Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-3

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>bsence (Ctr) or presence of 0.5 μM BIO or 0.5 μM MeBIO. Ten days later, cells were stained with Oil Red O for adipocytes or with Alizarin red for osteoblasts (left panel). Effects of the compounds on GPDH, expressed in adipocytes only, and ALP, expressed in osteoblasts only, enzymatic activities are shown (right panel). Bars are the means ± SE of 3 independent experiments. *: p < 0.05, **: p < 0.0

Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-0

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>aled after treatment with 0.5 μM BIO or 0.5 μM MeBIO for 24 hours. DAPI was used to label nuclei (A). hMADS2 and hMADS3 cells were maintained as in (A) and fold induction in the expression of Gli1 gene was quantified by real-time PCR 24 hours after treatment with 0.5 μM BIO, 0.5 μM MeBio, 20 mM LiCl or 20 mM NaCl or under control condition (Ctr). Bars are the means ± S.E of 2 independent experiments, **: p < 0.01 (B)

Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-6

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>aled after treatment with 0.5 μM BIO or 0.5 μM MeBIO for 24 hours. DAPI was used to label nuclei (A). hMADS2 and hMADS3 cells were maintained as in (A) and fold induction in the expression of Gli1 gene was quantified by real-time PCR 24 hours after treatment with 0.5 μM BIO, 0.5 μM MeBio, 20 mM LiCl or 20 mM NaCl or under control condition (Ctr). Bars are the means ± S.E of 2 independent experiments, **: p < 0.01 (B)

HMADS3 cells were induced to undergo differentiation into the indicated lineages in the presence of BIO and MeBIO during the first 3 days

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p> Then, compounds were withdrawn and GPDH and ALP enzymatic activities were quantified at day 10. *: p < 0.05, **: p < 0.01, NS: not significant (A). hMADS3 cells were induced to undergo differentiation into adipocytes (upper panel) or osteoblasts (lower panel) as in A) and RNAs were prepared at day ten. The expression of molecular markers was checked by qPCR (B). Results are the log2 inductions of BIO treated cells versus MeBIO treated cells. Expression data are colour-coded according to the scale that is displayed

Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-2

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>er cell plating, 0.5 μM BIO was added to the culture medium. Fifteen days later, the number of colonies was scored. Bars represent means of 2 independent experiments. **: p < 0.01, ***: p < 0.00

Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-4

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>sence of 0.5 μM BIO or 0.5 μM MeBIO. Ten days later, RNAs were prepared and expression of adipogenic or osteogenic markers was checked by qPCR. Results are the log2 inductions of BIO treated cells versus MeBIO treated cells. Expression data are colour-coded according to the scale

Current agri-environmental policies dismiss varied perceptions and discourses on management of traditional rural biotopes

Traditional rural biotopes (TRBs) are threatened habitats that host significant biodiversity and several ecosystem services, and depend on active management such as low-intensity grazing. The current study explores private landowners’ decision-making on TRB management and abandonment within a social-ecological system framework. We provide insight into supporting resilience of TRB systems in the face of agricultural modernization. Using a mixed methods approach with content analysis and Q analysis, we demonstrate that TRB management fosters cultural, biological, aesthetic, and utilitarian values. These are reflected in different ways through conservationist’s, profit-oriented farmer’s, landscape manager’s, and landscape admirer’s discourses on TRB management. Overall, management reinforces landowners’ place attachment, and reflects an approach to landscapes as spatial representations of cultural heritage and identity over multiple generations. Landowners consider TRB pasturage and its social-ecological outcomes motivating and rewarding. Giving up grazing cattle and perceived bureaucracy of national agri-environment scheme contribute to TRB abandonment. Landowners point out that current policies detach TRB management from what is seen as “regular agriculture”, and the focus on monetary compensation bypasses the multiple values tied to TRB management. Based on our results, we suggest that promoting TRBs requires reconfiguring the current arrangement of remedial management payments and adopting a more participatory governance approach. Locally, resilience of TRB systems relies on the connections between landowners and landscapes that foster sense of place and landscape identity, which can be supported by knowledge sharing and collaborative grazing efforts among landowners.peerReviewe

Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation-1

Sp, dashed area), secreted proteins without signal peptide (ex, white area) and intracellular proteins (in, dark area). The percentage of the proteins present in each group is reported in the scheme. One hundred per cent is referred to a total number of 73 identified proteins. . Venn diagram of proteins expressed under the different culture conditions; n represents protein number in each condition.<p><b>Copyright information:</b></p><p>Taken from "Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation"</p><p>http://www.biomedcentral.com/1471-2199/9/26</p><p>BMC Molecular Biology 2008;9():26-26.</p><p>Published online 26 Feb 2008</p><p>PMCID:PMC2279142.</p><p></p

Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation-3

(), BIGH3 (), PTX3 () and PAI-1 () has been analyzed by Western blot. The bar graphs report the levels of expression of every single candidate as the mean of three independent experiments after 6 h of incubation. The values are indicated as arbitrary units. *: p < 0.05. Two μg of secreted proteins have been loaded for each gel.<p><b>Copyright information:</b></p><p>Taken from "Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation"</p><p>http://www.biomedcentral.com/1471-2199/9/26</p><p>BMC Molecular Biology 2008;9():26-26.</p><p>Published online 26 Feb 2008</p><p>PMCID:PMC2279142.</p><p></p

Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation-0

At day 3 and day 14 of differentiation as compared to day 0. The data are representative of three independent experiments. Microphotographs of hMADS cells differentiated into adipocytes and osteoblasts at day 14. Bar scale = 50 μm. Representative gel of secreted proteins from hMADS cells at day 0 and day 3 of differentiating adipocytes (adipo) and osteoblasts (osteo) after 6 h of incubation. The gel is representative of 3 independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation"</p><p>http://www.biomedcentral.com/1471-2199/9/26</p><p>BMC Molecular Biology 2008;9():26-26.</p><p>Published online 26 Feb 2008</p><p>PMCID:PMC2279142.</p><p></p