Supplementary document for Adaptive point-scan imaging beyond the frame rate-resolution limit with scene-reactive scan trajectories - 6084258.pdf

Supplemental Documen

Visualization 4: Aphids

Adaptive scanning of aphids in culture plate

Visualization 2: Multiple Targets

Adaptive scanning of multiple moving targets in simulation

Visualization 1: Single Target

Adaptive scanning of a single homogeneous moving target in simulation

Visualization 3: Coffee Grounds

Adaptive scanning of coffee grounds suspended in water

Visualization 5: Forceps

Adaptive scanning of vitreoretinal forceps over porcine retina

Representative anterior segment OCT motion images acquired from a healthy volunteer without tracking.

<p>(A) Single frame B-scans composed of 1000 A-lines. (B) Registered and averaged (10x) B-scan acquired in repetitive B-scan mode at a frame rate of 100 Hz. (C) Volumetric image composed of 1000 A-lines and 200 B-scans, corresponding to a volume frame rate of 0.5 Hz. Red arrow denotes significant motion artifacts due to patient motion. Scale bars are 1 mm. Yellow arrow denotes an artifact from specular reflection.</p

Optical performance of the anterior segment OCT system with and without tracking.

<p>(A-B) System diagram without tracking (A) and after 2.5 mm of lateral tracking (B). (C-D) Spot diagrams of the extrema of the +/- 15 mm tracking FOV. The airy radius was 39.5 μm as denoted by the black circles and the optical performance was diffraction limited.</p

Characterization of pupil tracking system for a 3 mm step response.

<p>(A) Generated FSM driving waveform (input) and response waveform generated by tracking algorithm (output), and the fit to the output step function. (B) Impulse response after calculating the derivative of the step response. (C) Magnitude of the frequency response of the system estimated by calculating the Fourier transform of the impulse response. The -3 dB amplitude was measured at 58 Hz, as denoted by the dashed red line.</p

Anterior segment volumetric time series acquired with volumes composed of 500 A-lines/B-scans and 200 B-scans/volume with and without tracking.

<p>(A) Volume corrupted by prominent motion artifacts before activating tracking. (B) Volume during which tracking was activated; image artifact caused by repositioning of the scanners is denoted by the red arrow. (C) Volume acquired with tracking activated. Scale bars are 1 mm.</p