Characteristics of the study population (n = 1519, NephroTest cohort, 2000–2010, France).

<p>Abbreviations: SD, standard deviation; mGFR, measured glomerular filtration rate; CKD, chronic kidney disease; PCR, protein/creatinine ratio.</p><p>Characteristics of the study population (n = 1519, NephroTest cohort, 2000–2010, France).</p

Graphical representation of the illness-death model (Model M4).

<p>Graphical representation of the illness-death model (Model M4).</p

Association between sex, age, mGFR, and proteinuria at inclusion and hazard of progression to CKD stage 5 and death.

<p>Abbreviations: CKD, chronic kidney disease; HR, hazard ratio; CI, confidence interval; mGFR, measured glomerular filtration rate; PCR, protein/creatinine ratio.</p><p>*M1, Weibull model imputing the time to progression to CKD stage 5 at the time at the first mGFR measure below 15 mL/min/1.73 m², or censoring at death or latest news (n = 1519 patients contributed to the analysis); M2, Weibull model for death before CKD stage 5 diagnosis, censoring at the time at the first mGFR measure below 15 mL/min/1.73 m² (n = 1519); M3, Weibull model for death after CKD stage 5 diagnosis (n = 245); M4, Weibull illness-death model accounting for interval censoring (n = 1519).</p><p>Association between sex, age, mGFR, and proteinuria at inclusion and hazard of progression to CKD stage 5 and death.</p

Probabilities of progression to CKD stage 5 in the next t years after inclusion into the cohort for a man with different ages, levels of mGFR and proteinuria at inclusion, estimated from the illness-death model for interval-censored data (IMID, model M4, solid line) and the standard survival model (model M1, dotted line).

<p>Probabilities of progression to CKD stage 5 in the next t years after inclusion into the cohort for a man with different ages, levels of mGFR and proteinuria at inclusion, estimated from the illness-death model for interval-censored data (IMID, model M4, solid line) and the standard survival model (model M1, dotted line).</p

Effect of the down regulation of IQGAP1 expression on F- actin network.

<p>(A) IQGAP1 Western blot analysis showing IQGAP1 expression in podocytes transfected with siRNA IQGAP1 as compared with untransfected podocytes (UT) or podocytes transfected with siRNA directed at luciferase (Luc). (B) F-actin labelling after siRNA IQGAP1 transfection in comparison to control cells (untransfected cells). Scale bars, 50 µm.</p

Cellular localization of interactions between IQGAP1 and different proteins of the slit diaphragm: In situ Proximity Ligation assay.

<p>In situ Proximity Ligation assays confirmed in cells the interactions between IQGAP1 and nephrin, MAGI-1, CD2AP, podocin, NCK1/2 and podocalyxin. Cells were transfected with siRNA Luc (Luc) or siRNA IQGAP1 (siRNA).</p

Characteristics of the secondary antibodies used in the study.

<p>(Abbreviations: IH = immunohistochemistry, IF = Immunofluorescence, WB = Western blot, IP = Immunoprecipitation).</p

Involvement of IQGAP1 in podocyte proliferation, viability and adhesion.

<p>Influence of down regulation of IQGAP1 on cell proliferation and viability (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037695#pone-0037695-g007" target="_blank">Figure 7A and 7B</a>, n = 5, Two-way repeated measurements ANOVA). Cell survival was not altered by transfection. Increase of cytotoxicity was not the course of the migration defect. Podocyte adhesion after down regulation of IQGAP1 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037695#pone-0037695-g007" target="_blank">Figure 7C</a>, n = 3, Wilcoxon test). Adhesive properties of podocytes were conserved after siRNA IQGAP1 transfection. Control: control cells; siRNA: siRNA IQGAP1 transfected podocytes.</p

siRNA used in the study.

<p>siRNA used in the study.</p

Permeability assay.

<p>Permeability assays were performed by quantification of FITC-labelled dextran (70 kDa) passage through a podocyte layer seeded in a Transwell®. The fluorescence was measured at sequential times with a Wallac® plate reader. The down-regulation of IQGAP1 expression in podocytes by siRNA induced an increase of the dextran flux across the podocyte layer (data are representative of 4 independent experiments). Control: control cells; siRNA: siRNA IQGAP1 transfected podocytes.</p