, zinc finger; MYST HAT, conserved HAT domain characteristic of MYST family members; Acidic, glutamate/aspartate-rich region; SM-rich, serine/methionine-rich domain. MYST4 is composed of 2054 residues in which the N-terminal region and the SM-rich domain encode transcriptional repression and activation domains respectively. B) Schematic illustration of MYST4 showing the alternative MYST4 splicing variants MORF and MORFα. Conserved regions between either 2 or 3 sequences are highlighted in gray and black respectively. Regions used for primer designs are underlined in blue (forward) and in red (reverse). The number of residues in each sequence is indicated on the right. (Accession numbers: MYST4; [GenBank; ], MORF; [GenBank; ], MORFα; [GenBank; ]

Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis-6

<p><b>Copyright information:</b></p><p>Taken from "Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis"</p><p>http://www.biomedcentral.com/1471-213X/7/123</p><p>BMC Developmental Biology 2007;7():123-123.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2190771.</p><p></p>s and round spermatids (C, D, E), round spermatids and elongating spermatids (F, G, H), round spermatids and elongated spermatids (I, J, K). Images D, G, K are enlargements of boxed sections in C, F, I respectively. In magnified sections, arrowheads indicate: round spermatocyte (D), nucleus (left) and tail (up) of an elongating spermatid (G) and nuclei of elongated spermatids located in inner (up) wall and inside (left) of the lumen (J). Positive sections were incubated with anti-MYST4 (A, C, D, F, G, I, J) and negatives were prepared by peptide-blocking assay (B, E, H, K). Original magnification 1000× (A, B)

Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis-3

<p><b>Copyright information:</b></p><p>Taken from "Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis"</p><p>http://www.biomedcentral.com/1471-213X/7/123</p><p>BMC Developmental Biology 2007;7():123-123.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2190771.</p><p></p>oocyte nuclei (C, D); portion of large antral follicles, arrowheads indicating granulosa cells (right), theca (down) and basal lamina (left) (E, F) and blood vessels (G, H). Positive sections were incubated with anti-MYST4 (A, C, E, G,) and negatives were prepared by peptide-blocking assay (B, D, F, H). Original magnifications: 200× (C, D, E, F, G, H) and 400× (A, B)

Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis-7

<p><b>Copyright information:</b></p><p>Taken from "Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis"</p><p>http://www.biomedcentral.com/1471-213X/7/123</p><p>BMC Developmental Biology 2007;7():123-123.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2190771.</p><p></p>main zinc finger; C2HC, zinc finger; MYST HAT, conserved HAT domain characteristic of MYST family members; Acidic, glutamate/aspartate-rich region; SM-rich, serine/methionine-rich domain. MYST4 is composed of 2054 residues in which the N-terminal region and the SM-rich domain encode transcriptional repression and activation domains respectively. B) Schematic illustration of MYST4 showing the alternative MYST4 splicing variants MORF and MORFα. Conserved regions between either 2 or 3 sequences are highlighted in gray and black respectively. Regions used for primer designs are underlined in blue (forward) and in red (reverse). The number of residues in each sequence is indicated on the right. (Accession numbers: MYST4; [GenBank; ], MORF; [GenBank; ], MORFα; [GenBank; ]

Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis-5

<p><b>Copyright information:</b></p><p>Taken from "Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis"</p><p>http://www.biomedcentral.com/1471-213X/7/123</p><p>BMC Developmental Biology 2007;7():123-123.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2190771.</p><p></p>ly blastocyst and blastocyst) stained with an anti-MYST4 antibody (green signal) and with propidium iodide (red signal) to visualize the DNA. Original magnification 600×