Quantification of gold-labeled pKu70 and 53BP1 in cortical neurons analyzed by TEM. Induction.

<p>Quantification of pKu70 and 53BP1 cluster in euchromatic and heterochromatic domains at 5<b> </b>min and 40<b> </b>min after irradiation with doses ranging from 1 to 10 Gy. The induction of pKu70 (<b>A</b>) and 53BP1 cluster (<b>B</b>) was clearly dependent on the radiation dose, with a linear correlation in the dose range of 1 to 10 Gy. <b>Repair.</b> Quantification of pKu70 (<b>C</b>) and 53BP1 cluster (<b>D</b>) per nucleus at 5<b> </b>min, 20<b> </b>min, 40<b> </b>min, 5 h, 24 h, 48 h, and 72 h after irradiation (6Gy). 53BP1 clusters disappeared with kinetics similar to those observed with heterochromatin-associated pKu70 clusters.</p

Quantification of the different pKu70 and 53BP1 clusters in euchromatic and heterochromatic compartments of cortical neurons analyzed by TEM.

<p>(<b>A</b>) Quantification of pKu70 clusters consisting of 1, 2, 4, or ≥6 beads, analyzed separately in euchromatic and heterochromatic compartments at the different time-points after irradiation (6 Gy). We observed solely pKu70 clusters of 1 and 2 beads in euchromatin, but increasingly complex pKu70 clusters with 4 or ≥6 beads in heterochromatin. (<b>B</b>) Quantification of 53BP1 clusters <10 and ≥10 beads, analyzed in the heterochromatic compartment at different time-points after irradiation (6Gy). We observed huge 53BP1 clusters at late repair-times.</p

Gold-labeled pKu70 and 53BP1 in cortical neurons of brain analyzed 40 min after irradiation with 6Gy.

<p>TEM micrographs of double-labeling of pKu70 (10-nm beads) and p53BP1 (6-nm beads) at different magnifications (boxed regions are shown at higher magnifications in the following images). Complex pKu70 clusters (consisting of 4 gold beads) co-localizing with 53BP1 were observed in heterochromatic regions, but only isolated pKu70 clusters without any p53BP1 binding were observed in euchromatic regions (pKu70 beads are marked by red arrows).</p

Correlation between the pre-RT IL-6 and TGF-β1 plasma levels, respectively, and the IL-6 and TGF-β1 staining intensity of the corresponding tumour biopsies (grade 1–4) (IL-6: no grade 4 samples).

<p>For both cytokines, statistically significant correlations were found between the amount of pre-RT plasma levels and the staining intensity of the corresponding tumour biopsies.</p

TNF-α, IL-1β, IL-6 and TGF-β1 plasma levels at the onset of radiation pneumonitis (mean values), depicted separately for patients with moderate (grade I/II) (n = 14) and severe lung toxicity (grade III/IV) (n = 7).

<p>Error bars represent SEs of the means. Gray-shaded area indicates the normal range of the cytokine plasma levels.</p

Correlations between the IL-6 and TGF-β1 ratios and the incidence of radiation pneumonitis.

<p>For every patient, the ratios of the plasma levels at the end of radiotherapy (end-RT) and before RT (pre-RT) are plotted logarithmically (base 10): relative values >imply an increase (supposed high risk for RP), values <1 imply a decrease of the plasma levels at the end of RT (supposed low risk for RP). The ratios were plotted separately for the patients without radiation pneumonitis (grade 0) (n = 31), and with moderate (grade I/II) (n = 14) and severe (grade III/IV) (n = 7) lung toxicity.</p

Results of the two-way anova with repeated measures and interactions (p-values of pairwise tests for the measures of quality).

<p>Results of the two-way anova with repeated measures and interactions (p-values of pairwise tests for the measures of quality).</p