Proteins specifically identified in one extraction fraction and common to the three gravity conditions (i.e. 1 g ground, 1 g space and µg space).

<p>Blue bars indicate the total number of proteins identified. Red bars show the number of proteins with at least one transmembrane segment and the green bars the number of proteins which are annotated as “membrane” in Gene Ontology (The number of proteins is indicated at the top of the bars).</p

Schematic view of the experiment scenario of Arabidopsis culture on board the ISS.

<p>Schematic view of the experiment scenario of Arabidopsis culture on board the ISS.</p

List of membrane proteins significantly under-represented in microgravity conditions on board the ISS.

<p>List of membrane proteins significantly under-represented in microgravity conditions on board the ISS.</p

Distribution according their putative functions of the 69 proteins under-represented in microgravity (percentage of the total).

<p>Distribution according their putative functions of the 69 proteins under-represented in microgravity (percentage of the total).</p

Workflow of microsome preparation and sequential extraction of proteins with salts and detergents.

<p>¹ Volumes of buffer are expressed as µl per mg of initial fresh material used. * Centrifugation 100 000 g, 20 min; ** Centrifugation 15 000 g 15 min.</p

Design and views of the culture chamber and of the experiment container used during the GENARA-A experiment.

<p>(A, C) View of a culture chamber, B. View of an experiment container housing four culture chambers. The grey part on the scheme corresponds to the filter pad with a nylon mesh sewed on top (A). Empty culture chamber (C-left) and closed culture chamber with the Biofoil (C-right). (Adapted from the Space Biology Product Catalog of ASTRIUM company).</p

Comparative distribution of under and over-represented proteins according to functional categories.

<p>Black bars = over-represented, Grey bars = under-represented.</p

Proteins specifically identified in one extraction fraction and common to the three gravity conditions (i.e. 1 g ground, 1 g space and µg space).

<p>Blue bars indicate the total number of proteins identified. Red bars show the number of proteins with at least one transmembrane segment and the green bars the number of proteins which are annotated as “membrane” in Gene Ontology (The number of proteins is indicated at the top of the bars).</p

Distribution according to their putative functions of the 80 proteins over-represented in microgravity (percentage of the total).

<p>Distribution according to their putative functions of the 80 proteins over-represented in microgravity (percentage of the total).</p

Venn diagrams of proteins identified on the basis of at least two peptides.

<p>A. Number of proteins, B. Number of corresponding sequenced peptides from microsomal samples isolated from seedlings grown on board the ISS in microgravity (i), 1 g on centrifuge (ii) or 1 g on Earth (iii). Total number of proteins identified is 3861 and total number of corresponding peptides is 13 708.</p